FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23629565
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA9623094.072478693534984RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGC9327753.947491204345065RNA PCR Primer, Index 20 (100% over 50bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5600732.370221373097643RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC4889732.0693271331909835RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC4305671.8221537298718788RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA4156401.7589828674374666RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3809321.6120990801142554RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3675121.5553058213301854RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC3242371.3721666056907946RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3177441.344688317368517RNA PCR Primer, Index 1 (100% over 32bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2983411.262575083375424RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2921711.236463726691541RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2576731.0904686565326107RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG2480041.049549579097203RNA PCR Primer, Index 15 (97% over 36bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2366601.0015419242800279RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2330250.9861586533649689RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2028340.8583907490467979RNA PCR Primer, Index 1 (100% over 31bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1967920.8328210866344767RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1868910.7909201883318631RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1846760.781546338241944RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1719370.7276350622620433RNA PCR Primer, Index 6 (100% over 34bp)
ATCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1701190.7199413108112654RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1690600.7154596371113899RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1509390.6387718098069093RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1470090.6221401028753597RNA PCR Primer, Index 1 (100% over 32bp)
ATCTCGGTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1447470.6125673494201015RNA PCR Primer, Index 1 (100% over 32bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1442880.6106248676181725RNA PCR Primer, Index 1 (100% over 32bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1402680.5936122819019309RNA PCR Primer, Index 15 (97% over 36bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1218380.5156167707700078RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1095570.4636437446055397No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1090900.46166740691163805RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1073570.45433337431306925RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1021160.4321535330845066RNA PCR Primer, Index 1 (100% over 26bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC989280.4186619601334176RNA PCR Primer, Index 1 (100% over 33bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA974320.41233090833453767RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG910510.38532660249987677RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC899300.3805825456372134RNA PCR Primer, Index 1 (100% over 26bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG864300.36577059289919217RNA PCR Primer, Index 6 (100% over 34bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC854340.361555534348601RNA PCR Primer, Index 1 (100% over 31bp)
CTCCTGGCTGGCTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA828180.3504846576735543RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC827230.35008261895637943RNA PCR Primer, Index 1 (100% over 27bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG749980.31739052327031836RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC716790.3033445600881777RNA PCR Primer, Index 1 (100% over 27bp)
ATCTCGGTGGGACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA709340.3001917301482274RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG666570.2820915239023655RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC647390.2739745738019299RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA625280.26461765165799706RNA PCR Primer, Index 1 (100% over 28bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC600410.25409270124100886RNA PCR Primer, Index 20 (100% over 39bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC598000.2530727924953337RNA PCR Primer, Index 1 (100% over 27bp)
GTCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA596530.2524506904803368RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC594700.25167623695146313RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA587130.24847262317355395RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC586260.24810444034835175RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA584090.24718609927859445RNA PCR Primer, Index 1 (100% over 32bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT577190.24426602859595595RNA PCR Primer, Index 1 (100% over 25bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA554410.23462556335675247RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC549990.23275502532526518RNA PCR Primer, Index 20 (100% over 39bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT529680.2241598607507163RNA PCR Primer, Index 1 (100% over 30bp)
TCCCCGTACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA509780.2157382076225271RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG485750.20556874407125142RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA455030.19256808155376537RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT452610.19154394082159362RNA PCR Primer, Index 1 (97% over 35bp)
CACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG449180.19009236945326755RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG448780.18992308999340443RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCACTCCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA444960.18830647115171184RNA PCR Primer, Index 1 (100% over 32bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC443670.18776054489365337RNA PCR Primer, Index 1 (100% over 26bp)
TCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC440480.18641054120124512RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC435050.18411257253360358RNA PCR Primer, Index 1 (100% over 26bp)
CCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG434280.1837867095733671RNA PCR Primer, Index 6 (100% over 34bp)
CCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG415810.17597023051418847RNA PCR Primer, Index 6 (100% over 34bp)
CCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG409230.17318558339944048RNA PCR Primer, Index 6 (100% over 34bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT405650.17167053223366577RNA PCR Primer, Index 1 (100% over 30bp)
TCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC403810.1708918467182955RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC399580.16910171643024322RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT399250.16896206087585616RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT396630.16785328041375286RNA PCR Primer, Index 1 (100% over 30bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG386710.163655149809148RNA PCR Primer, Index 6 (100% over 34bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC383820.16243210571163708RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA376530.15934698755563212RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC375390.15886454109502227RNA PCR Primer, Index 1 (100% over 26bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT374270.15839055860740558RNA PCR Primer, Index 1 (97% over 35bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA361640.15304555966222824RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG360810.1526943047830123RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC355890.15061216742669617RNA PCR Primer, Index 1 (100% over 33bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC348320.14740855364878702RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG347850.14720965028344787RNA PCR Primer, Index 6 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC321820.13619378943285668RNA PCR Primer, Index 1 (100% over 31bp)
CCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT321690.13613877360840115RNA PCR Primer, Index 1 (97% over 35bp)
TTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA318250.1346829702535785RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC314210.1329732477089612RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT312740.1323511456939643RNA PCR Primer, Index 1 (97% over 35bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG303500.12844079017112672RNA PCR Primer, Index 15 (97% over 36bp)
ATCCTGTTCGTGACGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA303090.12826727872476704RNA PCR Primer, Index 1 (100% over 32bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC302510.12802182350796554RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC297630.12595661409763573RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC296080.12530065619066624RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG295530.12506789693335446RNA PCR Primer, Index 15 (97% over 36bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC294200.12450504272930966RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC291990.12356977371356602RNA PCR Primer, Index 1 (100% over 27bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC290960.12313387910441854RNA PCR Primer, Index 1 (100% over 31bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG288680.12216898618319888RNA PCR Primer, Index 17 (97% over 37bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA288480.12208434645326732RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC287710.12175848349303088RNA PCR Primer, Index 1 (100% over 27bp)
CATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG285720.12091631818021195RNA PCR Primer, Index 15 (97% over 36bp)
TCCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA274680.11624420508799041RNA PCR Primer, Index 1 (100% over 32bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA273630.11579984650584978RNA PCR Primer, Index 1 (100% over 32bp)
ATCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA262340.11102193375121377RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC257660.10904136407081554RNA PCR Primer, Index 1 (100% over 26bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC252940.10704386644443094RNA PCR Primer, Index 1 (100% over 33bp)
CCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG252310.10677725129514656RNA PCR Primer, Index 6 (100% over 34bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG247570.10477128969576883RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATC247340.10467395400634756RNA PCR Primer, Index 20 (100% over 42bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA246470.10430577118114531RNA PCR Primer, Index 1 (100% over 28bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC242790.10274840015040479RNA PCR Primer, Index 20 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1927800.069.89441
ATTCAAC865250.069.8477864
TCAACGC860000.069.846956
CAACGCT861350.069.838637
TAGCTTA1147200.069.820191
TCAGTGC182300.069.80121
GAGGTAG1993400.069.7984542
ACGTAAA489550.069.79318
TATTGCA794200.069.7920461
GCAGCAC506200.069.781423
CGCTGTC866600.069.7785910
TATAGTT1646850.069.7695716
TAGTAGA1290500.069.768356
TTGTATA1665400.069.7591913
ATAGTTT1396800.069.755917
AAGCTGC333000.069.7496261
ACGCTGT864850.069.746069
TGTATAG1664900.069.7397514
AACATTC940200.069.7331851
GGTAGTA1993550.069.730434