FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24689998
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC14425585.842681720751861RNA PCR Primer, Index 4 (100% over 50bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA8937333.6198180331970864RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA8753273.5452696269963244RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT6486582.6272096093324917RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG4587971.8582302031778215RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA3467461.4043986556823536RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3453191.3986189873324413RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3393111.3742852470056903RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3218681.303637205640924RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC2425120.9822277020840585RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2252360.9122560479753785RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2223180.9004374969977722RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2014610.8159619940025916RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1970690.798173414189827RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1879190.761113872913234RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1737780.7038396681927638RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1490970.6038761121001306RNA PCR Primer, Index 3 (100% over 34bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1479750.5993317617927713RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1291870.5232361703715003RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1272440.5153665869069735RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1241040.5026488864033121RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1169500.47367359041503365RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA1091320.44200894629477083RNA PCR Primer, Index 2 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1072510.434390476661845RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1065280.43146216536753057RNA PCR Primer, Index 4 (100% over 37bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1061770.4300405370628219RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1051890.426038916649568RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC988290.40027949779501804RNA PCR Primer, Index 4 (100% over 38bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA966720.3915431665891589RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC890970.36086272667984826RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC888610.3599068740305285RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA887100.35929529034388746RNA PCR Primer, Index 1 (100% over 32bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG855700.346577589840226Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA842510.3412353455840701RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG820880.3324747130396689RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG817720.33119484254312215RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC810430.3282422299102657RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC741590.3003604941563786RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA716820.2903280915616113RNA PCR Primer, Index 4 (100% over 39bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT709480.2873552278133032RNA PCR Primer, Index 1 (100% over 30bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG704140.28519240868306267RNA PCR Primer, Index 2 (97% over 35bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC688500.27885785976977395RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA673190.27265696821846647RNA PCR Primer, Index 1 (100% over 32bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG660390.26747268266283375Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA625870.25349131255498686RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG601840.24375862646890453RNA PCR Primer, Index 2 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC596790.2417132638082838RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG582850.2360672528203526RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC579800.23483193477779948RNA PCR Primer, Index 1 (100% over 26bp)
AGGTCGCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA569180.2305305978558605RNA PCR Primer, Index 4 (100% over 39bp)
CTGCTGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC564730.22872824858066004RNA PCR Primer, Index 4 (100% over 37bp)
TTGCAGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC564150.2284933356414205RNA PCR Primer, Index 4 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG561490.22741597629939053RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT527440.21362496667678954RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA523350.21196842543284128RNA PCR Primer, Index 1 (100% over 32bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA518970.2101944277192732RNA PCR Primer, Index 2 (97% over 36bp)
GTACATGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC516010.20899556168453315RNA PCR Primer, Index 4 (100% over 38bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC512610.20761848583381823RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA499690.20238559760110147RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC493590.19991496151599525RNA PCR Primer, Index 1 (100% over 26bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG493420.1998461077234595Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA451990.18306603346018901RNA PCR Primer, Index 1 (100% over 28bp)
AGGTCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA448680.18172540961728711RNA PCR Primer, Index 4 (100% over 39bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC440530.17842447779866163RNA PCR Primer, Index 1 (100% over 27bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT431670.17483598014062213RNA PCR Primer, Index 3 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG431250.17466587077082793No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC430540.17437830493141393RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT430080.17419199466925836RNA PCR Primer, Index 1 (100% over 30bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC429130.17380722347567626RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA423760.17163225367616475RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG421950.17089916329681357RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA417630.1691494669217875RNA PCR Primer, Index 2 (97% over 36bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT417540.16911301491397446RNA PCR Primer, Index 3 (100% over 34bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA408580.1654840150250316RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA400340.16214663119859307RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC394760.15988660671418442RNA PCR Primer, Index 1 (100% over 27bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC393600.1594167808357052RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG390530.15817336234697144RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA389660.15782099293811203RNA PCR Primer, Index 1 (100% over 28bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA382430.15489268164379763RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC375250.1519846214649349RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC373720.1513649373321132RNA PCR Primer, Index 1 (100% over 31bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT373090.1511097732774219RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG361970.146605925200966RNA PCR Primer, Index 1 (100% over 29bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC361880.14656947319315294RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG360040.14582423214453075RNA PCR Primer, Index 1 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC357820.14492508261847573RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG352840.14290807151948737RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC348740.14124748005244878RNA PCR Primer, Index 1 (100% over 31bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC341510.13831916875813435RNA PCR Primer, Index 1 (100% over 33bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA329000.1332523396721215RNA PCR Primer, Index 1 (100% over 23bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA326430.1322114323379046RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC324050.13124747924240415RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA319710.12948968242119746RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA318040.128813295165111RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA315810.1279100954159656RNA PCR Primer, Index 1 (100% over 28bp)
TTTTATGAGTGAAACATAAGAGTCTGACATGGAATTCTCGGGTGCCAAGG312920.12673958094285792Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATATCGTATGC311560.12618875060257193RNA PCR Primer, Index 4 (98% over 50bp)
TCGCGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC310530.12577157762426713RNA PCR Primer, Index 4 (100% over 38bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC305670.12380316920236284RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA305550.12375456652527878RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA305190.1236087584940266RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA300500.12170920386465807RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG300480.1217011034184774RNA PCR Primer, Index 1 (100% over 29bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG300430.12168085230302571Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC293910.1190401068481253RNA PCR Primer, Index 1 (100% over 31bp)
GTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCCA293040.11868773743926589RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT292470.11845687472311663RNA PCR Primer, Index 4 (100% over 43bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT283620.11487242728816746RNA PCR Primer, Index 1 (100% over 30bp)
TCCCCGTACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA270940.10973674440961884RNA PCR Primer, Index 1 (100% over 32bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCA268460.108732289083215RNA PCR Primer, Index 1 (100% over 28bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA266640.10799514848077348RNA PCR Primer, Index 4 (100% over 39bp)
CACGTTCCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA266420.10790604357278602RNA PCR Primer, Index 2 (97% over 36bp)
TAGCTTATCAGACTGATGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT263410.10668692642259428RNA PCR Primer, Index 1 (100% over 30bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT258050.1045160068461731No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA251230.10175375469856254RNA PCR Primer, Index 1 (100% over 22bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT249280.10096396119594662RNA PCR Primer, Index 4 (100% over 41bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG247800.1003645281785766RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA247300.10016201702405969RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACA252.8365794E-670.025491
CACCGAG150.002226506169.991596
CGCTATT150.002227185869.986219
TCAACGC608150.069.911036
CAACGCT607850.069.90567
TATTGCA1199950.069.873761
TCTCCCA1779100.069.866081
ATTCAAC617000.069.8658144
TAGCTTA392600.069.83821
TAGCAGC416900.069.832331
CGCTGTC609350.069.8312910
AGCTTAT394450.069.827832
CCCATAA935450.069.827111
TTCAACG609700.069.818975
TCCCAAC1792000.069.817083
CCCAACC1790550.069.8127444
GTACCAG1785000.069.80710614
TACCAGT1770450.069.8036615
AACATTC653800.069.800571
ACGCTGT610000.069.791169