FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006134937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17178836
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4815102.802925646417487RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT4671642.71941591386052RNA PCR Primer, Index 8 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA4250292.4741431840900048RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3661852.1316054242557527RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2662711.5499944233707104RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2580101.5019061827006206RNA PCR Primer, Index 1 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2346731.3660587946703722RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2281841.3282855718513176RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2099651.22223065637276RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC1988371.157453275646848RNA PCR Primer, Index 13 (100% over 50bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1931961.1246163593388983RNA PCR Primer, Index 1 (100% over 22bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG1898871.105354285936486RNA PCR Primer, Index 2 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1881771.095400177287914RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1849931.0768657434065962RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1812941.0553334346983696RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1776251.0339757594752055RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1315580.7658144009291432RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1218770.7094601752994207RNA PCR Primer, Index 1 (100% over 29bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1209560.7040989273079968RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1207510.702905598493402RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA964180.5612603787590731RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC903680.5260426259381019RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA902690.5254663354373952RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG750420.43682819953575436Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA719180.418643032624562RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA692560.4031472213833347RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA651150.37904197932851796RNA PCR Primer, Index 13 (100% over 39bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA613540.3571487614178283RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA589480.34314315591580247RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA547540.31872939470404166RNA PCR Primer, Index 1 (100% over 28bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA535000.3114297150284222RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG518090.3015862075870565RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC516850.3008643891821308RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC474690.27632256341465744RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG454920.26481421674902766RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC450570.26228203121561905RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG448010.2607918254764176RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC447750.26064047645602995RNA PCR Primer, Index 1 (100% over 27bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA445770.25948789545461637RNA PCR Primer, Index 13 (100% over 39bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC442270.2574505047955519RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG437070.254423524387799RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA434160.25272957958269116RNA PCR Primer, Index 1 (100% over 32bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG434010.25264226284015984Illumina Small RNA Adapter 2 (100% over 21bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC422040.2456743867861594RNA PCR Primer, Index 13 (100% over 37bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA413140.240493593395967RNA PCR Primer, Index 1 (100% over 28bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG410070.23870651073215904Illumina Small RNA Adapter 2 (100% over 21bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT404590.235516539071681RNA PCR Primer, Index 8 (97% over 36bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA403170.23468994057571768RNA PCR Primer, Index 1 (100% over 32bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA393510.22906674235669985RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG392550.2285079152044993No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC381100.22184273719127418RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC378050.22006729675980377RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA378040.22006147564363499RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG374190.21782034591866412Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC374160.21780288257015784RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA365010.21247656127574652RNA PCR Primer, Index 1 (100% over 28bp)
CTGCTGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC338450.19701567673153175RNA PCR Primer, Index 13 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC335930.19554875545700537RNA PCR Primer, Index 1 (100% over 27bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA335540.19532173192642388RNA PCR Primer, Index 1 (100% over 23bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA317600.18487864951967642RNA PCR Primer, Index 1 (100% over 32bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG317170.18462834152441993Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC305550.17786420453632598RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT298120.1735391152229406RNA PCR Primer, Index 1 (100% over 30bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG292730.17040153360798135Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG287730.1674909755236036RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA281280.16373635559475624RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG280260.16314260174554318RNA PCR Primer, Index 1 (100% over 29bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA280250.16313678062937442RNA PCR Primer, Index 1 (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC272990.1589106502908579RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT272520.15863705783092638RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG267260.15557515072616096RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA265160.15435271633072228RNA PCR Primer, Index 1 (100% over 34bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA259370.15098229006901281RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT258390.15041182068447478RNA PCR Primer, Index 1 (100% over 30bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA250530.1458364233758329RNA PCR Primer, Index 1 (100% over 23bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC249360.1451553527840885RNA PCR Primer, Index 1 (100% over 33bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA248530.1446722001420818RNA PCR Primer, Index 1 (100% over 28bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG246400.14343230239813687Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC246250.14334498565560552RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC245720.1430364664986615RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC245620.14297825533697395RNA PCR Primer, Index 1 (100% over 31bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA243020.14146476513309747RNA PCR Primer, Index 13 (100% over 38bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC242690.14127266829952856RNA PCR Primer, Index 1 (100% over 27bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG238300.13871719830144486No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC234080.13626068727823004RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG232800.13551558440862932RNA PCR Primer, Index 2 (97% over 35bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC231160.1345609213569534RNA PCR Primer, Index 1 (100% over 31bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC226310.13173768001510697RNA PCR Primer, Index 1 (100% over 33bp)
TTGCAGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC216860.12623672523563295RNA PCR Primer, Index 13 (100% over 37bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG213160.1240829122531934RNA PCR Primer, Index 2 (97% over 35bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT212580.12374528751540557RNA PCR Primer, Index 8 (97% over 36bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA212190.12351826398482413RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA211360.12303511134281742RNA PCR Primer, Index 1 (100% over 23bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA208870.12158565341679727RNA PCR Primer, Index 1 (100% over 32bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC207260.12064845371362763RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC204600.11910003681273865RNA PCR Primer, Index 1 (100% over 26bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA199330.11603230859180448RNA PCR Primer, Index 1 (100% over 32bp)
AGGTCGCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA198260.11540944916174764RNA PCR Primer, Index 13 (100% over 39bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG196860.11459449289812185RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTCGGGTGCCAAGGAA194870.1134360907805395RNA PCR Primer, Index 1 (100% over 23bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT181490.10564743734674457RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA178850.1041106626781931RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT175630.10223626327185381RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC173970.10126995798784039RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT173260.10085665873985875RNA PCR Primer, Index 13 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph