FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13444696
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC405946530.19380282008608RNA PCR Primer, Index 29 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA6560404.879545063718807RNA PCR Primer, Index 7 (100% over 35bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3972972.955046361777165RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3359552.498792088716621RNA PCR Primer, Index 2 (100% over 34bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG3023232.248641397321293RNA PCR Primer, Index 1 (100% over 29bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3022262.2479199232173044RNA PCR Primer, Index 2 (100% over 34bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2899512.1566199786146147RNA PCR Primer, Index 1 (100% over 33bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2736172.0351296898048123RNA PCR Primer, Index 1 (100% over 32bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1316260.9790180454805375RNA PCR Primer, Index 7 (100% over 35bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA1280750.9526061429726637RNA PCR Primer, Index 2 (97% over 36bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1258860.9363246294300742RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA1235160.9186968600851964RNA PCR Primer, Index 2 (97% over 36bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA1089430.8103046733076001RNA PCR Primer, Index 1 (100% over 28bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1025980.7631113414539087RNA PCR Primer, Index 1 (100% over 31bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG977030.7267029317732435RNA PCR Primer, Index 1 (100% over 29bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC949830.706471905352118RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC754360.5610837165823608RNA PCR Primer, Index 1 (100% over 26bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT746190.5550069707786625RNA PCR Primer, Index 29 (100% over 38bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC694670.5166870266162954RNA PCR Primer, Index 29 (100% over 37bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA630140.4686904040076473RNA PCR Primer, Index 29 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA590340.4390876521120299RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATATCGTATGC587520.43699017069631024RNA PCR Primer, Index 29 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC561720.4178004471056839RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC532690.39620828912754885RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG515530.38344489157657413No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC512780.38139947530237944RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGACACCAACTAATCTCGTATGC499400.37144759539375233RNA PCR Primer, Index 29 (98% over 50bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT491090.36526671930700405RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA391720.2913565319736497RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC384670.28611282843435065RNA PCR Primer, Index 1 (100% over 31bp)
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC376450.27999889324384875RNA PCR Primer, Index 29 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC356840.26541321573950055RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA334830.24904244766858247RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT320980.23874098752400202RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAACTAATCTCGTATGC304760.2266767504449338RNA PCR Primer, Index 29 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGACTAATCTCGTATGC292930.21787774152721637RNA PCR Primer, Index 29 (98% over 50bp)
TCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTC290310.2159290176587109RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA284370.2115109185064504RNA PCR Primer, Index 1 (100% over 28bp)
CAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC272850.20294248378691493RNA PCR Primer, Index 29 (100% over 37bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA269780.20065905543717757RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA263350.19587650029424244RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA260790.1939724036899012RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC244420.18179659845042237RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC241670.1797511821762277RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAGCTCGTATGC240050.17854624604379302RNA PCR Primer, Index 29 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC235730.17533308302396722RNA PCR Primer, Index 29 (98% over 50bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA235410.17509507094842458RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCTAATCTCGTATGC231430.1721347957588628RNA PCR Primer, Index 29 (98% over 50bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA224950.1673150512291241RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA216390.16094822820835816RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTGATCTCGTATGC208790.15529544141422014RNA PCR Primer, Index 29 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAACTAATCTCGTATGC208260.15490123391410263RNA PCR Primer, Index 29 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGCTAATCTCGTATGC197430.14684601273245598RNA PCR Primer, Index 29 (98% over 50bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA195280.14524686909990378RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCCG193020.14356590881638379RNA PCR Primer, Index 29 (100% over 44bp)
TTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAAC188740.1403824973060008RNA PCR Primer, Index 1 (100% over 24bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC185120.13768998570142457RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA181700.13514623164406248RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACGAATCTCGTATGC180570.13430575150230245RNA PCR Primer, Index 29 (98% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA180380.13416443183244903No Hit
GGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTAT178720.1329297441905715RNA PCR Primer, Index 29 (100% over 48bp)
GTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA176750.13146448235051206RNA PCR Primer, Index 29 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTACTCTCGTATGC174860.13005872352933825RNA PCR Primer, Index 29 (98% over 50bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC172680.12843726626470395RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC172180.12806537239666854RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGGCACCAACTAATCTCGTATGC161650.12023328753584313RNA PCR Primer, Index 29 (98% over 50bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA160380.11928867711103322RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCGGAACTCCAGTCACCAACTAATCTCGTATGCCG158090.11758540319543112RNA PCR Primer, Index 29 (97% over 34bp)
TGGAATTCTCGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCCG157330.11702012451601733RNA PCR Primer, Index 29 (100% over 40bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACAAATCTCGTATGC154310.11477388555308353RNA PCR Primer, Index 29 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGTAATCTCGTATGC152130.11315242828844922RNA PCR Primer, Index 29 (98% over 50bp)
TTCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACT150080.1116276634295041Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACACCAGTCACCAACTAATCTCGTATGC149640.11130039682563295RNA PCR Primer, Index 29 (98% over 50bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA142770.10619057507882664RNA PCR Primer, Index 1 (100% over 32bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCC142310.10584843272023407RNA PCR Primer, Index 29 (98% over 50bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC141280.10508233135208116RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph