FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19897457
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA10139325.095786863617798RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC6948723.492265368383508RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA6694383.364439988486971RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA5919422.9749630819657003RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5097412.5618399376362517RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC4943072.484272236396842RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGC4093932.0575141838477147RNA PCR Primer, Index 28 (100% over 50bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC3677491.8482211068479755RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC3605351.811965217464724RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2863701.4392291437041427RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2802301.4083709290086668RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2662731.3382262869069148RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2425421.218959789685687RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2363251.1877145908645512RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2349161.180633283941762RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC2154121.0826107074889018RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1775510.8923301103251535RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1471640.7396121021897422RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1385760.6964508077590016RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1347310.6771267303153363RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA1142330.5741085406039576RNA PCR Primer, Index 2 (97% over 36bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC1074680.5401092209924112RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC974100.48956004779907303RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA947320.4761010414546944RNA PCR Primer, Index 7 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC902060.45335441609447885RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT889880.4472330308340408No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA883340.44394617864986463RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC811490.40783603653471895RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA802700.4034183865807575RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA763260.38359675811838667RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC748440.37614857014140046RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC729410.3665845338929492RNA PCR Primer, Index 1 (100% over 26bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA681530.3425211573519169RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG672980.33822412582673256RNA PCR Primer, Index 1 (100% over 29bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC632480.3178697659705962RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC612750.30795392597154503RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC609980.3065617882727426RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC578130.2905547176204477RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC576790.2898812647264422RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG526380.2645463689153845RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA521420.26205358805399104RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC505940.2542736993978678RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC492230.24738337165397567RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAA478040.24025180705252938RNA PCR Primer, Index 7 (97% over 37bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC474110.23827668028130428RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA471360.2368945941182333RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAG466640.23452243168561693RNA PCR Primer, Index 28 (100% over 39bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC464640.23351727811247439RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC438060.22015878712541007RNA PCR Primer, Index 1 (100% over 27bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA423530.2128563464165295RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG414950.208544237587748Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA403130.2026037799704756RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC393980.1980052023733485RNA PCR Primer, Index 2 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC388280.19514051468989227RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC381200.1915822710409677RNA PCR Primer, Index 1 (100% over 31bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT377740.18984335535943112RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC374010.18796874394552027RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA361000.18143021995222808RNA PCR Primer, Index 1 (100% over 28bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT360290.18107339043376247RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC357710.17977674232440857RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC354980.178404707697069RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA346700.17424337190425893RNA PCR Primer, Index 1 (100% over 32bp)
TCGTTTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA339220.17048409754070584RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA330180.16594080339010156RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA328930.16531258240688745RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC321240.1614477669181544RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG320920.1612869423464516RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC313430.15752264221503282RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG307030.15430615078097668RNA PCR Primer, Index 1 (100% over 29bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG306170.15387393474452538RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA304970.1532708426006399RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG302740.15215009636658594RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC301310.15143141156178905RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC300120.1508333451857692RNA PCR Primer, Index 1 (100% over 33bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT289940.14571711349847372RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG287750.14461647033588262RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA274610.13801261136033616RNA PCR Primer, Index 1 (100% over 32bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA274000.13770603952052768RNA PCR Primer, Index 2 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC271660.13653000983995092RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG271270.13633400489318812RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC262390.13187112302843523RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC256770.1290466414879047RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT256470.12889586845193335RNA PCR Primer, Index 1 (100% over 30bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC251400.126347804144017RNA PCR Primer, Index 2 (100% over 34bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG248690.12498582105240885RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC247470.12437267737279191RNA PCR Primer, Index 1 (100% over 26bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC243970.12261365861979247RNA PCR Primer, Index 1 (100% over 24bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC237870.11954794022170773RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC234040.11762307112913976RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG232500.11684910287782001RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAA231360.11627616534112878RNA PCR Primer, Index 28 (100% over 38bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA225380.11327075615743258RNA PCR Primer, Index 1 (100% over 22bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCC221510.11132578399340176RNA PCR Primer, Index 1 (100% over 27bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC221040.11108957290371327RNA PCR Primer, Index 1 (100% over 33bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA216510.10881290006054542RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT211700.10639550571713763RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT211200.10614421732385197RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA206510.10378713219483272RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC206080.10357102417660709RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA204910.1029830093363187No Hit
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT200930.10098275372576505RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC803650.069.8409966
TTCAACG804300.069.819365
CAACGCT804400.069.81927
TAGCAGC1145850.069.8050841
CAACGGA858650.069.793681
TGAGGTA1560200.069.777421
ATTCAAC808800.069.768334
TAGCTTA1121100.069.762821
ACGCTGT805500.069.758789
CAGCACG1129000.069.7540744
AACATTC892400.069.73171
ACGTAAA1134650.069.727288
CACGTAA1132600.069.720647
GCACGTA1132350.069.70536
CGCTGTC806350.069.69826510
GCAGCAC1154950.069.689313
CGTAAAT1135200.069.67219
GGTAGTA1645400.069.6443254
TATTGCA1137150.069.6433261
ATAGTTT1115950.069.6390817