FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809923
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA4450575.051769464954461RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3170363.598623960731552RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA2476192.8106829083523204No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2461522.7940312304659187RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2134062.4223367218987044RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2092272.3749015740546198RNA PCR Primer, Index 1 (100% over 29bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1839172.0876118894569227RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1539701.74768837366683RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1414831.605950471984829RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1333201.5132935895126438RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1276091.44846895937683RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC967851.0985907595333126RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG899461.0209623852558076RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC878790.9975002051663788RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT808350.9175449093028396RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC764960.8682936275379478RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA751790.8533445752023031No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG627960.7127871605688267RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA622980.7071344437403142RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA619130.7027643714933718RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA613130.6959538692903445RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC608030.6901649424177714RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC605790.6876223549286412RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA591200.6710614837382801RNA PCR Primer, Index 3 (97% over 36bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA557640.632968074749348RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC542830.6161574851448758RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA534640.6068611496377437RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT532930.604920156509881RNA PCR Primer, Index 1 (97% over 35bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT520610.5909359253196651RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA507800.576395503116202RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC490150.556361275802297RNA PCR Primer, Index 17 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA483110.5483702865507452RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC467360.5304927182677988RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG464570.5273258347433911RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA428030.4858498763269554RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA421220.47811995632651955RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA394300.44756350310893755RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC367920.41761999508962794RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA361890.4107754403755856RNA PCR Primer, Index 1 (100% over 32bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA355320.4033179404632708RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT339670.3855538805503748RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC333160.3781644856600903RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT307070.348550151913927RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG298270.33856141534948714RNA PCR Primer, Index 1 (100% over 29bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA293960.33366920460031263RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA279260.31698347420289597RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC278540.3161662139385327RNA PCR Primer, Index 1 (100% over 33bp)
TGTCAGTTTGTCAAATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA276140.31344201305732183RNA PCR Primer, Index 1 (100% over 32bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG275520.31273826116300907RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA267370.3034873290038971RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC255620.29015009552296883RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC252090.2861432500601878RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG249820.2835666100600425RNA PCR Primer, Index 1 (100% over 29bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA246780.2801159556105087RNA PCR Primer, Index 1 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA242290.2750194297952434RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC220470.25025190345023446RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG206590.23449694168723154RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG203450.2309327788676473RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA199820.22681242503481586RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175620.19934339948260615No Hit
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC175130.19878720846935893RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA169830.1927712648566849RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC163280.1853364666183802RNA PCR Primer, Index 1 (100% over 27bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA161490.1833046667944771RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC155930.17699360141967188RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC155320.1763012003623641RNA PCR Primer, Index 1 (100% over 27bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG154190.175018555780794Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA153190.17388347208028948RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCA152660.17328187771902206RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC150700.17105711366603318RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC148840.16894585798309475RNA PCR Primer, Index 1 (100% over 27bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA147770.16773131842355488RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT142130.16132944635270932RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC140360.15932034820281632No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC138540.15725449586789805RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA133360.15137476229928457RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA131370.14911594573528056No Hit
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA123080.13970610185809795RNA PCR Primer, Index 1 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA116990.13279344212202535RNA PCR Primer, Index 1 (100% over 23bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG116850.13263453040395473RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG116560.1323053561308084RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG116550.13229400529380336RNA PCR Primer, Index 1 (100% over 29bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC113270.12857093075614848RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC112520.1277196179807701RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG111090.12609644828904862RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG107440.12195339278220706RNA PCR Primer, Index 1 (100% over 29bp)
AGTGTTTCCTACTTTATGGATGTGGAATTCTCGGGTGCCAAGGAACTCCA105950.12026211806845531RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT105660.11993294379530899RNA PCR Primer, Index 1 (100% over 30bp)
TTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAAC105160.11936540194505672RNA PCR Primer, Index 1 (100% over 24bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC104500.11861624670272373RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGTGCGGACCAGGGGAATCCGATGGAATTCTCGGGTGCCAAGGAACT104020.11807140652648154RNA PCR Primer, Index 1 (100% over 25bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTC103990.11803735401546642RNA PCR Primer, Index 1 (100% over 26bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT103920.11795789815643111RNA PCR Primer, Index 1 (100% over 30bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC103240.11718604124008802RNA PCR Primer, Index 1 (100% over 33bp)
TCAGTGCACTACAGAACTTTGTATGGAATTCTCGGGTGCCAAGGAACTCC101280.11496127718709913RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG100950.11458669956593265RNA PCR Primer, Index 3 (97% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA100200.11373538679055424No Hit
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC97620.11080687084325255RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC97060.11017122397097001RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG97000.11010311894893975RNA PCR Primer, Index 1 (100% over 29bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA95800.1087410185083343RNA PCR Primer, Index 1 (100% over 28bp)
TAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA92340.1048136289045886RNA PCR Primer, Index 1 (100% over 32bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG91160.10347423013799327Illumina Small RNA Adapter 2 (100% over 21bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA90720.10297479330977127RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTTGGAATTCTCGGGTGCCAAGGAACT90250.10244130397053412RNA PCR Primer, Index 1 (100% over 25bp)
CATAAAGTAGAAAGCACTACTATGGAATTCTCGGGTGCCAAGGAACTCCA89630.10173755207622133RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTGC396350.069.9047241
TAGCAGC269300.069.88121
CCCATAA207700.069.868651
AGTGCAC390050.069.8400963
TATTGCA464700.069.811171
GGTCCGA408600.069.81085
TTCAAGT654050.069.807021
GTGCACT390400.069.801614
GTCCGAT366500.069.797316
TGGTCCG408900.069.7766954
CAGCACG254100.069.773644
TTCAACG189250.069.767385
TCCTACA57700.069.75299
TCAACGC189500.069.7488256
TAATCCA564350.069.741267
GCAGCAC271600.069.739953
CAACGCT189500.069.736957
AAGTAAT664200.069.7285164
TGCACTA391000.069.721355
AACATTC199200.069.720811