FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006135081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14901857
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGC7649835.133474304578281RNA PCR Primer, Index 27 (100% over 50bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT5763233.867457592701366RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT3765962.527174968864619RNA PCR Primer, Index 8 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3034712.0364643144810746RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2954361.9825448600130844RNA PCR Primer, Index 1 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2773451.861143882940227RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2686531.8028155819774676RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2340291.5704687006458322RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2090921.403127140463098RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2002111.3435305411936245RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1850081.2415096990932069RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1597691.0721415458489503RNA PCR Primer, Index 1 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1567511.0518890363798283RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1554491.043151870266907RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1445910.9702884680748178RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1418840.952122946824681RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1069180.7174810495094672RNA PCR Primer, Index 1 (100% over 32bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1044500.7009193552186147RNA PCR Primer, Index 1 (100% over 23bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT870930.5844439387654841RNA PCR Primer, Index 27 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC866210.5812765482852238RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA861310.5779883674900383RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC831860.5582257298536686RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG821250.5511058118461343RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA637620.42787955890329643RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC631970.42408808512925605RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA630820.4233163692283452RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC614240.4121902391091258RNA PCR Primer, Index 1 (100% over 31bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG608200.4081370529860809Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA573720.38499899710485747RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA560710.3762685415649875RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG545710.36620268198788913RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA540820.3629212117657551RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC483450.32442265416987964RNA PCR Primer, Index 13 (97% over 37bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG474150.31818182123207867No Hit
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC436180.292701775355917RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA434970.2918897960166978RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA391350.2626182763664958RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG382580.25673310380041897RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA378190.25378716223085485RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC374400.2512438550443747RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC366860.2461840829636199RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC358780.24076193993808961RNA PCR Primer, Index 1 (100% over 27bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT358280.24042641128551967RNA PCR Primer, Index 27 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG355530.23858100369638494RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC349890.23479624049539596RNA PCR Primer, Index 1 (100% over 26bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG345200.23164898173428988Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA341150.22893119964847333RNA PCR Primer, Index 1 (100% over 28bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA328910.2207174582335611RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA320290.21493294426325524RNA PCR Primer, Index 1 (100% over 32bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG308770.2072023641080437Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA305620.20508853359685306RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCC303230.20348470663756874RNA PCR Primer, Index 27 (100% over 38bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG295980.19861954117530453RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC293510.19696202963160903RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG292530.1963043934725719RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA285320.1914660703025133RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC281410.18884223623941634RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT278010.18656064140194073RNA PCR Primer, Index 1 (100% over 30bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA276550.1855808977364365RNA PCR Primer, Index 1 (100% over 22bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA267860.17974940975477083RNA PCR Primer, Index 1 (100% over 34bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT266030.17852137488636483RNA PCR Primer, Index 8 (97% over 36bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA264820.17770939554714557RNA PCR Primer, Index 1 (100% over 32bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT263660.1769309690731833RNA PCR Primer, Index 1 (100% over 30bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATC259800.17434068787534332RNA PCR Primer, Index 27 (100% over 42bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT255970.17177053839665754RNA PCR Primer, Index 8 (97% over 36bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG253790.17030763347145259RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA248440.16671747688895416RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA248220.16656984428182342RNA PCR Primer, Index 1 (100% over 28bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG245290.16460364637776354Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC244240.16389903620736662RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC240600.16145638761665745RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC237950.15967808575803674RNA PCR Primer, Index 1 (100% over 27bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA224860.15089394563375558RNA PCR Primer, Index 1 (100% over 22bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTAT224000.15031683635133528RNA PCR Primer, Index 27 (100% over 41bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTAT220980.1482902432898128RNA PCR Primer, Index 27 (100% over 41bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC219030.14698168154479002RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC214690.14406929284048292RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG212870.1428479685451283RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG212480.14258625619612375RNA PCR Primer, Index 1 (100% over 29bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA212020.1422775698357594RNA PCR Primer, Index 1 (100% over 23bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA210510.14126427330499816RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC206410.13851293835392461RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG204060.13693595368684586RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT193780.1300374845900078RNA PCR Primer, Index 1 (100% over 35bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA192880.1294335330153819No Hit
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA192660.12928590040825114RNA PCR Primer, Index 1 (100% over 23bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG179610.12052860257617556Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC177530.1191328033814846RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT175960.11807924341241498RNA PCR Primer, Index 1 (100% over 30bp)
CTGCTGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC173650.11652910103754184RNA PCR Primer, Index 13 (97% over 37bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT173360.11633449441905126RNA PCR Primer, Index 1 (100% over 30bp)
AGGTCGCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT172330.11564330539475717RNA PCR Primer, Index 27 (100% over 39bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG169360.1136502651984917Illumina Small RNA Adapter 2 (100% over 21bp)
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT166850.11196591136259058RNA PCR Primer, Index 1 (100% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA165590.11112037915811432RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC165090.11078485050554437RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC162400.10897970635471807RNA PCR Primer, Index 1 (100% over 26bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG162050.10874483629791912Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC161040.10806706841972782RNA PCR Primer, Index 1 (100% over 31bp)
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTCGGGTGCCAAGGAA160440.10766443403664389RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC160170.10748324856425613RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA159480.10702021902370959RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT158040.10605389650430815RNA PCR Primer, Index 1 (100% over 30bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC157680.1058123158744578RNA PCR Primer, Index 13 (97% over 37bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA153920.1032891404071318RNA PCR Primer, Index 1 (100% over 34bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT149670.10043714686028729RNA PCR Primer, Index 1 (100% over 30bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTA149090.10004793362330615RNA PCR Primer, Index 27 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph