FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11346972
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG537430.47363296569340263No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT387440.34144792108414473No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA185120.16314484604350835No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG170200.14999596368088333No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT144910.12770807930080377No Hit
GGGGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG144590.1274260657380665No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG126760.11171262253929948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG131100.0120.606014
TACGGCC133700.0117.989537
TCTACGG143350.0109.693215
GGTCTAC127500.0102.2908863
CTACGGC158100.099.687736
ACGGCCA166350.095.006538
CGGCCAT176500.089.378299
GGGTCTA134050.087.694842
CGGTCTA23300.074.479372
GCGCGGT182700.071.028459
GCGTCTA34350.070.812852
GGGGGAG635800.063.1782461
CGTCTAC47950.062.1397063
GGGGGGC559200.061.5799831
CGGGGAG298300.061.2395551
GGGGGGT314900.061.1033971
CGGGGAT86650.060.714161
GCCGGTT51050.060.0704043
CGGTTCA52450.058.6054845
CGGGGGT119750.056.9783821
Produced by FastQC (version 0.11.5)