FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9721508
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA4082124.199060474979808RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3937084.049865514691754RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1960972.017145899586772RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1738011.7877987653767295RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1714571.7636872797923944RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1598591.644384801205739RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1346261.3848263047255631RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1289791.326738608866032RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1217681.2525628739903316RNA PCR Primer, Index 1 (100% over 30bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1145891.1787163061533252RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC893860.9194664037719251RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA863670.8884115509651384RNA PCR Primer, Index 1 (100% over 23bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA836950.8609261032341896RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG822990.8465661911711639RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA797390.8202328280756442RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA723800.7445346956459842RNA PCR Primer, Index 1 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG693360.7132226810902177Illumina Small RNA Adapter 2 (100% over 21bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA671400.6906335930598422RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC647170.665709476348731RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA645500.6639916358655468RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG591110.6080435257575265No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG547420.5631019385058367RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA535610.5509536174840364RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC507480.5220177774888423RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC483360.4972068119472823RNA PCR Primer, Index 1 (100% over 27bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA482590.49641475376042477RNA PCR Primer, Index 1 (100% over 22bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC409690.42142638775794866RNA PCR Primer, Index 1 (100% over 31bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC384410.3954221917011229RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA382910.39387922120724483RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG382130.39307687655042817RNA PCR Primer, Index 6 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC382080.39302544420063223RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG381790.39272713657181585RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG367370.3778940468906676RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA364420.3748595382527073RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA358570.36884195332658265RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC356320.3665274975857655RNA PCR Primer, Index 1 (100% over 27bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA337160.34681862114396245RNA PCR Primer, Index 1 (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC335710.3453270829998803RNA PCR Primer, Index 1 (100% over 27bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG322600.3318415208833856Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA303940.3126469679395419RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC295120.3035743014355386RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC291340.29968601579096577RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC289750.29805046706745497RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG288840.2971143983011689RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG275260.28314537209659246Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC273100.28092349458540794No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG269820.2775495324387945Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA268400.27608885370458985RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA268280.2759654160650796RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG266010.27363038738434403RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT263300.27084275402540425RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC262800.27032843052744493RNA PCR Primer, Index 1 (100% over 33bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT257320.26469144498981023No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA242670.2496217664996007No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT241910.24883999478270244RNA PCR Primer, Index 1 (100% over 30bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG240580.24747189427813052Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA232340.23899584303176008No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT232290.23894441068196415RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA225540.23200104345951267RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG214850.22100480707314135RNA PCR Primer, Index 1 (100% over 29bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG213540.21965727950848776No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA210070.21608787443264973RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC203460.2092885177896269RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC202770.20857875136244294RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG201740.20751924495664664RNA PCR Primer, Index 6 (100% over 34bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG196210.201830827069216Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG194530.20010270011607253RNA PCR Primer, Index 1 (100% over 29bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA194130.19969124131770502RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT184090.18936362547868088No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC181690.1868948726884759RNA PCR Primer, Index 1 (100% over 26bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG173190.1781513732231666No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA169480.1743350928683081No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC168710.17354303468145066No Hit
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG165360.17009706724512288Illumina Small RNA Adapter 2 (100% over 21bp)
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCC156580.16106554662095635RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA150730.1550479616948317RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC148610.15286723006348396RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA147380.15160199425850393RNA PCR Primer, Index 3 (97% over 36bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA146800.15100537900087105RNA PCR Primer, Index 1 (100% over 32bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC146690.15089222783132No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT145330.1494932679168705No Hit
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT144650.14879378795964576RNA PCR Primer, Index 1 (100% over 30bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG144130.14825889152176802No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT141950.14601644107066516RNA PCR Primer, Index 1 (100% over 25bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC139020.14300250537262327RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG137800.1417475560376024RNA PCR Primer, Index 1 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC136870.14079091433139798RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC135050.13891877679882586RNA PCR Primer, Index 1 (100% over 27bp)
TTCAGAGTTCTACAGTCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAG128350.13202684192617029Illumina DpnII expression Sequencing Primer (95% over 22bp)
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA126630.13025756909319008RNA PCR Primer, Index 1 (100% over 22bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG124780.1283545721507404Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC123930.1274802222042095RNA PCR Primer, Index 1 (100% over 33bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG122600.12611212169963756RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT121460.12493946412429019RNA PCR Primer, Index 1 (100% over 30bp)
CAGAGTTCTACAGTCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGA119740.12317019129130995RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC119460.1228821701324527RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG119270.12268672720322814RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA117520.12088659496037035RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATTGTCTGCTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG114240.11751263281375689RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC113880.11714231989522614RNA PCR Primer, Index 1 (100% over 26bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT112560.11578450586061338No Hit
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTC110900.11407695184738828RNA PCR Primer, Index 1 (100% over 26bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA110240.11339804483008191RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC107510.11058983853122376RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT107020.11008580150322358RNA PCR Primer, Index 1 (100% over 30bp)
GTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAGG106720.10977720740444795Illumina Small RNA Adapter 2 (100% over 21bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGAA104400.10739074637391648RNA PCR Primer, Index 1 (100% over 23bp)
GTTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGA104280.10726730873440622RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG103490.10645467760763042RNA PCR Primer, Index 6 (100% over 34bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA103170.10612551056893643RNA PCR Primer, Index 1 (100% over 23bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG102410.10534373885203818RNA PCR Primer, Index 1 (100% over 29bp)
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGGGTGCCAA102090.10501457181334417No Hit
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT100380.10325558545032312RNA PCR Primer, Index 1 (100% over 30bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG99180.10202120905522065RNA PCR Primer, Index 17 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph