FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275541
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA907327.113217058487339RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA446183.4979667450909067RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC262192.0555199715258077RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA246531.9327485357193537RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG201341.578467489480934RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC195701.5342509570448932RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA160001.2543697144976131RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC158331.2412772306025444RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC151631.1887504988079567RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA127711.0012222264905637RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC114270.8958551704727641RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA113300.8882505540786224RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA104730.8210633762458439RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA104130.8163594898164779RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT102880.8065597264219652RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA102320.8021694324212236RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA94520.741018908839465RNA PCR Primer, Index 1 (100% over 22bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG93690.7345118659455087Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC85420.6696766313274132RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA73130.5733253576325653RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA72460.5680726844531065RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC72000.564466371523926RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC70360.5516090819503254RNA PCR Primer, Index 1 (100% over 26bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC68300.5354590718761686RNA PCR Primer, Index 1 (100% over 33bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA61880.4851274870819519RNA PCR Primer, Index 1 (100% over 32bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA60120.4713294202224782RNA PCR Primer, Index 1 (100% over 22bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC59720.4681934959362341RNA PCR Primer, Index 1 (100% over 31bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA59460.46615514515017553RNA PCR Primer, Index 1 (100% over 32bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA58560.45909931550612637RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54330.4259369161790958No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT53220.4172347262847686RNA PCR Primer, Index 3 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG50010.3920689338876602RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT46110.3614936720967809RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC44510.34894997495180474RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC43790.3433053112365655RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG43320.3396206002002288RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG42860.3360142872710481RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG41050.32182422987579384No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC40560.31798272262514493RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC39480.309515727052286RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT39340.30841815355210067RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA38550.3022247030867687RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC36300.284585128976646RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA34210.2681999245810209RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG34100.2673375454023038RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA33490.2625552608657817RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG32740.2566754028290741RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG32690.2562834122932936Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC32550.2551858387931082RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC31750.24891399022062013RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC31010.24311253029106866RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT30480.23895743061179534RNA PCR Primer, Index 3 (100% over 34bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC28890.2264921315739753RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC28790.22570815050241425RNA PCR Primer, Index 1 (100% over 27bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG28240.22139625460882872Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG28060.2199850886800189RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA28050.2199066905728628RNA PCR Primer, Index 1 (100% over 28bp)
TCCCCGGCATCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA28010.21959309814423839RNA PCR Primer, Index 1 (100% over 32bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT27670.21692756250093098RNA PCR Primer, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA27470.21535960035780893RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG27300.21402683253615526RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA27120.21261566660734543No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA26720.2094797423211014RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT26680.20916614989247698RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC26580.20838216882091598RNA PCR Primer, Index 1 (100% over 33bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA26210.20548143885614026RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA25670.2012479410697108RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA25060.19646565653318868No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC25030.19623046221172036RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT24810.19450570385428614RNA PCR Primer, Index 1 (100% over 30bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23750.18619550449573946RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA23640.18533312531702234No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC23360.18313797831665152RNA PCR Primer, Index 1 (100% over 31bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC23050.1807076369948124No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA22710.17804210135150497RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA22520.17655253731553905RNA PCR Primer, Index 3 (100% over 36bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT22410.17569015813682193RNA PCR Primer, Index 3 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA22380.17545496381535367RNA PCR Primer, Index 3 (100% over 36bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC22350.17521976949388535RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC22310.17490617706526093RNA PCR Primer, Index 1 (100% over 33bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGG22200.1740437978865438Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA20500.16071611967000668RNA PCR Primer, Index 1 (100% over 28bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT20260.15883456509826027RNA PCR Primer, Index 3 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC20120.15773699159807486RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG20120.15773699159807486Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC19590.1535818919188015RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA18710.14668285848906465RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC17690.13868625155914235RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA17340.13594231780867883RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG17160.134531151879869RNA PCR Primer, Index 1 (100% over 29bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA17110.13413916134408851RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA16510.12943527491472245No Hit
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA16450.12896488627178584RNA PCR Primer, Index 1 (100% over 32bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC16110.12629935062847844RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC16100.12622095252132232RNA PCR Primer, Index 1 (100% over 26bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG15970.125201777128293No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA15860.1243393979495759RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA15500.12151706609195628RNA PCR Primer, Index 1 (100% over 28bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG15370.12049789069892697No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA15040.11791075316277563RNA PCR Primer, Index 1 (100% over 22bp)
TTTCACCGGCTCTGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC14870.11657798534112193RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG14870.11657798534112193RNA PCR Primer, Index 1 (100% over 29bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG14860.11649958723396582Illumina Small RNA Adapter 2 (100% over 21bp)
TCACCCTTCTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA14840.11634279101965363RNA PCR Primer, Index 3 (100% over 36bp)
TTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA14370.11265807998331687RNA PCR Primer, Index 1 (100% over 32bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC14250.11171730269744368No Hit
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG14220.11148210837597537No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT14050.11014934055432164No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG13880.10881657273266794RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC13850.10858137841119964RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC13670.10717021248238982RNA PCR Primer, Index 1 (100% over 27bp)
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA13650.10701341626807763RNA PCR Primer, Index 1 (100% over 22bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA13630.10685662005376542RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG13620.10677822194660933No Hit
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA13230.10372069576752138RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG150.002222080570.0228651
TCTTCCA207.916135E-570.0228651
ATCCGGC207.916135E-570.0228651
CACTAGA4600.070.0228651
TCCCACA150.002222080570.0228651
GCCCGCA150.002222080570.0228651
GCTCACG150.002222080570.0228651
TCCGGTT207.916135E-570.0228651
TTAATGC1750.070.022861
AATGGCG252.835668E-670.022861
CCCCACG252.835668E-670.022861
TCGAAAC252.835668E-670.022861
GCCCGGA650.070.022861
AGGTCAG401.3460522E-1070.006395
GCAAGAT207.925364E-570.006393
CTCGTCC1200.070.006398
GGATGTA800.070.006393
CACATAA650.070.006397
GCACGGT207.925364E-570.006395
GGTCAGC401.3460522E-1070.006396