FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35092302
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA24568887.001216392130673RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA17458314.9749685842781135RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC6324671.8022955575841109RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG6257031.7830206750186979RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4874621.3890852757393914RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA4517701.287376359635797RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA4488701.2791124389616846RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4219221.2023206685044485RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA4159781.1853824807503366RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC3741541.0661996468627222RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3063360.8729435874568731RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC2982910.8500183316557575RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2902700.8271614669222898RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2865520.8165665506925136RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2643060.7531737302386148RNA PCR Primer, Index 2 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC2639640.7521991575246332RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2422980.6904591212055567RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA2190580.6242337706999103RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG2079790.5926627440969817RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2023000.5764797077148145RNA PCR Primer, Index 1 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG1857250.5292471266205334Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1851890.527719726109732RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1823830.5197236704505735RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1564290.4457644300450851RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGC1544710.4401848587761498RNA PCR Primer, Index 34 (100% over 39bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1522880.4339641212480162RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1438410.4098933150637995RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1392810.39689901221071217RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1368860.39007415358502273RNA PCR Primer, Index 1 (100% over 27bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG1314420.3745607797402405Illumina Small RNA Adapter 2 (100% over 21bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1299260.3702407439671527RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1290210.36766183079126585RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1289180.3673683191259439RNA PCR Primer, Index 2 (100% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1219040.34738102960586625No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1196310.34090382557405324RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT1162820.33136042200936267RNA PCR Primer, Index 9 (97% over 36bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1146300.3266528368529371RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1120100.3191868119680493RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1089140.3103643642414795RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1064210.3032602420895614RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGC1052220.29984353833498867RNA PCR Primer, Index 34 (100% over 50bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1049200.2989829507337535RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1011070.2881173198612049RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC997730.28431591635111314RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA908450.25887443918612124RNA PCR Primer, Index 1 (100% over 28bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA904600.2577773324759373RNA PCR Primer, Index 1 (100% over 32bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC900410.256583338419919RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATG897150.255654359751036RNA PCR Primer, Index 12 (97% over 37bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC847320.2414546643306558RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA842870.2401865799513523RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC821100.2339829401901306RNA PCR Primer, Index 1 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG813060.23169183942392835No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC801180.22830648157536088RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT794830.2264969679105121RNA PCR Primer, Index 9 (97% over 36bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG789690.22503225921172115RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGG767790.21879157428885687RNA PCR Primer, Index 34 (100% over 38bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA756280.2155116526695798RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG748380.2132604466928388RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA739480.21072427793423187RNA PCR Primer, Index 7 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG699240.1992573755919461RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA673230.19184549363561273RNA PCR Primer, Index 7 (100% over 35bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC672390.19160612489884532RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA669780.1908623720381752RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA659030.18779902213311625RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA657560.1873801268437733No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA643310.18331940720218354RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC643010.18323391836762376RNA PCR Primer, Index 1 (100% over 27bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC642360.1830486925594109RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT641330.18275518089408896RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG638370.18191169105976576RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC636800.18146429949223622RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC629150.1792843342109617RNA PCR Primer, Index 1 (100% over 33bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA606310.17277578427314344RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC585060.16672032515849203RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT582330.165942376763998No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA575630.16403312612549614No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC563270.160510986141633RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC563100.1604625424687158RNA PCR Primer, Index 1 (100% over 26bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG562360.160251670010135Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG561910.1601234367582953Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC560760.15979572955914947RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC556370.15854474294675797RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC533400.15199914784729712RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC510420.1454507031200176RNA PCR Primer, Index 2 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA505880.1441569720903462RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC490250.13970300380978143RNA PCR Primer, Index 1 (100% over 27bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA478780.136434480701779RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA464760.1324393025000184RNA PCR Primer, Index 1 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA464650.13240795659401314RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC462350.13175254219572144RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC462350.13175254219572144RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC448920.12792549203526177RNA PCR Primer, Index 1 (100% over 33bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT440910.1256429401525155RNA PCR Primer, Index 9 (97% over 36bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA439150.12514140565643145RNA PCR Primer, Index 1 (100% over 32bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA432330.12319795948410567RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT430930.12279901158949334RNA PCR Primer, Index 9 (97% over 36bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT426500.12153662646582716RNA PCR Primer, Index 1 (100% over 30bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA425070.12112912968775887RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA418480.11925122495526226No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT414640.11815696787289703RNA PCR Primer, Index 1 (100% over 30bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC412420.11752435049715462No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCAT405150.11545267107298918RNA PCR Primer, Index 34 (100% over 41bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC401950.11454079017101813RNA PCR Primer, Index 1 (100% over 26bp)
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA395960.1128338631076411RNA PCR Primer, Index 1 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG386350.11009537077390932RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA378580.10788120995881093RNA PCR Primer, Index 7 (100% over 35bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT377570.1075933975491263No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG373550.10644784716602519RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA373510.10643644865475056RNA PCR Primer, Index 7 (100% over 35bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG364970.10400286649761535RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT363670.10363241488118961RNA PCR Primer, Index 1 (100% over 30bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA363210.10350133200153129RNA PCR Primer, Index 7 (100% over 35bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATC353720.10079703520162342RNA PCR Primer, Index 34 (100% over 42bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA353520.10074004264525023RNA PCR Primer, Index 1 (100% over 22bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG352560.10046647837465893RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph