FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124854
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA4646137.585699185645894RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3360225.486204242582763RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1741902.8439861586904764RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1527102.4932839215432727RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1441262.353133642042733RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1315842.148361414002685RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1173281.9156048454379484RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1005921.6423575157873151RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC893591.4589572257559118RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA858451.401584429604363RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC827261.3506607667709303RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG755351.233253886541622RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA752701.2289272527965565RNA PCR Primer, Index 1 (100% over 32bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG706971.1542642485845376RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA587460.9591412301419756RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA496890.8112683175794885RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC486740.7946964939898975RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA466390.7614712122117523RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA459910.7508913681860825RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC390440.6374682563861931RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG381820.6233944515248853RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA348730.5693686739308398RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT330110.5389679492768317RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG324810.5303146817867006RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC304260.49676286161270133RNA PCR Primer, Index 1 (100% over 26bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG283070.4621661185719692Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC275460.44974133260972426RNA PCR Primer, Index 1 (100% over 26bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC268940.4390961809048836RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC258920.4227366072725979RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG245780.40128303466498955No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG238220.3889398833017081RNA PCR Primer, Index 1 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG233800.38172338475333456Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA224140.365951580233586RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC212160.34639193032193094RNA PCR Primer, Index 2 (100% over 34bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC210780.3441388153905383RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG207020.3379998935484829RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA202450.3305384912032189RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC193300.31559935959289803RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA174050.28417003899194987RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA172680.2819332509803499RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG171450.27992503984584777RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA166230.27140238771405817RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC164080.26789209995862756RNA PCR Primer, Index 1 (100% over 26bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC162830.26585123498454005RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA157340.2568877560183475RNA PCR Primer, Index 1 (100% over 28bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA149520.24412010474045587RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA147970.2415894321725873RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA147310.24051185546626908RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC145110.23691993311187498RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC144080.23523826037322687RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC142740.233050453121005RNA PCR Primer, Index 30 (100% over 36bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA140280.22903403085200075RNA PCR Primer, Index 7 (100% over 35bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC136360.22263387829326214RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA132790.21680516792726814RNA PCR Primer, Index 1 (100% over 32bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC132300.21600514885742583RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG128860.2103886884487369Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC125160.20434772812543775RNA PCR Primer, Index 1 (100% over 33bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC119610.19528628764048905RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA117570.1919555960027782RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA115540.18864123128486002RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA114050.18620852023574766No Hit
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA108390.17696748363307926RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG105490.17223267689319616RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC104650.17086121563060933RNA PCR Primer, Index 1 (100% over 31bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC103690.16929383133051007RNA PCR Primer, Index 2 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT100140.16349777480410144RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA98390.1606405638403789RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG95460.1558567763411177RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC93930.15335875761283452RNA PCR Primer, Index 30 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC93030.1518893348314915RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC91550.14947295070217184RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA90690.14806883559999962RNA PCR Primer, Index 1 (100% over 32bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA90130.1471545280916084RNA PCR Primer, Index 7 (100% over 35bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG88370.14428099020809312Illumina Small RNA Adapter 2 (100% over 21bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA87820.1433830096194946RNA PCR Primer, Index 1 (100% over 23bp)
CAAAGAATTCTCCTTTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG85030.1388277989973312RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG84780.1384196260025137RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA80210.13095822365724963RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCC77080.1258478977621344RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC75530.12331722519426586RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT75290.12292537911924105RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC74460.12157024477644691RNA PCR Primer, Index 1 (100% over 33bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT74170.12109676410245862RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT73820.12052532190971409RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA73390.11982326435862797RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG73140.11941509136381048RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT72820.11889262993044405RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC70740.11549663061356238RNA PCR Primer, Index 1 (100% over 26bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA69550.11355372715823103RNA PCR Primer, Index 1 (100% over 22bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA69280.11311290032382812RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG68570.1119536890185464RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT61620.10060647976261965RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph