FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347430
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA3049897.015386101673863RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1647963.790653328518228RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1299352.9887772776099903RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1025982.3599689931752783RNA PCR Primer, Index 2 (100% over 50bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG846261.9465753330128375RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT781491.7975907605182835RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA708821.6304345325859184RNA PCR Primer, Index 1 (100% over 22bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA634621.4597589840434464RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC634021.4583788583139923RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC617371.4200803693216453RNA PCR Primer, Index 1 (100% over 31bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA591311.360136908472362RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA509151.1711516919191338RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA463571.066308140671615RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG432510.9948636320768821RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA399880.919807794490078RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA382180.8790940854711864RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC382020.8787260519433321RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA362150.8330208882029153RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA346010.7958955060806039RNA PCR Primer, Index 1 (100% over 32bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG332400.7645896541174901RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC273430.6289462970076574RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC272590.6270141209864218RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC252850.5816079844873868RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC249670.5742933181212808RNA PCR Primer, Index 1 (100% over 33bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC241670.5558916417285614RNA PCR Primer, Index 2 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG198270.45606254729805884RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG185810.42740193631639845Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC182870.42063932024207407RNA PCR Primer, Index 1 (100% over 31bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT181000.4163379283852759RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA178260.4100353542207695RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA176110.4050899036902262RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC174880.40226064594484556RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA171150.3936808643267402RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG164780.37902852949903737RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA163210.3754172005069662RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC162000.3726339469525674RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA160580.36936764939285965RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC148730.3421101662361441RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA146170.3362216297904739RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG139440.32074121952509876RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC135620.31195441904757526RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT127050.2922416232118746RNA PCR Primer, Index 1 (100% over 30bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA124930.28736517896780395RNA PCR Primer, Index 1 (100% over 32bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG123320.28366184159376917Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC122210.28110860899427936RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA118940.2735869237687553RNA PCR Primer, Index 2 (100% over 36bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG114180.2626379263150873RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG114100.2624539095511601RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA109610.2521259686757463RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC109320.25145890790651027RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG104890.24126897960404192RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA100090.2302279737684103RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC99910.22981393604957412RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC98470.22650163429888465RNA PCR Primer, Index 2 (100% over 34bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA96550.222085231964632RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC95060.218657919736488RNA PCR Primer, Index 1 (100% over 31bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA94850.21817487573117914RNA PCR Primer, Index 2 (100% over 36bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT94150.21656472904681617RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC93470.21500058655343504RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT92630.2130684105321995RNA PCR Primer, Index 2 (100% over 39bp)
AACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC92010.2116422806117637RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC90970.2092500626807102RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC90940.2091810563942375RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG89910.2068118405586749Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT89680.2062827923623842RNA PCR Primer, Index 1 (100% over 30bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT88990.20469564777351218RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC88230.2029474885162038RNA PCR Primer, Index 1 (100% over 26bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT88050.20253345079736762RNA PCR Primer, Index 1 (100% over 30bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA87630.2015673627867499RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG83070.19107840724289984RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA82240.18916923331715518RNA PCR Primer, Index 1 (100% over 32bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC82130.1889162102667553RNA PCR Primer, Index 2 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT80680.18558090642057493RNA PCR Primer, Index 2 (100% over 37bp)
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCC80030.18408577021366646RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG79890.18376374087679387RNA PCR Primer, Index 2 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG78670.1809574852269042RNA PCR Primer, Index 1 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC78620.18084247474944967RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG77610.17851926310486885RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC77080.1773001520438512RNA PCR Primer, Index 1 (100% over 33bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC76580.17615004726930622RNA PCR Primer, Index 1 (100% over 27bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA76190.17525296554516118RNA PCR Primer, Index 2 (100% over 36bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG73470.16899639557163657No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG73380.16878937671221847RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC67210.15459708379433368RNA PCR Primer, Index 2 (100% over 34bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC67110.1543670628394247RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA66500.1529639350144798RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG65590.150870744324808RNA PCR Primer, Index 2 (100% over 38bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA65390.15041070241499002RNA PCR Primer, Index 1 (100% over 32bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG64070.1473744258101913RNA PCR Primer, Index 2 (100% over 35bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG63770.14668436294546433RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC63160.1452812351205195RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC62720.14426914291891993RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG60900.14008276153957624No Hit
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA60750.13973773010721277RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT60580.1393466944838675RNA PCR Primer, Index 1 (100% over 30bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC60310.1387256379056132RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC58990.1356893613008145RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC58470.13449325233528775RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA56280.12945579342278082RNA PCR Primer, Index 1 (100% over 23bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA53890.1239582926004559RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC53770.12368226745456512RNA PCR Primer, Index 1 (100% over 33bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA53520.12310721506729264RNA PCR Primer, Index 1 (100% over 32bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA53220.12241715220256565RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC51690.11889783159245808RNA PCR Primer, Index 1 (100% over 33bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT50850.11696565557122254RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC50370.11586155498765936RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA49650.11420540411231463RNA PCR Primer, Index 1 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA49590.11406739153936923RNA PCR Primer, Index 1 (100% over 28bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC47960.11031804997435266RNA PCR Primer, Index 2 (100% over 34bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA47880.11013403321042546RNA PCR Primer, Index 1 (100% over 28bp)
TCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAAC47650.10960498501413479RNA PCR Primer, Index 1 (100% over 24bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA47280.10875390748097151RNA PCR Primer, Index 1 (100% over 23bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG47080.10829386557115353Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG45880.10553361411224563RNA PCR Primer, Index 2 (100% over 35bp)
TTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAG45300.10419949257377348RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC44680.10277336265333771RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC44460.10226731655253794RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC44440.10222131236155615RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA43900.10097919920504757RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT43700.1005191572952296RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC43530.10012812167188431RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph