FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194351
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3114515.027984368338184RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC2624084.2362468642800515RNA PCR Primer, Index 1 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1671562.6985232189780657RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC1623632.6211462669777674RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1523812.4599994414265516RNA PCR Primer, Index 6 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1236021.9953987108576832RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1197361.932987007032698RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC1119981.8080667369349914RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC948191.530733405323657RNA PCR Primer, Index 18 (100% over 50bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA928711.499285397291823RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA926021.4949427308849628RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA912911.4737782860545037RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA864701.3959493092981008RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA791751.278180716591617RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA776651.2538036672445587RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC731751.1813182688549615RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA690901.1153710856875885RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC689691.113417692991566RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC685151.1060884344461592RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC596780.963426192671355RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA518310.8367462547730989RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA491500.7934648843761034RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC465340.7512328571629214RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA400610.6467344197963596RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG382400.6173366669082847RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351490.5674363625826176No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC347520.561027297290709RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC296670.4789363728338933RNA PCR Primer, Index 1 (97% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC277640.44821483316008404RNA PCR Primer, Index 18 (100% over 37bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC263810.42588803895678495RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA262860.42435438353428795RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC257530.415749769427015RNA PCR Primer, Index 1 (100% over 33bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC256360.4138609516961503RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC245560.39642571110355224RNA PCR Primer, Index 18 (100% over 39bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC240500.3882569780110943RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC239710.38698162244922835RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC211720.3417952905800785RNA PCR Primer, Index 1 (100% over 26bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC206410.33322296395538453RNA PCR Primer, Index 18 (100% over 39bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG198190.3199528086154627RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC191040.30841003359351127RNA PCR Primer, Index 18 (100% over 42bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA190210.3070701030664875RNA PCR Primer, Index 1 (100% over 32bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC179510.28979629988678396RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA175840.28387154683355853RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG174090.28104639210790605RNA PCR Primer, Index 6 (100% over 34bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA173810.28059436735180165RNA PCR Primer, Index 1 (100% over 32bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCAT170210.2747826204876023RNA PCR Primer, Index 18 (100% over 41bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA167640.2706336789762156RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC162700.26265867077923094RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG158020.2551033998557718RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC149120.24073547010816793RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG147200.23763587178059495RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA139860.2257863656741441RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT137450.22189572402338842RNA PCR Primer, Index 1 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC137150.22141141178470514RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC131470.21224176673230172RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG127820.2063493011616552RNA PCR Primer, Index 1 (100% over 29bp)
AGGTCGCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC125670.202878396784425RNA PCR Primer, Index 18 (100% over 39bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC121240.19572671939320196RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC118950.1920298026379196RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC118110.19067372836960644RNA PCR Primer, Index 1 (100% over 26bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA116870.18867190444971554RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC116830.1886073294845578RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA110240.17796860397481512RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA109640.17699997949744858RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA108980.17593449257234536RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG108960.17590220508976645RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT105360.17009045822556712RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC104720.16905725878304279RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA104260.16831464668372845RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC104120.16808863430567625RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG103540.16715229731088857RNA PCR Primer, Index 18 (100% over 38bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC102060.16476302360005107RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC99850.16119525677508428RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC99080.1599521886957972RNA PCR Primer, Index 1 (100% over 33bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA96810.15628755942309372RNA PCR Primer, Index 1 (100% over 32bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC96190.15528664746314827RNA PCR Primer, Index 1 (100% over 33bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT93140.1503628063698683RNA PCR Primer, Index 1 (97% over 35bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT90600.14626229608234986RNA PCR Primer, Index 1 (100% over 30bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC90260.1457134088785088RNA PCR Primer, Index 1 (100% over 33bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG88990.1436631537347496RNA PCR Primer, Index 6 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG85760.13844872529825963RNA PCR Primer, Index 6 (100% over 34bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA85690.13833571910923356RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC84640.13664062627384208RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC81790.13203966000635095RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT80010.12916607405683017RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC79490.12832659950977915RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA78990.127519412445307RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA78260.12634091933117772RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCC77630.12532386362994283RNA PCR Primer, Index 1 (100% over 27bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG77360.12488798261512787RNA PCR Primer, Index 6 (100% over 34bp)
ATCCCACTCCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA75500.12188524673529155RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC75500.12188524673529155RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC75050.12115877837726664RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA73790.11912466697479689RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC73190.11815604249743032RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC72390.11686454319427492RNA PCR Primer, Index 1 (100% over 27bp)
CAACACCAGTCGATGGGCTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA72130.11644480592074942RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC69810.11269945794159873RNA PCR Primer, Index 1 (100% over 33bp)
ATCTCGGTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA65600.1059029428587434RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC65570.10585451163487507RNA PCR Primer, Index 1 (100% over 33bp)
GGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT64750.10453072484914076RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC64600.10428856872979914RNA PCR Primer, Index 1 (100% over 26bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG63830.10304550065051207RNA PCR Primer, Index 6 (100% over 34bp)
TCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC62940.10160870767575167RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph