FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5201677
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1525412.9325350266846635RNA PCR Primer, Index 6 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA965921.8569395985179395RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC826191.5883146915888855RNA PCR Primer, Index 6 (97% over 36bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA791601.5218169063553926RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC724011.3918780424082464RNA PCR Primer, Index 11 (100% over 50bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA676551.3006382364764286RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA628681.2086102232030171RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA546601.0508149583297848RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC462080.8883288985456036RNA PCR Primer, Index 11 (100% over 39bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT460620.8855221114267571RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA452150.8692389012235862RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA443180.8519944625550567RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG416170.8000689008563969RNA PCR Primer, Index 2 (97% over 35bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG405320.77921024315812Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC395180.759716529880652RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC383860.7379543174249381RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA371050.7133276441424563RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA342390.6582300285081139RNA PCR Primer, Index 1 (100% over 32bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC333020.6402166070672977RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA319030.6133214346065702RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT312520.600806239987604RNA PCR Primer, Index 11 (100% over 37bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC296420.569854683403064RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC253360.4870736879664001RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA234210.450258637743174No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA231660.4453563725698462RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG219280.4215563557675726RNA PCR Primer, Index 1 (100% over 29bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC204210.3925849298216709No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA201170.38674066075229196RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC190570.3663626172866943RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG190510.36624726987085127RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA185790.3571732731578681RNA PCR Primer, Index 11 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC175300.33700669995464927RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA169700.32624094114263535RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG167560.32212688331090145Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC166920.32089651087524274RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC162620.31262994607316064RNA PCR Primer, Index 1 (100% over 33bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG161350.3101884257711503Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC155310.29857678590962106RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG150520.28936821721148775No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA145910.28050569076088344RNA PCR Primer, Index 1 (100% over 32bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG129630.2492080919288145Illumina Small RNA Adapter 2 (100% over 21bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG126410.24301778061190651RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC117120.22515815572554773No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC111750.2148345620075987RNA PCR Primer, Index 1 (100% over 27bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA109820.21112422013131535RNA PCR Primer, Index 1 (100% over 22bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG106840.2053952984777794Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG104990.20183875315595337RNA PCR Primer, Index 1 (100% over 29bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA104520.2009351983985165RNA PCR Primer, Index 1 (100% over 22bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT103550.19907041517572122RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG102710.19745555135391915RNA PCR Primer, Index 1 (100% over 29bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA101350.19484100992814432RNA PCR Primer, Index 11 (100% over 40bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT101290.1947256625123013No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG97900.18820853351717148Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA96580.18567089036862536RNA PCR Primer, Index 1 (100% over 28bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA93150.17907686309626686RNA PCR Primer, Index 1 (100% over 22bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC92120.17709673245762855No Hit
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC88460.1700605400912052RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG86730.1667346896010652RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA85910.16515827491787746RNA PCR Primer, Index 1 (100% over 23bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT82870.15931400584849847No Hit
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA82030.15769914202669638RNA PCR Primer, Index 1 (100% over 32bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT77450.1488942892840136RNA PCR Primer, Index 11 (100% over 41bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG76840.14772159055627637RNA PCR Primer, Index 2 (97% over 35bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA76480.14702950606121834RNA PCR Primer, Index 1 (100% over 23bp)
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC72840.1400317628334093No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC72030.13847457271952873RNA PCR Primer, Index 6 (97% over 36bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG71650.13774403908585636No Hit
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA69690.1339760235016515RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG69480.13357230754620097RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA69110.13286099848183577RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA68900.13245728252638525No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA66830.12847779667980153RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC65150.12524806903619737RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC63900.12284499787280141RNA PCR Primer, Index 1 (100% over 27bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGG63620.12230670993220072Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC63110.12132625689753515RNA PCR Primer, Index 1 (100% over 31bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC60810.11690460595688659No Hit
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG59690.11475145419448382No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA58610.11267520070930971RNA PCR Primer, Index 1 (100% over 28bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGAA58570.11259830243208105RNA PCR Primer, Index 1 (100% over 23bp)
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA58470.11240605673900936RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC58250.11198311621425168RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC57810.1111372351647363RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC57730.11098343861027896RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA57440.11042592610037108RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC57160.10988763815977039RNA PCR Primer, Index 1 (100% over 31bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA57130.1098299644518489RNA PCR Primer, Index 1 (100% over 28bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG56820.1092340028033267No Hit
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC56280.10819587606073963RNA PCR Primer, Index 1 (100% over 33bp)
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGGGTGCCAA56220.10808052864489663No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG55690.10706162647161675Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG55550.1067924825013164No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC55240.10619652085279421RNA PCR Primer, Index 1 (100% over 27bp)
GTTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGA54940.10561978377357918RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG54390.10456243246168495RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA54180.10415871650623443RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA54030.10387034796662692No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGG53770.10337050916464055Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT53050.10198634017452449RNA PCR Primer, Index 1 (100% over 30bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG53030.10194789103591016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA207.931321E-569.9998552
ATAGTTT57200.069.8095817
TATTGCA253800.069.6543051
TCAACGC98400.069.642836
GTACCAG176500.069.61639414
TACCAGT175200.069.5936215
CAACGCT98600.069.5692147
CCCAACC176700.069.523144
TCCCAAC176300.069.5022053
TTGTACC177600.069.4814612
ATTCAAC98800.069.467144
CCAGTGT115100.069.4154317
TCAAGTA87300.069.398482
TGTACCA177700.069.3635813
CATTCAA98900.069.361523
TATAGTT80500.069.3410216
CTTGTAC177900.069.30527511
TAATCCA77600.069.2735447
GTAGTAG130700.069.221945
CTCCCAA177000.069.208892