FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25804080
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA12888424.994721764930197RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA12794124.958177156480681RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC12428604.816525138660243RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA6970592.701351879237702RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA5847952.26628889694963RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC5251812.0352634157079037RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA5134391.9897589838506158RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4203481.6289982049350333RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC4191671.624421409327517RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA4118821.5961894398095187RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3939911.526855443015213RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC3924911.5210424087973684RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC3322211.2874746939243717RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC3315991.2850642224020388RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG3075211.1917533971371967RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2994911.160634287291002RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2889951.1199585491906705RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2345150.908829146398554RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC2226540.862863547159984RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1872960.7258387045769507RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1704640.6606087099404435RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1702280.6596941258901693RNA PCR Primer, Index 1 (100% over 32bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1572800.6095160145217345RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1534650.5947315308276832RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA1528780.5924566967704331RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC1520030.5890657601433572RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1423510.5516608226295997RNA PCR Primer, Index 1 (100% over 33bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC1370650.5311756900459152RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC1357010.5258897042638219RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1277140.49493723473187184RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1229720.47656029589119236RNA PCR Primer, Index 6 (100% over 34bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1123190.4352761268760599RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1107860.42933520590542273RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1034280.4008203353888223RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA1002590.38853933176458916RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC908980.3522621228890935RNA PCR Primer, Index 1 (100% over 33bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC821450.31834113054989754RNA PCR Primer, Index 1 (100% over 27bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA802560.31102058279155853RNA PCR Primer, Index 1 (100% over 32bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC774660.3002083391463676RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT752550.2916399267092646RNA PCR Primer, Index 1 (100% over 30bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA751920.2913957792721151RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA709660.27501852420237416RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC684000.2650743603337147RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTCCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA679180.2632064386717139RNA PCR Primer, Index 1 (100% over 32bp)
TCGTTTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA666060.2581219714091725RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA665210.25779256613682794RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA650220.2519834072751286RNA PCR Primer, Index 1 (100% over 32bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG641540.24861959814106915RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC618750.2397876614860906RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC613800.23786936019420188RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA585650.2269602326453801RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA584830.22664245344147124RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA559610.21686880524320185RNA PCR Primer, Index 1 (100% over 22bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC559000.21663240851834284RNA PCR Primer, Index 1 (100% over 33bp)
TCGTTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA557470.21603947902812268RNA PCR Primer, Index 1 (100% over 28bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC551610.21376852032701804RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC543590.21066048469854382RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT513440.19897628592067612RNA PCR Primer, Index 1 (100% over 30bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC496500.19241143261065694RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG487860.1890631249011784RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG482480.18697818329504481RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC473520.18350586418891895RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA462170.17910733496408318RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT458730.17777421245012417RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC455390.17647984349761744RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC454290.17605355432164216RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG449200.1740809980437202RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC443120.17172478150742052RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC439630.17037228221273537RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC438510.1699382423244696RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT432500.16760915328118653RNA PCR Primer, Index 1 (100% over 30bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC423590.16415621095578684RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC413820.1603699880018974RNA PCR Primer, Index 17 (100% over 50bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG412580.15988944383988887RNA PCR Primer, Index 6 (100% over 34bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC412090.15969955138877265RNA PCR Primer, Index 1 (100% over 31bp)
TCCCCGGCATCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA389480.15093737114440817RNA PCR Primer, Index 1 (100% over 32bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA387810.1502901866681548RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG379970.14725190745029468RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC365800.14176052779250412RNA PCR Primer, Index 1 (100% over 26bp)
CTCCTGGCTGGCTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA354200.13726511466403762RNA PCR Primer, Index 1 (100% over 32bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG353720.1370790975690666RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC351420.13618776565566373RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG340400.13191712318362056RNA PCR Primer, Index 1 (100% over 29bp)
AGTGTTTCCTACTTTATGGATGTGGAATTCTCGGGTGCCAAGGAACTCCA337980.13097928699647496RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT332980.1290416089238601RNA PCR Primer, Index 1 (97% over 35bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA326340.12646837244342757RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA325040.12596457614454767RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC313280.1214071573177575RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACATAATGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG313160.12136065304401475RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT312370.1210544999085416RNA PCR Primer, Index 1 (100% over 25bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA309060.11977175702447054RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC308370.1195043574504497RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA302890.1173806622828638RNA PCR Primer, Index 1 (100% over 32bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA302750.11732640729683057RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA301880.11698925131219558RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA299430.1160397890566143RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCATCACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACTC295840.11464853620047682RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC294810.11424937451751815RNA PCR Primer, Index 1 (100% over 33bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG293510.11374557821863829RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA292840.1134859293569079RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCC287440.11139323703848385RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC280820.10882775127034174RNA PCR Primer, Index 1 (100% over 26bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCC280200.1085874791893375RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG273710.10607237305108339Illumina Small RNA Adapter 2 (100% over 21bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT269460.10442534668936075RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC263740.10220864297428933RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC261400.10130180963630558RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC258240.10007719709441297RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph