FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006134997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6160679
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1862183.022686298052536RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1207891.9606442731393732RNA PCR Primer, Index 1 (100% over 32bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA998401.6206005863964021RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA936421.5199947927817696RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA823711.3370441797081134RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA790081.2824560409656143RNA PCR Primer, Index 1 (100% over 32bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC726531.1793018269577105RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA691841.1229930986503274RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG689531.1192435119570423Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC643431.0444140978616157RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA574410.932380992419829RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC545290.8851134753166007No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT483170.7842804340235873RNA PCR Primer, Index 1 (100% over 30bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG476480.7734212413923856Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC416190.6755586518953511RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG388820.631131730771884No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA387050.6282586708380684RNA PCR Primer, Index 1 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG377960.6135038037203366RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA371680.6033101221472503RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC365660.5935384719768714RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA365600.5934410801147081No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA351880.5711708076333794RNA PCR Primer, Index 1 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA348950.5664148383644075RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC340110.5520657706723561RNA PCR Primer, Index 1 (100% over 27bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA328040.5324737744005166RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG301570.4895077312094982RNA PCR Primer, Index 15 (100% over 36bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC277610.4506159142523089RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA275320.4468987915130783RNA PCR Primer, Index 1 (100% over 22bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC274810.4460709606846908No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC267610.4343839372251013RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC247130.40114084827338026RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC235920.3829448020258806RNA PCR Primer, Index 26 (100% over 39bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA232690.3777018734460925RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA222790.3616322161891571No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC215720.3501562084309213RNA PCR Primer, Index 1 (100% over 33bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT214930.3488738822457719No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA207480.3367810593605023RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA198250.32179894456438973RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG196830.31949400382652626RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC195440.3172377590197444RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC182230.29579531736680326RNA PCR Primer, Index 1 (100% over 27bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG180970.29375008826137505No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA179600.29152630740864766RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA169870.2757325937611747RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG169680.27542418619765774RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT166370.2700514018016521No Hit
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG162890.2644026737961838Illumina Small RNA Adapter 2 (100% over 21bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC162510.26378585866914994RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA160640.26075047896506215No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC160100.25987395220559295No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG157130.2550530550285123Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA145710.23651613726344126RNA PCR Primer, Index 1 (100% over 32bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA135070.21924531370649242No Hit
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC134090.21765457995782606RNA PCR Primer, Index 1 (100% over 33bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA129550.21028526238747383RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA125590.20385739948469966RNA PCR Primer, Index 1 (97% over 37bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG125150.2031431924955025RNA PCR Primer, Index 26 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC124810.20259130527657745No Hit
TGTTGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC123880.20108173141304714No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC123830.20100057152791112No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG122860.199426069756272RNA PCR Primer, Index 1 (100% over 29bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA121550.19729968076570784RNA PCR Primer, Index 1 (100% over 22bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT121410.1970724330873269No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT117880.19134254519672264RNA PCR Primer, Index 1 (100% over 35bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC116860.18968688353994745RNA PCR Primer, Index 1 (100% over 27bp)
TTTCACCGGCTCTGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC115760.18790136606695462RNA PCR Primer, Index 1 (100% over 33bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG115140.18689498349126776Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG113050.1835025002925814Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT109400.17757782867765065No Hit
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG107760.1749157844451886No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA106100.17222127625867212RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT103670.1682769058410607No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT101240.16433253542344925RNA PCR Primer, Index 1 (100% over 35bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG98950.16061541268421875RNA PCR Primer, Index 15 (100% over 36bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT98780.1603394690747562RNA PCR Primer, Index 1 (100% over 30bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG97560.15835916787743687Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG96750.15704437773823307RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC96540.1567035062206617RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA96280.15628147481795432RNA PCR Primer, Index 1 (100% over 28bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGAA92520.15017825145572428RNA PCR Primer, Index 1 (100% over 23bp)
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG89420.14514633857728992No Hit
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCC84000.13634860702854346RNA PCR Primer, Index 1 (100% over 27bp)
GTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAGG83750.13594280760286326Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAAG83400.135374688406911No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC83020.1347578732798771No Hit
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA82490.1338975784974351RNA PCR Primer, Index 1 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC82230.1334755470947277RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCGGCATCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA81760.13271264417444895RNA PCR Primer, Index 1 (100% over 32bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG81600.13245293254201362No Hit
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA80650.1309108947244289RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG80440.13057002320685757RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG77850.1263659411568108RNA PCR Primer, Index 1 (100% over 29bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA77490.12578158998383132RNA PCR Primer, Index 1 (100% over 23bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG75530.12260012248649864No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG74870.12152881200270295No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA73150.11873691195402325RNA PCR Primer, Index 1 (100% over 28bp)
GTTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGA72570.11779545728644522RNA PCR Primer, Index 1 (100% over 22bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG72000.11687023459589438No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC71870.11665921889454067RNA PCR Primer, Index 1 (100% over 31bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG71210.11558790841074498RNA PCR Primer, Index 1 (100% over 29bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC70370.11422442234045956RNA PCR Primer, Index 26 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA69610.11299079208639178No Hit
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTC67480.10953338097959657RNA PCR Primer, Index 1 (100% over 26bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGG67010.1087704780593178Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC66180.10742322396605959RNA PCR Primer, Index 26 (100% over 50bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA65320.10602727394171974RNA PCR Primer, Index 1 (100% over 28bp)
GTTGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCCA64630.10490726752684242No Hit
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGGGTGCCAA64210.1042255244916997No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC63940.1037872611119651RNA PCR Primer, Index 1 (100% over 33bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG63170.10253739888087011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC140600.069.898396
CAACGCT140800.069.845417
ATTCAAC140650.069.7744
TATTGCA345850.069.7552951
TTCAACG140550.069.674235
GTACCAG321550.069.548314
TAATCCA76900.069.5394447
TACCAGT320250.069.52350615
ACGTAAA41950.069.494568
CATTCAA141500.069.478543
TTGTACC322550.069.47430412
CCAGTGT219150.069.4185217
CTTGTAC323450.069.4108311
TTAATGC27800.069.3988951
TCCCAAC323250.069.391633
GGTAACT87000.069.3900217
CCCAACC323650.069.381574
CGCTGTC141750.069.3778810
TGTACCA323200.069.34540613
CCCTTGT323950.069.314519
Produced by FastQC (version 0.11.5)