FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12841956
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA8558276.6643040982230435RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC5639524.391480550159181RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG3834842.9861806098697112RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3065822.387346600471143RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2440361.9003024149903645RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2403631.8717008530476198RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC2303661.7938544564395018RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1851971.4421245486279504RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1796631.399031424807872RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1546421.2041935044786012RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1454251.1324209489582429RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1380861.0752723339030286RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1297041.010001903136874RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1183940.9219312073643611RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1044240.813147156087437RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1040280.8100635136890362RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA983160.7655843081848279RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA982180.7648211845609812RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC892680.6951277515668174RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA884440.6887112835459022RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC872570.6794681433264528RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC856690.6671024258298347RNA PCR Primer, Index 13 (100% over 50bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA753550.5867875579078452RNA PCR Primer, Index 1 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC708940.5520498590713128RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT694780.5410235014043032RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC662910.516206409677778RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA653310.5087309129543818No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC646520.5034435564177295RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC623680.4856581037966491RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA612610.4770379216374826RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA595270.463535305680848RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG595160.463449648947559RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC533640.4155441741117942RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT532020.41428268403972107RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG520860.4055924190987728RNA PCR Primer, Index 2 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT497090.3870827777326133RNA PCR Primer, Index 8 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC471420.36709361097328164RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC458120.35673693322107625RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA453430.353084841592667RNA PCR Primer, Index 1 (100% over 32bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG433470.33754203798860544RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT421890.32852472006600864RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA421520.3282366019631277RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA401530.31267043743180556RNA PCR Primer, Index 1 (100% over 28bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT390570.30413591200592804RNA PCR Primer, Index 1 (100% over 30bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA388860.3028043391520731RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG365960.2849721646764714RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC353640.27537861054811275RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA335400.2611751667736597RNA PCR Primer, Index 1 (100% over 32bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG320510.24958035987664184RNA PCR Primer, Index 1 (100% over 29bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC318060.24767255081702505RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG313510.24412947684916536RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA299430.23316541498818402RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC298160.2321764690674847RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC293730.22872683880866745RNA PCR Primer, Index 1 (100% over 27bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA292390.2276833840576934RNA PCR Primer, Index 1 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC290270.22603254519794336RNA PCR Primer, Index 13 (100% over 37bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG283770.22097101095814373RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC278640.21697629239657884RNA PCR Primer, Index 1 (100% over 27bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA275270.21435208156763658RNA PCR Primer, Index 1 (100% over 32bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA270350.21052088949689596RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263840.2054515682813428No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC255110.19865353844850425RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA251160.19557768302585682RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG240150.18700422272121162RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG236480.18414640261966325RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT235250.18318860460197808RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA234420.18254228561443445RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCA233140.1815455527179816RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC233090.1815066178392139RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG227830.17741066859285298RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA221960.17283971382552626RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG213170.16599496213816647RNA PCR Primer, Index 1 (100% over 29bp)
TAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA206390.16071539257726783RNA PCR Primer, Index 1 (100% over 32bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC203330.15833257799668524RNA PCR Primer, Index 1 (100% over 33bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA202390.15760060227585268RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTATGGAATTCTCGGGTGCCAAGGAACTCCA196250.15281939916318044RNA PCR Primer, Index 1 (100% over 28bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC194910.15177594441220635RNA PCR Primer, Index 1 (100% over 33bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA193260.15049109341287262RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT192460.14986813535258958RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC190680.14848205366845985RNA PCR Primer, Index 1 (100% over 26bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA188230.14657424460884308RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA186670.14535947639129118RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA183580.142953300883448No Hit
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG183460.14285985717440552RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC181610.14141926666000101RNA PCR Primer, Index 1 (100% over 31bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT172280.1341540182819502RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA170730.13294703704015182RNA PCR Primer, Index 1 (100% over 28bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT167200.13019823459915297RNA PCR Primer, Index 8 (97% over 36bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA161300.12560391890456563RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC160200.12474735157167646RNA PCR Primer, Index 1 (100% over 33bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC160000.12459161205660571RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG157860.12292519924534862RNA PCR Primer, Index 1 (100% over 29bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA157170.12238789791835449RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA153360.11942106015625656RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG152790.11897720253830492RNA PCR Primer, Index 1 (100% over 29bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA152190.11850998399309265RNA PCR Primer, Index 1 (100% over 28bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC150100.11688250606060321RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC149780.11663332283649003RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC148030.1152706020796209RNA PCR Primer, Index 1 (100% over 27bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC145790.11352631951082842RNA PCR Primer, Index 1 (100% over 31bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA145090.11298123120808076RNA PCR Primer, Index 1 (100% over 28bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC144120.11222589455998759RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA135320.10537335589687427RNA PCR Primer, Index 13 (100% over 38bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA130180.10137085035955581RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA129620.1009347797173577RNA PCR Primer, Index 1 (100% over 22bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC129480.10082576205680818RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph