FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14100424
Sequences flagged as poor quality0
Sequence length2-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGA277130.19654018914608526No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG271130.19228499795467144No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG216650.15364786193663396No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG212510.15071178001455843No Hit
CCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGA157790.11190443634886439No Hit
CAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAG141940.1006636396182129No Hit
AAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATC141810.1005714438090656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)