FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712156
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1867553.269431016940014RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1443302.526716707316817RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1102701.93044447665645RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC993051.7384854335210733RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA789861.3827703585126176RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA769421.3469870220631228RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA715541.25266186707786RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA649141.1364185431910474RNA PCR Primer, Index 1 (100% over 22bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC576331.0089535369832336RNA PCR Primer, Index 2 (100% over 34bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA568660.9955260325523322RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG553300.9686360106411659RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC496780.8696891331399212RNA PCR Primer, Index 1 (100% over 26bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA481960.8437444635615694RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG449590.7870758431667483RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA447270.7830143294405826RNA PCR Primer, Index 12 (100% over 39bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC443320.7760992521912917RNA PCR Primer, Index 2 (100% over 34bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA414060.7248751609724945RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC405840.7104847976840969RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC389190.681336434088985RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT351040.6145490424281129RNA PCR Primer, Index 12 (100% over 36bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA348770.610575061325356RNA PCR Primer, Index 1 (100% over 22bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG334370.5853656657836376Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA328610.5752819075669502RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA310060.5428073042823061RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA283800.49683517046803344RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG270580.4736915448387614RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC240090.420314151084109RNA PCR Primer, Index 12 (100% over 50bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC232730.4074293489183418RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG232480.4069916858012982No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC232010.40616887914125593RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC225960.3955774317087979RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC207750.36369805026333313RNA PCR Primer, Index 1 (100% over 26bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG207280.36287524360329093Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA202760.35496229444714045RNA PCR Primer, Index 1 (100% over 32bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT197140.34512362757599757RNA PCR Primer, Index 12 (100% over 38bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG193310.33841862862288774RNA PCR Primer, Index 12 (100% over 37bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC192690.33733322409261934RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC190190.3329565929221821RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC183290.32087709089177535No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT165090.2890152159709924RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC162280.28409588253542095RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG161600.28290543885706204RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA148750.2604095546410147No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA145490.2547024275947646RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA142820.2500281855047376RNA PCR Primer, Index 1 (100% over 28bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG132680.23227656947744424Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG129600.2268845598754656RNA PCR Primer, Index 1 (100% over 29bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG128450.22487130953706447Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG122400.2142798621046064Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA122200.2139297316109714No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA120580.2110936746125281RNA PCR Primer, Index 1 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC120550.21104115503848284RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC118770.20792499364513153RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG116530.2040035321164198RNA PCR Primer, Index 1 (100% over 29bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC115950.20298815368487835No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG113570.1988216008106221RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC110610.19363966950482445RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA110280.19306195419032673RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC109790.19220413448092105RNA PCR Primer, Index 1 (100% over 33bp)
CCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC109160.19110122342597086RNA PCR Primer, Index 2 (100% over 34bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC106440.18633944871253516RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC106330.18614687694103593RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT105600.18486890063926825RNA PCR Primer, Index 12 (100% over 43bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA104360.1826980915787314RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA102870.1800896194011508RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT99510.17420742710808318RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA96780.16942814586996574RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT96160.16834274133969732RNA PCR Primer, Index 1 (97% over 35bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA95590.1673448694328376RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC92260.16151519671381523RNA PCR Primer, Index 1 (100% over 27bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC90900.15913430935709738RNA PCR Primer, Index 1 (100% over 33bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT90890.15911680283241564No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA90190.15789134610469321RNA PCR Primer, Index 1 (100% over 28bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA85130.14903304461572828RNA PCR Primer, Index 1 (100% over 32bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA84990.1487879532701838RNA PCR Primer, Index 12 (100% over 40bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA83560.14628452024069372RNA PCR Primer, Index 1 (100% over 22bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT83300.14582935059896823RNA PCR Primer, Index 12 (100% over 36bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA82500.14442882862442832No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA79070.13842409065858846RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA77170.1350978509690562RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT76980.13476522700010293RNA PCR Primer, Index 1 (97% over 35bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT75710.13254189836552083No Hit
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC75490.13215675482252234RNA PCR Primer, Index 2 (100% over 34bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA75440.1320692221991136RNA PCR Primer, Index 1 (100% over 23bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC73580.1288130086083083RNA PCR Primer, Index 1 (100% over 33bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG73450.12858542378744559No Hit
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC73250.1282352932938106RNA PCR Primer, Index 2 (100% over 34bp)
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA71080.1244363774378711RNA PCR Primer, Index 1 (100% over 22bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT70850.12403372737019087RNA PCR Primer, Index 12 (100% over 41bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA70780.12391118169741862RNA PCR Primer, Index 1 (100% over 22bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT68730.1203223441376601RNA PCR Primer, Index 1 (97% over 35bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG68120.11925444613207342Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG67540.11823906770053198RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT67280.11778389805880653RNA PCR Primer, Index 1 (97% over 35bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG66420.11627833693617612RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA65500.11466773666545522RNA PCR Primer, Index 1 (100% over 32bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG65190.114125034400321No Hit
TCCCCGGCATCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA64620.1131271624934613RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA64250.11247942108023659RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC63780.11165661442019441RNA PCR Primer, Index 12 (100% over 42bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC63360.11092134038356094RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC62950.11020357287160926RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT62100.1087155182736606RNA PCR Primer, Index 12 (100% over 36bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA61590.1078226855148914RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC61160.1070699049535762RNA PCR Primer, Index 1 (100% over 27bp)
TCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC59790.1046715110721766RNA PCR Primer, Index 1 (100% over 33bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA59730.10456647192408611RNA PCR Primer, Index 1 (100% over 28bp)
CACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC58630.10264075420909373RNA PCR Primer, Index 2 (100% over 34bp)
CCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC58250.10197550627118729RNA PCR Primer, Index 2 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA57490.10064501039537435RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1300.070.016921
TGCATCA900.069.9973145
GGCACGA150.002226005869.99486519
CAATTGC252.8436789E-669.99486516
GCTCACG150.002226005869.99486517
CGTATTT455.456968E-1269.9942513
TCAACGC76100.069.859346
TATTGCA294150.069.636071
CAACGCT76350.069.627557
TGAGGTA241450.069.596441
TAGTAGA120300.069.590016
TACCAGT329050.069.5800615
GTACCAG331300.069.5611414
TATAGTT169000.069.5599816
CCAGTGT216600.069.558617
CCCAACC332050.069.554634
TTCAACG76450.069.539525
CGCTGTC76550.069.5364610
TCCCAAC331550.069.522293
TGTACCA333300.069.52173613