FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006134867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26001777
Sequences flagged as poor quality0
Sequence length96
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14908205.733531212116772Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2457790.945239242687144Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1066000.4099719799919828Clontech SMART CDS Primer II A (100% over 25bp)
GTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTT1029420.3959037107348471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG785860.302233189677767No Hit
GGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGA562220.21622368348132517No Hit
CCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGG527140.2027322978733338No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT524600.20175544156078254Clontech SMART CDS Primer II A (100% over 23bp)
GCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCCCCC505610.19445209456261395No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477260.183548993593784Clontech SMART CDS Primer II A (100% over 22bp)
GGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTG419300.1612582093908428No Hit
GTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGAC417900.1607197846516413No Hit
CCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCT398990.15344720478142707No Hit
CTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTA395090.1519473072936515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393810.15145503324638157Clontech SMART CDS Primer II A (100% over 21bp)
CCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTC381800.14683611816223174No Hit
ACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCA379950.14612462832828693No Hit
GATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATA361740.13912126082767343No Hit
AAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTT356540.13712139751063937No Hit
CCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCC349960.13459080123639242No Hit
AAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG318240.12239163500248464No Hit
GGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTGTTAATGTC317740.12219934045276983No Hit
CTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGA317500.12210703906890671No Hit
GGCTGCTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAAC309570.11905724751042977No Hit
GTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTA306680.11794578501307813No Hit
GACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTT295640.11369992135537504No Hit
CCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATA294520.11326918156401386No Hit
GTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCC286440.1101617016406225No Hit
GAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGA279810.10761187591140406No Hit
CAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCT275370.10590430030993651No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAT273220.10507743374616281No Hit
CGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTT266570.1025199162349558No Hit
CTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCT262170.10082772419746543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)