FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712231
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA5175049.059577597614663RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2327144.073959894128931RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1249572.1875340825677396RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1195132.092229813535202RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA956761.6749322637687445RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT946841.6575660193013904RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA772931.3531140459830844RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC704611.2335110397321116RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG670191.1732543729411504RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA568460.9951628356766384RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA542660.9499965950256564RNA PCR Primer, Index 1 (100% over 23bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA531610.9306521392429682RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC528970.9260304774089144RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA526830.9222841303161585RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA517770.9064234272038368RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC513070.8981954686356347RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG503460.881371919307885RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC452080.7914245764920922RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC445990.7807632429430812RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA410650.7188959970281313RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA395410.6922164037133652RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA345980.6056827883886349RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA339300.5939885834448922RNA PCR Primer, Index 1 (100% over 32bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG314010.5497151638300342Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC276390.48385648269476494RNA PCR Primer, Index 6 (100% over 36bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG275540.48236844763455816RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA267490.4682758802996587RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG262180.4589800377470729RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT258590.4526952779045525RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC254130.4448874704121734RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT244640.42827399662233545RNA PCR Primer, Index 6 (100% over 39bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC228560.40012387454218845RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA214380.37529994847897435RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC200700.35135133715705824RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA192310.33666355579807605RNA PCR Primer, Index 6 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC175460.3071654490163301RNA PCR Primer, Index 1 (100% over 27bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG175420.3070954238370262Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG171500.3002329562652491No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA171240.2997777925997741No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171210.29972527371529617No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC165500.28972917936967185RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC153820.26928182701294817RNA PCR Primer, Index 1 (100% over 33bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA151460.26515034143402116RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC151340.26494026589610964RNA PCR Primer, Index 5 (97% over 35bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC140250.2455257849341177RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC138550.24254971481370413RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG137240.24025639019150313RNA PCR Primer, Index 1 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC133640.23395412405415678RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA130840.22905236150288744RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG124880.21861860978661402RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA123840.21679795512471395RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG121430.2125789380716571RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC120800.2114760414976215RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG113300.19834632037814998RNA PCR Primer, Index 1 (100% over 29bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG112680.19726093009894033Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA109120.19102868914089782RNA PCR Primer, Index 1 (100% over 28bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC108880.19060853806507475RNA PCR Primer, Index 1 (100% over 33bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA108790.1904509814116411RNA PCR Primer, Index 6 (100% over 38bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA104230.1824681109710024RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG104150.1823280606123947RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC97380.1704762990152184RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC96720.1693208835567049RNA PCR Primer, Index 1 (100% over 33bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC96540.1690057702498376RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC95760.16764027925341254RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC93710.16405148881409032RNA PCR Primer, Index 1 (100% over 26bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC91080.1594473332748623RNA PCR Primer, Index 6 (100% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC90720.1588171066611277RNA PCR Primer, Index 5 (97% over 35bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA89460.15661131351305646No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC86430.15130690618078996RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA83830.14675526952603984RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC82750.14486458968483593RNA PCR Primer, Index 1 (100% over 27bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA81190.14213360769198585No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC81040.14187101326959642No Hit
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA77830.1362514926304626RNA PCR Primer, Index 1 (100% over 32bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA76990.1347809638650818RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT76980.13476345757025582RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC76490.13390564912378367RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG76430.1338006113548279RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA76360.13367806729104617RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC76260.13350300434278656RNA PCR Primer, Index 1 (100% over 26bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA76010.1330653469721375RNA PCR Primer, Index 1 (100% over 22bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA75820.13273272737044423RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC73800.12919645581559988RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA73740.12909141804664412RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG73700.1290213928673403RNA PCR Primer, Index 6 (100% over 34bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC73120.12800602776743447RNA PCR Primer, Index 1 (100% over 26bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA73110.1279885214726085RNA PCR Primer, Index 1 (100% over 28bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA71900.12587025979866712RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG69730.12207139382143335RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA69570.12179129310421796RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA68770.12039078951814099No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT67120.11750225087185724RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT66200.11589167174786874RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT66070.11566408991513122RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG65980.11550653326169756RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA65820.11522643254448217RNA PCR Primer, Index 1 (100% over 28bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG65080.11393096672736099RNA PCR Primer, Index 6 (100% over 34bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC64950.11370338489462349RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC63780.11165514839998592RNA PCR Primer, Index 5 (97% over 35bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG62910.11013210075012723Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG61150.10705099286075792RNA PCR Primer, Index 1 (100% over 29bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT59700.1045125801109934No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC59420.10402240385586647RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC59100.1034622024214357RNA PCR Primer, Index 1 (100% over 31bp)
TTTCACCGGCTCTGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC59010.10330464576800204RNA PCR Primer, Index 1 (100% over 33bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC58960.10321711429387222RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA58070.10165905405436158RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC57800.10118638409406062RNA PCR Primer, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC2050.070.019991
GTACCGA150.002222840770.019981
TTAATGC11200.070.019981
TCTTCCA353.7089194E-970.019981
CTATACG353.7089194E-970.019981
TATTGCG401.3460522E-1070.019981
ACGTACG207.9201054E-570.019981
TATACGA353.7143764E-970.00652
ATTATTA150.002224539270.006492
AGTGCAT550.070.005273
ACAACGC150.002224693870.0052646
CCCACGC150.002224693870.0052645
TTCGATA207.928352E-570.0052643
GATCGGT150.002225311770.000378
TTCGTAA1350.070.000379
CTGCGGT150.002225311770.000378
GTTGTCC150.002225311770.000378
TACCATT150.00222538969.99975615
CAGCATA150.00222538969.99975612
TGTATCA353.7180143E-969.99975613