FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10490837
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA3959983.7747035818019095RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2895572.760094356627598RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1852481.7658076281234756RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1738011.6566933601198837RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1688201.609213831079446RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1395111.3298366946317057RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1387121.3222205244443317RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1236551.1786952747430923RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1132231.079256116551997RNA PCR Primer, Index 15 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC1027230.9791687736641033RNA PCR Primer, Index 26 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA999580.952812440036958RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA997050.9504008116797545RNA PCR Primer, Index 1 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG937660.893789504116783RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA882780.841477186234044RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA880780.8395707606552271RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC852340.812461388924449RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG763340.7276254506670916RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC719720.6860463087930925RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA709700.6764951166432193RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG680550.6487089638319611No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA677480.645782600568477RNA PCR Primer, Index 1 (100% over 32bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA651740.621246903369102RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA640150.6101991671398574RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA625370.5961106821123996RNA PCR Primer, Index 1 (100% over 22bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT604480.5761980669416559RNA PCR Primer, Index 1 (100% over 35bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG583170.5558851023993605Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC523900.49938818037111815RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG479140.4567223759171932RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG422340.40257988947878987RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC407950.38886315743920147RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG395370.3768717405484424RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA383600.3656524260171043No Hit
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC383590.3656428938892102RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA376810.3591801111770205RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA374160.3566540972850879RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC367560.3503628928749918RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA361130.344233734639095RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT336380.32064171810123443RNA PCR Primer, Index 1 (100% over 35bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC323760.30861217269889907RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA321040.3060194339117079RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG316370.30156793018517014RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC311860.2972689405049378RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA301020.28693611386774953RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC300170.28612588299675235RNA PCR Primer, Index 1 (100% over 27bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG294530.28074976286448833Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG286680.27326704246763156RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC277320.26434497075876784RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTGGTATGC275800.26289608731886693RNA PCR Primer, Index 26 (98% over 50bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC274110.26128515770476657No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC270140.25750090293081473RNA PCR Primer, Index 26 (100% over 39bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA262490.25020882509183967RNA PCR Primer, Index 1 (97% over 37bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC259900.24774000396727164RNA PCR Primer, Index 1 (100% over 33bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA257640.24558574306320838RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG255700.2437365102517559RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT253640.24177289190557436RNA PCR Primer, Index 1 (100% over 30bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG250470.23875120736314936RNA PCR Primer, Index 15 (100% over 36bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC247210.23564373366967767RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT226950.21633164255626122No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT222580.21216610266654606No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC222200.2118038818065708RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC217240.20707594637110463RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG215300.2052267135596521Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA207840.19811574615066463RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA202910.19341640709888067No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG201260.19184360599635664RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA197020.18780198376926455RNA PCR Primer, Index 1 (100% over 32bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA192090.1831026447174806RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC190550.1816346970217915RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA189830.18094838381341735RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC188780.17994751038453843RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC177460.1691571416084341RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC175480.16726978028540523RNA PCR Primer, Index 1 (100% over 27bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC169260.16134079673528434No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC167540.1597012707375017RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGG164730.1570227427992638RNA PCR Primer, Index 26 (97% over 49bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA164310.1566223934277122RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA163910.1562411083119488No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC159000.151560833515953RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC157360.14999756454132307RNA PCR Primer, Index 1 (100% over 26bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA156170.14886324132192694RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC155750.14846289195037535RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGCGTATGC153330.14615611700000677RNA PCR Primer, Index 26 (98% over 50bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC147160.14027479408935628RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG144340.13758673402322424RNA PCR Primer, Index 26 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG142560.13589001525807712RNA PCR Primer, Index 1 (100% over 29bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG142350.13568984057230135No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC141990.13534668396811428RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC139580.13304944114563977RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG138660.13217248537938392RNA PCR Primer, Index 1 (100% over 29bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG138000.13154336493837432No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT135960.12959881084798097RNA PCR Primer, Index 1 (100% over 35bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG135290.12896015827907725Illumina Small RNA Adapter 2 (100% over 21bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA133840.12757799973443493RNA PCR Primer, Index 1 (100% over 34bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG133340.12710139333973067No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG132740.12652946566608556Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTGGTATGG131580.1254237388303717RNA PCR Primer, Index 26 (97% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC130760.1246421043430567No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT129710.12364123091417778RNA PCR Primer, Index 1 (100% over 30bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG129480.1234219919726138RNA PCR Primer, Index 15 (100% over 36bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATC129150.12310743175210902RNA PCR Primer, Index 26 (100% over 42bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA128130.12213515470691233RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA127050.12110568489435115RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC127000.12105802425488071RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGGGTATGC124640.11880844207187663RNA PCR Primer, Index 26 (97% over 42bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG124290.11847481759558365RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC123530.11775037587563317RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG123300.11753113693406922RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC121150.11548172943684093RNA PCR Primer, Index 1 (100% over 27bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA120440.11480494835636088No Hit
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC118830.1132702757654132RNA PCR Primer, Index 1 (100% over 31bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA118660.11310822959121375RNA PCR Primer, Index 1 (100% over 34bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT118590.11304150469595516No Hit
GGGCGGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA117260.11177373168604182RNA PCR Primer, Index 1 (97% over 37bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG117110.11163074976763056RNA PCR Primer, Index 15 (100% over 36bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA116280.11083958315242148RNA PCR Primer, Index 1 (97% over 37bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA116110.11067753697822204RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA115360.10996262738616566RNA PCR Primer, Index 1 (100% over 32bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC112870.10758912754053847RNA PCR Primer, Index 1 (100% over 33bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG112580.10731269583161No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT112540.10727456732003365RNA PCR Primer, Index 1 (100% over 30bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT111610.10638807942588374No Hit
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAT111340.10613071197274346RNA PCR Primer, Index 26 (100% over 41bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA109350.10423381852182052RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG108850.10375721212711626RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC106280.1013074552583364RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph