FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14426645
Sequences flagged as poor quality0
Sequence length2-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG280900.19470916488206372No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGA269890.18707745286586036No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG228160.15815180868455556No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG213500.14799005590003775No Hit
CCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGA153880.1066637461447204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC119350.036.1697047
TGTCGAG53300.033.5666929
CAGTCCG126000.033.492876
ACTCACG44150.033.43204145
GACAGTC119600.030.9787654
ACAGTCC128750.028.7773675
GTCCGCC153700.028.3519678
CTAGATA41400.027.7365655
GTAGCTG281400.027.673426145
GGTACTA37900.027.1650352
GGACAGT145850.026.9155963
TCCGCCC165550.026.7439929
GATAGAT76400.026.1485548
AGCCTAC56150.026.0932432
GCACCGT56950.025.447241
ATTATGC138500.025.048238
CAAGTAC358100.024.9155337
GCTAACC58450.024.7749315
TGCTAAC59150.024.768464
TAGATAG53000.024.3356576
Produced by FastQC (version 0.11.5)