FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12338216
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA4340593.5180045478211763RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA4050523.2829057296451936RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3260972.642983394033627RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2469752.00170754021489RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2466511.9990815527949908RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1662021.3470504974138886RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1526101.2368887041692251RNA PCR Primer, Index 1 (100% over 22bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1491471.2088214373941906RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1488241.2062035548737355RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1268061.0277498789128023RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1171320.949343081690254RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1159750.9399657130333916RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1013360.82131809007072RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA947340.7678095439405502RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG915820.7422629008926411RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC901910.7309889857658514RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC863420.6997932278053812RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG833120.6752353824896565RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA684650.5549019404426053RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC653100.5293309826963638RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA631670.5119621831875856RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC630940.5113705255281639RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG596720.4836355596303387RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA569800.46181717032673114RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC565090.45799976268854425RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA521500.4226705060115661RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG515070.41745905566898817Illumina Small RNA Adapter 2 (100% over 21bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA499070.4044912165583744RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC490150.39726164625420723RNA PCR Primer, Index 18 (100% over 50bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC489620.3968320865836682RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC482660.39119107657055124RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA426630.34577932498507075RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA392120.3178093170033658RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA381140.30891013741370715RNA PCR Primer, Index 1 (100% over 28bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA360480.2921654151621272RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA349630.2833715992652422RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA337230.2733215239545166RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC331380.26858015777969846RNA PCR Primer, Index 1 (100% over 26bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG330480.26785071682972644Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG329930.2674049473602991RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC329210.26682139460032145RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA325380.2637172181132183RNA PCR Primer, Index 1 (100% over 23bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC320260.25956750959782193RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT320170.25949456550282474RNA PCR Primer, Index 1 (97% over 35bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT307260.24903114032044826RNA PCR Primer, Index 1 (100% over 30bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC306770.24863400024768573RNA PCR Primer, Index 1 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT301880.24467070441950442RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC301740.24455723582728653RNA PCR Primer, Index 1 (100% over 26bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC301030.24398178796675307RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC295050.23913505809916116RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG291200.23601467181316974RNA PCR Primer, Index 6 (100% over 34bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA289700.2347989368965497RNA PCR Primer, Index 1 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG286430.23214863477831804No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA282050.22859868882178755RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC278830.22598891120077652RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC275050.222925259210894RNA PCR Primer, Index 1 (100% over 27bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG274370.22237412604869294Illumina Small RNA Adapter 2 (100% over 21bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG269410.2183540959244027RNA PCR Primer, Index 6 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC269110.2181109489410787RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG269080.21808663424274627RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC263270.2133776876657047RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC253310.2053052078193476RNA PCR Primer, Index 1 (100% over 31bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT247510.20060436614175015No Hit
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC241560.19578195097249068RNA PCR Primer, Index 1 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA238860.1935936281225746RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC238350.1931802782509238RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG237270.19230494911095736RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA236970.19206180212763335RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT233430.18919266772441007RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA226560.1836246018062903RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA223730.1813309152636005RNA PCR Primer, Index 1 (100% over 32bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA217030.175900632636031RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC214650.17397166656832722RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC212760.17243984057338596RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC212410.1721561690928413RNA PCR Primer, Index 1 (97% over 36bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG207910.16850896434298118RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT205360.16644221498472714RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG204870.16604507491196457RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG199480.1616765341115766RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT193540.15686222384176124RNA PCR Primer, Index 1 (97% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC192380.15592205550624175RNA PCR Primer, Index 1 (100% over 33bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA191450.15516829985793731RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC191440.15516019495849317RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG188370.15267199082914418RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA187100.1516426685997392RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC185320.15019999649868343RNA PCR Primer, Index 1 (97% over 36bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG185200.15010273770535384RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT181990.14750106498378696RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC177740.1440564827200302RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC175930.14258949592064202RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG175200.14199783826122025RNA PCR Primer, Index 1 (100% over 29bp)
TCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC170770.13840736780746907RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC167200.1355139187059134RNA PCR Primer, Index 1 (100% over 26bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT167140.13546528930924862No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA164460.1332931762582208No Hit
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC157620.12774942503843342RNA PCR Primer, Index 1 (100% over 27bp)
CCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG157510.12766027114454798RNA PCR Primer, Index 6 (100% over 34bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG155360.12591771776405924RNA PCR Primer, Index 6 (100% over 34bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC153160.12413463988634986RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA144510.11712390186717431RNA PCR Primer, Index 1 (100% over 28bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA144280.11693748917995925RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG144280.11693748917995925RNA PCR Primer, Index 1 (100% over 29bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC143340.11617562863221068RNA PCR Primer, Index 18 (100% over 37bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT139950.11342806772064941RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG138250.11205023481514668RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA136210.11039683532854344No Hit
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCA134810.10926214940636474RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC132960.10776274300920002RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT132860.10768169401475869RNA PCR Primer, Index 1 (100% over 30bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA131870.10687930896978946No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC130210.10553389566206331RNA PCR Primer, Index 1 (100% over 27bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG128510.1041560627565606Illumina Small RNA Adapter 2 (100% over 21bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT128350.10402638436545444RNA PCR Primer, Index 1 (100% over 30bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA127580.10340230710825617RNA PCR Primer, Index 1 (100% over 23bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC126240.10231625058274227No Hit

[FAIL]Adapter Content

Adapter graph