FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8063395
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2999873.720356003891661RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2780713.4485598187860074RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2254322.7957454645344795RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2226942.7617895439824047RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1851062.295633538974588RNA PCR Primer, Index 4 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1551211.9237678422054234RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1537011.9061573940009142RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1106921.3727716427137702RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA997641.2372456018835738RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA936711.1616818970173233RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC928301.1512520470595822RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC916491.136605610912029RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA887171.1002437558869433RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT853531.058524356055979RNA PCR Primer, Index 3 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC849011.0529187767683463RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA763570.9469584461631856RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC761670.944602118586526RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC729530.9047429773687138RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC625880.7761991071998829RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA593740.7363399659820709RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA571770.709093378161432RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA530190.6575270094048475RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG506280.6278744871111982RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA481350.5969569889606053RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC477780.5925295734613025RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA443170.5496072063938329RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC399740.4957465186810271RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC377860.4686115463771774RNA PCR Primer, Index 1 (100% over 26bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA377060.46761940845016275RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC349570.43352706893312304RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC348800.43257213617837154RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC345300.4282315327476826RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC322110.3994719345883465RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA309500.38383336051377864RNA PCR Primer, Index 1 (100% over 32bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA303170.3759830691662755RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG296730.3679963588538079RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA292090.36224195887712307RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC277930.34468111756896447RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG275620.34181631930470974RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA270830.33587589346670976RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA264690.3282612348768726RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC252290.3128830970081461RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA246040.3051320194533444RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT243730.30226722118908966RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC227870.282598086786025RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT226150.2804649902429436RNA PCR Primer, Index 1 (100% over 25bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC219920.2727387161363173RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT209230.2594812730865845RNA PCR Primer, Index 3 (100% over 34bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA206440.256021192066121RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC203790.25273473518288514RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC202830.2515441696704676RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT200800.249026619680668RNA PCR Primer, Index 3 (100% over 34bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA197400.24481003349085592RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT197300.2446860162499791RNA PCR Primer, Index 1 (100% over 30bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA195660.2426521334995991RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA194390.2410771145404634RNA PCR Primer, Index 2 (97% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC192170.23832393179299785RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA181170.22468203529654696RNA PCR Primer, Index 2 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG177950.22068868014031312RNA PCR Primer, Index 2 (97% over 35bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT176960.2194609094556325RNA PCR Primer, Index 1 (100% over 30bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA175480.2176254542906555RNA PCR Primer, Index 1 (100% over 32bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG174010.21580240084976615No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC162890.202011683664263RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT156790.19444663197077658RNA PCR Primer, Index 1 (100% over 30bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA155830.19325606645835905RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG154250.19129659405250518RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC153000.18974637854154486RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC149520.18543057855903128RNA PCR Primer, Index 1 (100% over 33bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT149080.18488490269917324No Hit
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC148340.18396717511668473RNA PCR Primer, Index 1 (100% over 33bp)
AACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC143840.17838639927722752RNA PCR Primer, Index 1 (100% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA143630.1781259630713862RNA PCR Primer, Index 1 (100% over 22bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA140520.17426902688011686RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC140130.17378535964069725RNA PCR Primer, Index 1 (100% over 33bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC138570.17185069068301875RNA PCR Primer, Index 1 (100% over 31bp)
ATCTCGGTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA137460.17047409930928598RNA PCR Primer, Index 1 (100% over 32bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA136760.1696059786231482RNA PCR Primer, Index 1 (100% over 28bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT133700.1658110510523173RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC131330.1628718424435365RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA130550.16190450796469724RNA PCR Primer, Index 1 (100% over 32bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG124540.15445107178799997RNA PCR Primer, Index 2 (97% over 35bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC123620.15331011317193316RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA118800.1473324821616701RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC118390.14682401147407512RNA PCR Primer, Index 1 (100% over 27bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT117330.14550942872078076RNA PCR Primer, Index 1 (100% over 30bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA117100.14522418906676407RNA PCR Primer, Index 4 (100% over 39bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG117060.14517458217041332RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC116560.1445544959660292RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC115740.1435375545908392RNA PCR Primer, Index 1 (100% over 27bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT111410.1381676080608726RNA PCR Primer, Index 3 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC108680.13478193738493527RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG108260.13426106497325258RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG107640.13349215807981626RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG103210.12799819430897283RNA PCR Primer, Index 1 (100% over 29bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA102550.12717968051918577RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA102470.12708046672648432No Hit
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC100930.12517060121698118RNA PCR Primer, Index 1 (100% over 27bp)
ATCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA99850.12383121501551146RNA PCR Primer, Index 1 (100% over 32bp)
CCCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC99640.12357077880967011RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC98100.12166091330016698RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT97800.1212888615775365RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC97250.12060676675271396RNA PCR Primer, Index 1 (100% over 27bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT95070.11790319090159915RNA PCR Primer, Index 3 (100% over 34bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC94620.11734511331765343RNA PCR Primer, Index 1 (100% over 33bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA93050.11539804263588724RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC92600.11483996505194152RNA PCR Primer, Index 1 (100% over 26bp)
CTCCTGGCTGGCTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA92180.11431909264025884RNA PCR Primer, Index 1 (100% over 32bp)
TCCCCGTACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA92090.11420747712346971RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC91470.11343857023003338RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG90940.11278127885338621RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCC89290.11073499437891855RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG89200.11062337886212942RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG87720.10878792369715237RNA PCR Primer, Index 2 (97% over 35bp)
TCGTTTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA87470.1084778805949603RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC87220.10816783749276825RNA PCR Primer, Index 4 (100% over 37bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA82260.10201658234527763RNA PCR Primer, Index 1 (100% over 28bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG81710.1013344875204551RNA PCR Primer, Index 1 (100% over 29bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC81620.10122287200366595RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph