FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10840557
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA6133205.657642868350768RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC4238173.9095500351135093RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3983323.6744606388767664RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3119562.8776750124555406RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2550122.352388350524793RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC2188962.019232037615779RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC2119951.9555729470358396RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1904691.757003814471895RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1752971.6170479063022314RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1742861.6077218172461063RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGC1694071.5627149047784168RNA PCR Primer, Index 19 (100% over 50bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC1445071.333021910221034RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1411161.3017412297172553RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1381091.2740028026235184RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1222611.127811052513261RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA976920.9011714065983879RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC914960.8440156718884463RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA849770.7838803854820374RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA849410.7835482992248461RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA786800.725792964328309RNA PCR Primer, Index 1 (100% over 32bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA786200.72523948723299RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA762520.7033955912044002RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA665580.6139721418373614RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC661980.6106512792654473RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC623220.5748966589078403RNA PCR Primer, Index 1 (100% over 33bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG585740.5403227896869137RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG546020.5036826059767961RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA529650.48858190589284295RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC499820.4610648696372336RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC488000.45016137085944935RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC482880.44543836631272726RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG461150.4253932708439243RNA PCR Primer, Index 15 (97% over 36bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA455750.42041197698605337RNA PCR Primer, Index 1 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC421290.38862394247823245RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT405300.3738737778879812RNA PCR Primer, Index 1 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC351160.32393169465369726RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC346050.3192179147252304RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC342980.31638595692084825RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGG335610.30958741326667993RNA PCR Primer, Index 19 (97% over 49bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA332960.307142889429021RNA PCR Primer, Index 1 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC330750.3051042487945961RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC321060.2961655937051943RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA316860.2922912540379613RNA PCR Primer, Index 1 (100% over 32bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC297630.27455231313298756RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG292790.2700875978974143RNA PCR Primer, Index 15 (97% over 36bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA292680.2699861270966058RNA PCR Primer, Index 1 (100% over 32bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA291110.2685378620305211RNA PCR Primer, Index 19 (100% over 39bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA289920.26744013245813847RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG283480.26149947830171455RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC282320.26042942258409785RNA PCR Primer, Index 1 (100% over 26bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG281040.2592486714474173RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC270890.24988568391827096RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC238410.2199241238250027RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG233870.21573614713708897RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA233490.21538561164338696RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228680.21094857026257968No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC221800.20460203290292187RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC220600.2034950787122839RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC219030.2020468136461992RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC214940.1982739447797747RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC212050.1956080301039882RNA PCR Primer, Index 1 (100% over 26bp)
ATCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA211900.19546966083015846RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC210980.1946209959506693RNA PCR Primer, Index 1 (100% over 27bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT210280.19397527267279718RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC207170.19110641639539372RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG205170.1892614927443304RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCC197560.18224155825203447RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT195200.18006454834377977RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC193980.17893914491663113RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG186250.1718085150052714RNA PCR Primer, Index 6 (100% over 34bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT182020.1679065014832725RNA PCR Primer, Index 1 (100% over 25bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA175630.16201197041812518RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA170130.15693843037770108RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC168060.15502893439885054RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG167780.15477064508770166RNA PCR Primer, Index 1 (100% over 29bp)
AGGTCGCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA158600.14630244552932106RNA PCR Primer, Index 19 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA155850.143765675509109RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT155850.143765675509109RNA PCR Primer, Index 1 (100% over 25bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG155730.14365498009004518RNA PCR Primer, Index 1 (100% over 29bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG152510.14068465301183325RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC152440.14062008068404602RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA149520.1379264921534936RNA PCR Primer, Index 1 (100% over 32bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG145870.13455950649030304RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT143380.13226257654472923RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC142330.13129399162792096RNA PCR Primer, Index 1 (100% over 33bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT139770.12893248935455992No Hit
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC138230.12751189814324118RNA PCR Primer, Index 1 (100% over 27bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG137810.12712446417651788Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA135840.1253072143802205RNA PCR Primer, Index 1 (100% over 22bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC133030.12271509665047653RNA PCR Primer, Index 1 (100% over 33bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC132820.12252137966711489RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC131370.12118381002009398RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA130870.12072257910732817RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC130550.12042739132315802RNA PCR Primer, Index 1 (100% over 33bp)
ACTGGACTTGGAGTCAGAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC129510.11946803102460508RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC128440.11848099687128623RNA PCR Primer, Index 1 (100% over 27bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAA128260.11831495374269053RNA PCR Primer, Index 19 (100% over 40bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA127050.11719877493379723RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT124490.11483727266043617RNA PCR Primer, Index 1 (97% over 35bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC121090.11170090245362853RNA PCR Primer, Index 1 (100% over 26bp)
ATCTCGGTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA120740.11137804081469246RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCGCGTATGC120100.11078766524635218RNA PCR Primer, Index 19 (98% over 50bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA118930.10970838491048014RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG117290.10819554751660823RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG116860.10779888893162962RNA PCR Primer, Index 15 (97% over 36bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT116800.10774354122209773RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC115640.10667348550448098RNA PCR Primer, Index 1 (100% over 26bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA115160.1062307038282258RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGA207.914431E-570.0306851
GCACCTA207.9234786E-570.0145346
CAACGGA500800.069.946781
ACATCCC61550.069.900776
TATTGCA891500.069.893211
TAGCAGC472400.069.8898541
GCACGTA460100.069.885186
TGAGGTA1236450.069.8777541
CAGCACG460100.069.87664
TTCAACG426050.069.86635
TCAACGC426300.069.825656
AGCACGT460450.069.8241355
GCAGCAC474850.069.81293
ATTCAAC428700.069.809414
CAACGCT426500.069.804977
TAGCTTA529000.069.792391
GGTAGTA1276100.069.791354
ACGCTGT427050.069.787879
GTAGTAG1264250.069.787145
TCAGTGC83250.069.778321