FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006135020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27821455
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT7892442.8368178443578884RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA7609712.73519483434637RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA6990752.5127190508188733RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA5414491.9461563027526778RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC4893091.7587469814213528RNA PCR Primer, Index 1 (100% over 26bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA4344721.5616437026747882RNA PCR Primer, Index 1 (100% over 22bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG3008861.0814890881875157RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2946761.0591681851290666RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2872251.0323866958072465RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2710910.9743954800351025RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2306350.8289825244581925RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2245770.8072079623441694RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2123000.7630801480368299RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2100220.7548922225670799RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2037990.7325245929804893RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC2003680.7201923838994042RNA PCR Primer, Index 30 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC1936620.6960886840749343RNA PCR Primer, Index 31 (100% over 50bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1925080.6919408061152804RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1847620.664098984039476RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1820770.6544481588040597RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1800540.6471767921555505RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG1795300.6452933536366089Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1732510.6227244405441771RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1729880.6217791269363878RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1693190.6085914629554781RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1591050.5718787892293915RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1488360.5349684263457825RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1348420.48466911597542256RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1283530.4613453897360868RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1281130.460482746139625RNA PCR Primer, Index 2 (100% over 34bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1194300.4292730196893009RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1168340.4199420914542392No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1152380.41420551153776825RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA1123640.4038753544701382RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1106440.39769307536216203RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1058860.380591166062307RNA PCR Primer, Index 1 (100% over 26bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA961080.34544562820312597RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA956010.34362329360560045RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC932320.33510828243885876RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC886840.31876118628590777RNA PCR Primer, Index 2 (100% over 34bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA881850.31696760647493094RNA PCR Primer, Index 1 (100% over 28bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG860390.30925413498323506Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC854910.3072844321046473RNA PCR Primer, Index 30 (100% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA834330.2998872632649874RNA PCR Primer, Index 7 (100% over 35bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG762310.27400076667449635No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC731630.26297330603305974RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC729120.2620711246050934RNA PCR Primer, Index 1 (100% over 33bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG727970.2616577745484555Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG727820.26160385932367664RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG720170.2588541828599547RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG708150.2545337761810085RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG699000.2512449474694979RNA PCR Primer, Index 1 (100% over 29bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA684210.2459289063063021RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT663230.23838796353389857RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG658210.23658360067796597RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC636460.22876589308503098RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC607420.21832790556784326RNA PCR Primer, Index 30 (100% over 36bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC606840.218119433365365RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG597160.21464010419296906RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG586920.21095949151473206Illumina Small RNA Adapter 2 (100% over 21bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA577330.20751251147720348RNA PCR Primer, Index 1 (100% over 23bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT575680.206919444004636RNA PCR Primer, Index 31 (100% over 39bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA564880.20303754782055788RNA PCR Primer, Index 7 (100% over 35bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC547310.19672227782479385RNA PCR Primer, Index 1 (100% over 31bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT543800.19546066156496847RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA538720.19363473261912434RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC538640.19360597783257563RNA PCR Primer, Index 1 (100% over 33bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA533910.19190585107788216RNA PCR Primer, Index 1 (100% over 22bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA530870.19081316918903055RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG528410.1899289595026572RNA PCR Primer, Index 1 (100% over 29bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG527200.1894940433561077Illumina Small RNA Adapter 2 (100% over 21bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA516870.1857810815430034RNA PCR Primer, Index 7 (100% over 35bp)
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA483060.17362858987784788RNA PCR Primer, Index 7 (100% over 35bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT482540.17344168376528113RNA PCR Primer, Index 31 (100% over 39bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC479690.17241729449448276RNA PCR Primer, Index 1 (100% over 26bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG461680.1659438731727007RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT460570.16554490050933712RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA459800.16526813568880563RNA PCR Primer, Index 1 (100% over 32bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA454920.1635140937093333RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC454440.16334156499004096RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC442160.15892770525481142RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC438930.15776673074790662RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT434150.1560486322516202RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA430960.15490203513798972RNA PCR Primer, Index 1 (100% over 23bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG415010.14916904956983737RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC408390.14678959098293026RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG404720.14547046515000744RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA401790.1444173210926603RNA PCR Primer, Index 1 (100% over 32bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA400600.14398959364274802RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTCGGGTGCCAAGGAA398590.14326712963071128RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC394050.14163529549407103RNA PCR Primer, Index 1 (100% over 27bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG390440.14033773575105973Illumina Small RNA Adapter 2 (100% over 21bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA378010.13586996079105138RNA PCR Primer, Index 1 (100% over 32bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA373680.13431360796910155RNA PCR Primer, Index 1 (100% over 23bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG368530.13246251858502728Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT364290.13093851489794475RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC359440.12919525596342823RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG342960.12327176993439057RNA PCR Primer, Index 31 (100% over 37bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC342450.12308845817014243RNA PCR Primer, Index 1 (100% over 27bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG339800.12213595586571588No Hit
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG337530.12132003879739574RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG335680.12065508435845644RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC331810.11926407155916181RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC327470.1177041243888934RNA PCR Primer, Index 1 (100% over 26bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG325540.11701041516340535Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA325130.11686304688234314RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG317500.11412055911525834RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT310170.11148590179773127RNA PCR Primer, Index 1 (100% over 30bp)
TTGCAGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG309380.11120194828056261RNA PCR Primer, Index 31 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC307300.11045432383029571RNA PCR Primer, Index 1 (100% over 27bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT306390.11012723813330395No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA301840.10849180964834512No Hit
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC297850.10705766466922739RNA PCR Primer, Index 2 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT297260.10684559811843056RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT296940.10673057897223565RNA PCR Primer, Index 1 (100% over 30bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA294920.10600452061188029RNA PCR Primer, Index 1 (100% over 32bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT292430.10510952788055118RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT291140.10464585694745297RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA290880.10455240389116961RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA279620.10050516768443635RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph