FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13287382
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA8805976.6273175558586335RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC6234514.692052956707348RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA3964302.9835072100734368No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC2816022.1193189147418203RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2800712.1077967051748794RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2616511.969168945394962RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1922781.44707211699039RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1671491.2579528457900886RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1503591.1315923633414016RNA PCR Primer, Index 1 (100% over 31bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1502051.130433369041396RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA1443121.0860830222236404RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1397861.05202063130269RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1360521.0239187824960554RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1338071.0070230539018146RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1292310.9725843661302129RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1249820.9406066597618704RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1227110.9235152568052909RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1100270.8280562717320838RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC937420.7054963874749745RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA933010.7021774492522304RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA920080.6924464126943893RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA852500.6415861303603675No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC833030.626933130996008RNA PCR Primer, Index 1 (100% over 31bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA827010.6224025169141671RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC770210.579655194680186RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC738430.5557377668527932RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA735850.5537960751034328RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA733840.5522833617638148RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC720260.542063139300127RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA655290.49316712652650463RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT644550.48508427017451594RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC588900.44320243069703275RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT581600.43770849667752454RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC568240.4276538448281234RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA562560.4233791126047253RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG560340.4217083545878338RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC533860.401779673377344RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA513360.3863515025006431RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG498430.37511527854019705RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC468480.3525750971861876RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC463280.3486616099394147RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA440520.3315325772977702RNA PCR Primer, Index 1 (100% over 32bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG425600.3203038792743371RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT418490.31495293805807645RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG389410.2930675132242002RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA388590.2924503863891322RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC386080.2905613761988629RNA PCR Primer, Index 1 (100% over 27bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG373640.28119911055466Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA361310.27191963021760046RNA PCR Primer, Index 1 (100% over 22bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA355890.26784057235654096RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC351650.26464957506301845RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC344550.2593061597837708RNA PCR Primer, Index 1 (100% over 31bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA343860.2587868701298721RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC336890.25354129203179376No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC313550.2359757550433938RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA309610.23301053586026202RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA288000.21674698597511535RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA274750.20677511943285742RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC274640.20669233412571414RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC273690.2059773701094768RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT269000.20244770565036813RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262370.19745800941073266No Hit
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC258130.19426701211721015RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC244900.18431019744897828RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG241470.18172880105351077RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC235890.17752932820024292RNA PCR Primer, Index 2 (100% over 50bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA235500.17723581665673493RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA232850.17524144334828334RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC232470.17495545774178842RNA PCR Primer, Index 1 (100% over 27bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC230860.1737437818826914RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA230710.17363089282749603RNA PCR Primer, Index 2 (100% over 36bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA224750.16914543436773324RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA221400.16662424546836993RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG218660.16456213872680112RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA218490.16443419779757967RNA PCR Primer, Index 1 (100% over 32bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA215950.16252260979627137RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG215770.16238714293003695RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG215500.16218394263068525RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC209430.15761569886377919RNA PCR Primer, Index 2 (100% over 34bp)
AACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC206300.15526008057870241RNA PCR Primer, Index 1 (100% over 31bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG200680.15103050397738246RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG194350.14626658584813773RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC190350.1432562110429278RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG189150.14235309860136483RNA PCR Primer, Index 1 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA186460.14032862154486114No Hit
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT183700.13825146292926627RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTTGGAATTCTCGGGTGCCAAGGAACT183660.13822135918121417RNA PCR Primer, Index 1 (100% over 25bp)
ACCACAGGGTAGAACCACGGACTTGGAATTCTCGGGTGCCAAGGAACTCC177490.13357785604417785RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTACATGGAATTCTCGGGTGCCAAGGAACTCC177220.13337465574482618RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA175930.132403809870146No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG174320.13119213401104898Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA169850.12782804016622687RNA PCR Primer, Index 1 (100% over 32bp)
TGTCAGTTTGTCAAATACCCCAGTGGAATTCTCGGGTGCCAAGGAACTCC169610.1276474176779143RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA169280.12739906175648447RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC168130.1265335789999866RNA PCR Primer, Index 1 (100% over 24bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT165310.1244112647623136RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA165040.12420806446296193RNA PCR Primer, Index 2 (100% over 36bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA162550.12233410614671875RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA162030.12194275742204147RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA159390.11995591005060288RNA PCR Primer, Index 1 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA159340.11991828036553777RNA PCR Primer, Index 1 (100% over 23bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT158070.1189624863648836RNA PCR Primer, Index 1 (100% over 30bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCA155560.11707347617461439RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC155210.1168100683791585RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC153620.11561344439408756RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC153430.1154704515908401RNA PCR Primer, Index 1 (100% over 33bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC151750.11420609417265191RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA151060.1136868045187532RNA PCR Primer, Index 1 (100% over 23bp)
AGCTCGGTCTGAGGCCCCTCAGTTTGGAATTCTCGGGTGCCAAGGAACTC150990.11363412295966202RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCGTACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA150180.11302452206160701RNA PCR Primer, Index 1 (100% over 32bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG147000.11063127409146511RNA PCR Primer, Index 1 (100% over 29bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC146420.11019476974470968RNA PCR Primer, Index 1 (100% over 27bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA140520.10575446690702503RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC140420.10567920753689478No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC140420.10567920753689478RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC138190.10400092358299025RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAAC137360.10337627081090917RNA PCR Primer, Index 1 (100% over 24bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC136820.10296987021220583RNA PCR Primer, Index 1 (100% over 33bp)
TCGAGCCCCAGTGGAACCACCATGGAATTCTCGGGTGCCAAGGAACTCCA134520.10123890469921012RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC134050.10088518565959795RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA133240.10027558476154294RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATACG150.002221340370.032421
CAACGGA778350.069.969441
CAACGCT311000.069.873577
TCAACGC311250.069.858556
TATTGCA883800.069.838281
TGGTCCG592450.069.82754
TTCAAGT1260450.069.812951
TGAGGTA344750.069.7988051
CAGCACG316600.069.794684
ATTCAAC330800.069.793124
CTGGTCC578550.069.7879943
TTCAACG311850.069.7805255
TCAAGTA1275150.069.770512
TCAGTGC298300.069.762431
ACGCTGT311450.069.750419
GGTCCGA593800.069.727965
CCCATAA402400.069.7191541
AACGCTG311900.069.7168358
AAGTAAT1281700.069.712544
GGATAGG1279300.069.7056914