FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14164815
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA538590.38023087488258756No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT503370.3553664484852079No Hit
GGGGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG289530.2044008340384255No Hit
GGGGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA257070.18148489761426465No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT204330.14425179573471308No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG197920.13972649836937512No Hit
GGGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG160430.11325950956648569No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG62500.089.561024
CGGTTCA106650.084.506875
TACGGCC66650.084.41967
GCCGGTT107150.083.828833
TCTACGG73550.076.302715
CCGGTTC121550.074.267044
GGTTCAC134600.067.605256
CGGGGAT132550.064.7330861
GGTCTAC76750.063.758733
GTTCACC147650.061.3353277
CGGGGAG509400.060.1104971
CGGGGGT165650.058.821671
GCGCGGT265250.057.89659
GGGGGAG1244500.057.546811
GGGGGAT399200.057.4506761
GGGGGGT507100.055.421711
TTCACCC164900.055.138968
GGGGAGT415250.054.610412
CGGGGTG138000.053.3243331
GGGGGTT306000.052.730261
Produced by FastQC (version 0.11.5)