FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006134992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608713
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA2500182.6019925873527496RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2471972.5726338168285388RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2379652.4765543522842237RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1945602.024828923498912RNA PCR Primer, Index 1 (100% over 22bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1745121.8161849562995585RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1605001.6703589752342485RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1465641.5253239429671799RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1369411.4251752550003316RNA PCR Primer, Index 1 (100% over 32bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1086581.1308278226230715RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1057721.1007925827319434RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1015671.057030218302909RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG709590.7384859970320687RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA654940.6816105341058684RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG627110.6526472379807785RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG584220.6080106669852664Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC582850.6065848777042253RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC543600.5657365351634501RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC527510.5489913165269896RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA496630.5168538179879033RNA PCR Primer, Index 1 (100% over 23bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC494780.5149284820974463RNA PCR Primer, Index 1 (100% over 27bp)
AACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC486910.5067379991472323RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC480930.5005144809715932RNA PCR Primer, Index 1 (100% over 31bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA454340.47284168025416096RNA PCR Primer, Index 5 (100% over 36bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC446910.4651091150292448RNA PCR Primer, Index 1 (100% over 31bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA437040.45483718787313143RNA PCR Primer, Index 1 (100% over 22bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT423280.4405168517365437RNA PCR Primer, Index 1 (100% over 25bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC408530.4251662007180358RNA PCR Primer, Index 1 (100% over 26bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT404320.42078476066461756RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA404240.42070150289638164No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA389360.4052155580044903RNA PCR Primer, Index 1 (100% over 32bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG370790.3858893485527146Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA368570.3835789454841663RNA PCR Primer, Index 1 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG360370.3750450242399788Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC336020.349703441033154RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG331210.34469756771796595RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT317810.33075189153844015RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA313960.32674511144208385RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA312460.32518402828765935RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC297360.30946912453311903RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG287480.29918679015597616RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA278590.2899347706607534RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC274870.2860632844377806RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA274850.2860424699957216RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT274850.2860424699957216RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA273190.28431487130482513RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA272030.2831076336654035RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA271480.28253523650878115RNA PCR Primer, Index 1 (100% over 28bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG258410.2689329986232287No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT251720.26197056775449534RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC248510.2586298498040268RNA PCR Primer, Index 1 (100% over 26bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA246830.25688143667107133RNA PCR Primer, Index 5 (100% over 36bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC242730.25261447604897763RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG240920.2507307690426387RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC238680.2483995515320314RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA231070.24047965632858428RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC225390.23456835478383006RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG218000.22687741844303186RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC210810.2193946265228236RNA PCR Primer, Index 1 (100% over 27bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG207080.21551273307882124Illumina Small RNA Adapter 2 (100% over 21bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG206550.21496115036425795RNA PCR Primer, Index 5 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC203490.2117765407292319RNA PCR Primer, Index 5 (100% over 35bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG196420.20441863546137762RNA PCR Primer, Index 1 (100% over 29bp)
TTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAG188210.19587430699616065RNA PCR Primer, Index 1 (100% over 29bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC185580.19313720786540298RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA185080.19261684681392816RNA PCR Primer, Index 1 (100% over 32bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA180950.18831866452874593RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA178740.18601866868122713RNA PCR Primer, Index 1 (100% over 23bp)
TCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAAC176520.1837082656126788RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG175520.18266754350972914RNA PCR Primer, Index 1 (100% over 29bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA173780.18085668705059668RNA PCR Primer, Index 1 (100% over 23bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA172110.17911868113867072RNA PCR Primer, Index 1 (100% over 22bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC171620.17860872730822536RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT169540.17644402533409RNA PCR Primer, Index 1 (100% over 30bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA169000.17588203539849717RNA PCR Primer, Index 1 (100% over 34bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA167900.17473724108525251RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA167130.17393588506598126RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT167060.17386303451877477RNA PCR Primer, Index 1 (100% over 30bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA161850.16844087236240693RNA PCR Primer, Index 1 (100% over 28bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA160540.1670775264075428RNA PCR Primer, Index 1 (100% over 22bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA155900.16224857584985627RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG155310.16163454980911596RNA PCR Primer, Index 1 (100% over 29bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA152400.15860604848953236RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG151340.1575028830604057RNA PCR Primer, Index 5 (100% over 37bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC148930.15499474279229694RNA PCR Primer, Index 1 (100% over 33bp)
TTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAA146400.15236171587183425RNA PCR Primer, Index 1 (100% over 23bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG146180.1521327570091853Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG145840.1517789114941824RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC144760.15065493162299676RNA PCR Primer, Index 5 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC144260.1501345705715219RNA PCR Primer, Index 5 (100% over 50bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA142630.1484381935437139RNA PCR Primer, Index 1 (100% over 23bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG141290.14704362592576134Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG139650.14533684167692384Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG139330.14500381060397993No Hit
CTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC137820.14343232022852592RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC134990.1404870766771783RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA134690.1401748600462934RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC130470.1357830127718457RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC129230.1344925173641881RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC125430.1305377733729793RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT121460.12640610662426902RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG120420.12532375563720138RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC120360.1252613123110244RNA PCR Primer, Index 5 (100% over 35bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA119710.12458484294410707RNA PCR Primer, Index 1 (100% over 28bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT116120.1208486505945177No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC114120.11876720638861833RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC112200.11676901995095494RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA111840.11639435999389305RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG111460.11599888559477216Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC109060.11350115254769291RNA PCR Primer, Index 1 (100% over 27bp)
TTTTATGAGTGAAACATAAGAGTCTGACATGGAATTCTCGGGTGCCAAGG107540.11191925495120938Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC106720.11106586282679065RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA106350.11068079564869925RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG103710.10793328929691208RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC103600.10781880986558762RNA PCR Primer, Index 1 (100% over 27bp)
CTGCTGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG102860.10704867550940485RNA PCR Primer, Index 5 (100% over 37bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA102640.10681971664675592RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC102550.10672605165749044RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC102060.1062160978270451RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA101090.10520659738718391RNA PCR Primer, Index 1 (100% over 34bp)
CAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACT100410.10449890635717812RNA PCR Primer, Index 1 (100% over 25bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG99380.10342696259113993RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph