FastQCFastQC Report
Sat 28 May 2022
EGAF00006134831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34769927
Sequences flagged as poor quality0
Sequence length96
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19747185.679384946652318Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3352380.9641607818158491Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1503540.4324254117645976No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1424450.40967874335772986Clontech SMART CDS Primer II A (100% over 25bp)
GCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCA620000.17831501343100317No Hit
CTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTC498800.14345730435384577No Hit
GGAGTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCG481320.13842997139453297No Hit
GCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTC425490.12237299203993152No Hit
GTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCGCGA416180.11969539078986276No Hit
GGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGG401610.11550498797423417No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT377200.10848455333253934Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369480.106264243810463Clontech SMART CDS Primer II A (100% over 22bp)
CTCAGATCCACTGCCGCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGG348540.10024179803426105No Hit
GGAGCCATCATAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTCTGCTGAT348360.10019002915939397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)