FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006135043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16605503
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3793372.284405356465263RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3263361.96522803314058RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3147651.8955463137732114RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3063461.844846253678675RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA2974211.7910990109724472RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2637331.5882265053940252RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2364531.4239436167636716RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG2323221.3990663215682173RNA PCR Primer, Index 7 (100% over 36bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1701061.0243953465306048RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1543300.9293906965660721RNA PCR Primer, Index 1 (100% over 32bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1498110.9021768265616524RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1435810.8646591434176971RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1429850.8610699718039254RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1308900.7882326720244488RNA PCR Primer, Index 1 (100% over 23bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1256140.7564600723025373RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1236990.7449277507582878RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1235300.7439100158543827RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1177200.7089216147201322RNA PCR Primer, Index 1 (100% over 26bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA1024870.6171869650681463RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC997410.6006502784046951RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA968870.5834632049387483RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA948760.5713527617922806RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC938880.565402926969451RNA PCR Primer, Index 1 (100% over 33bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA921890.5551713790301926RNA PCR Primer, Index 7 (100% over 35bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG909940.54797496950258No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA857190.5162083918806916RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG835900.5033873409314972RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA810820.4882839140735454RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA803330.48377336115623837RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC769590.4634547956782761RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC747770.450314573427857RNA PCR Primer, Index 1 (100% over 27bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG683100.4113696525784254Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA666730.4015114748405995RNA PCR Primer, Index 7 (100% over 35bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA614320.3699496486194968RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG590830.3558037356652189RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC590360.3555206969641329RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG586580.35324434315539854RNA PCR Primer, Index 7 (100% over 36bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC577780.3479448951350646RNA PCR Primer, Index 7 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG569750.3431091488165098RNA PCR Primer, Index 1 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG547050.3294389817640574Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA540370.32541621894862205RNA PCR Primer, Index 1 (100% over 28bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA531230.3199120195275024RNA PCR Primer, Index 1 (100% over 22bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC523110.31502207430873974RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC522870.3148775439081852RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC521730.314191024505551RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC520370.31337201890240846RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG510520.30744025037964823RNA PCR Primer, Index 1 (100% over 29bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG498980.300490746952983Illumina Small RNA Adapter 2 (100% over 21bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG493500.29719063614032043RNA PCR Primer, Index 7 (100% over 36bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC478970.2884405248067463RNA PCR Primer, Index 2 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC460620.27738997126434534RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA402280.24225703972954024RNA PCR Primer, Index 1 (100% over 32bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA397480.23936643171844899RNA PCR Primer, Index 7 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC390950.23543400040336027RNA PCR Primer, Index 7 (100% over 50bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC381450.2297130053814088RNA PCR Primer, Index 1 (100% over 31bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG380040.22886388927815077Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC367100.22107129184825053RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC359340.2163981422303197RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA355530.21410372212151596RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG355270.2139471475209152RNA PCR Primer, Index 1 (100% over 29bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA348830.21006891510603443RNA PCR Primer, Index 1 (100% over 23bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA341760.20581129038969795RNA PCR Primer, Index 1 (100% over 32bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG338730.20398659408269654RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG337860.2034626713806863RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA332040.19995780916723813No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA321840.1938152671436692RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA312710.18831708982257267RNA PCR Primer, Index 1 (100% over 32bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG311630.18766670302007715Illumina Small RNA Adapter 2 (100% over 21bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA309350.18629366421480878RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC303960.18304775230235423RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC292810.17633311077659014RNA PCR Primer, Index 1 (100% over 33bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG292110.17591156377497266Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC289370.1742615083686414RNA PCR Primer, Index 1 (100% over 27bp)
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCC288780.1739062044672781RNA PCR Primer, Index 1 (100% over 27bp)
AGGTCGCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC288340.1736412320662614RNA PCR Primer, Index 7 (100% over 39bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG287540.17315946406441285Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA287300.17301493366385828No Hit
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA286710.172659629762495RNA PCR Primer, Index 7 (100% over 35bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT285890.17216581756060023RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT279930.16857664594682858RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC271530.16351808192741887RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA270840.16310255702582452RNA PCR Primer, Index 1 (100% over 28bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA265870.16010957331434045RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC260770.15703830230255597RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG257720.1552015617955084RNA PCR Primer, Index 1 (100% over 29bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT257550.15509918609511558No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC253900.1529011195866816RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT248070.14939023527321033RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC247920.14929990377286373RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC244720.14737283176546956RNA PCR Primer, Index 2 (100% over 34bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG244170.14704161626419868Illumina Small RNA Adapter 2 (100% over 21bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC242330.14593354985994703RNA PCR Primer, Index 2 (100% over 34bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA242220.14586730675969287RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT237870.1432476932496414RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA236880.14265150534735382RNA PCR Primer, Index 1 (100% over 32bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG235110.14158559364326392No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG231980.1397006763360315RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC230980.1390984663337208RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA222930.13425067581511985RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC218660.13167923910525323RNA PCR Primer, Index 7 (100% over 39bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA205150.12354338197403596No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC203390.12248349236996915RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA200440.12070697286315266RNA PCR Primer, Index 1 (100% over 32bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA199570.12018305016114238RNA PCR Primer, Index 7 (100% over 37bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG199070.11988194515998701RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA197710.11906293955684451RNA PCR Primer, Index 1 (100% over 32bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC192490.11591940334478275RNA PCR Primer, Index 2 (100% over 34bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA189360.11403448603755031RNA PCR Primer, Index 1 (100% over 28bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT186550.11234227593105732No Hit
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTC185060.11144498302761441RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCCTTTGGAATTCTCGGGTGCCAAGGA182950.11017431992273885RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG182580.1099515022218839RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA180960.10897592201814062RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT180870.10892172311793265RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC180550.10872901591719322RNA PCR Primer, Index 1 (100% over 26bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA179430.10805454071460528RNA PCR Primer, Index 7 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT175920.10594078360649478RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC173910.10473034150185032RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC171710.10340547949676683RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA171090.10303210929533421RNA PCR Primer, Index 1 (100% over 32bp)
AGGTCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC167410.10081597648683091RNA PCR Primer, Index 7 (100% over 39bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC166290.10014150128424294No Hit

[FAIL]Adapter Content

Adapter graph