FastQCFastQC Report
Sat 28 May 2022
EGAF00006134763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26220376
Sequences flagged as poor quality0
Sequence length96
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15064765.7454401111563005Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2488870.9492121699551523Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1074670.40986063662855177Clontech SMART CDS Primer II A (100% over 25bp)
GTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTT1035300.39484559641707656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG788260.30062879342386245No Hit
GGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGA564330.21522574657205526No Hit
CCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGG531440.20268206680178805No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT528930.20172479601360407Clontech SMART CDS Primer II A (100% over 23bp)
GCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCCCCC516310.19691174527779465No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480720.18333833199035743Clontech SMART CDS Primer II A (100% over 22bp)
GTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGAC428680.16349117190386592No Hit
GGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTG426300.16258348087761976No Hit
CTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTA403270.15380023535894374No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399750.15245776795878135Clontech SMART CDS Primer II A (100% over 21bp)
CCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCT398480.15197341182292734No Hit
ACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCA386890.14755318535477904No Hit
CCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTC385940.14719087170984885No Hit
GATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATA364230.13891105146623373No Hit
AAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTT359740.13719864276545846No Hit
CCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCC349070.13312928845871624No Hit
GGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTGTTAATGTC323290.12329724028366336No Hit
AAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG322200.122881533048954No Hit
CTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGA318910.12162678368914312No Hit
GGCTGCTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAAC313560.11958638579400997No Hit
GTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTA313250.11946815713092748No Hit
GACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTT302730.11545601024180584No Hit
CCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATA297610.1135033303870242No Hit
GTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCC284650.10856060950460818No Hit
CAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCT281810.10747748239765897No Hit
GAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGA280950.10714949320330112No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAT277740.10592525446622124No Hit
CGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTT267840.10214956490326454No Hit
CTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCT267510.10202370858449933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)