FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9145623
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA3783444.136886027337886RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2496052.729229053067243RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1963982.147453486766292RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1707621.8671445346041489RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1542991.6871349278228505RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1441841.5765355733556914RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1176311.2861999669131343RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1161961.2705094010544715RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1143181.2499749880352602RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA958971.04855623285587RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG956971.0463693944086696RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG955741.0450244887636413No Hit
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC950821.0396448661835285RNA PCR Primer, Index 1 (100% over 27bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT884090.966681001392688No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC863810.9445064595380763RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT852570.9322164274648101No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG756690.8273793923060244Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG754010.824449028786776RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA604830.6613327490101003RNA PCR Primer, Index 1 (100% over 32bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG594580.6501252019681983Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA585090.6397486535362326RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA558950.6111666750313237RNA PCR Primer, Index 1 (100% over 28bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC544550.5954214382114811No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC537680.5879096481453479RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA532520.5822676049515708RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC532500.5822457365670988RNA PCR Primer, Index 1 (100% over 26bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG522720.571552096560289No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA482520.5275966437715616No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC480350.5252239240563492RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG449110.49106550751107936Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT436620.47740870140831304No Hit
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG409720.44799572429346807No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA392320.42897022980282484RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA376410.41157392995534586RNA PCR Primer, Index 1 (100% over 28bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA372660.40747360786684517RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA369710.4042480211572246No Hit
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC357710.3911269904740224RNA PCR Primer, Index 1 (100% over 27bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG351710.38456647513242126Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG349000.3816033090364648RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG337710.36925860600201865RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC317670.34734648476107094RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA307790.3365435028319011RNA PCR Primer, Index 1 (100% over 22bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC306940.33561409649184093No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA301400.3295565539930959No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA300750.3288458314977558RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG300570.3286490160375078RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA284980.3116026103415809RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG279640.30576375168755593Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC278090.3040689518909756RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC276200.3020023895583712RNA PCR Primer, Index 1 (100% over 31bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG276160.30195865278942724No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA264780.28951554202485713RNA PCR Primer, Index 1 (100% over 23bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG263830.28847679376243696No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA260140.2844420768273523No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA256430.28038549150779557RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC238400.26067114290628424RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC238260.2605180642149802RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG234900.2568441756236836RNA PCR Primer, Index 2 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA229530.2509725143929506RNA PCR Primer, Index 1 (100% over 34bp)
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGGGTGCCAA227850.24913557009730228No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA227450.2486982024078622RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC226600.24776879606780203No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA224080.2450133796243296RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC223050.2438871578240214RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT222220.24297961986843325RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG221510.2422032922196771RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG216410.23662685417931614RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT213270.23319351781721157No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG208960.22848088096349478No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC199690.21834488476072106RNA PCR Primer, Index 1 (100% over 27bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA199620.21826834541506906RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG198410.21694530815451282RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC193610.21169689588123194RNA PCR Primer, Index 1 (100% over 26bp)
TAATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCG191300.20917109747471552No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCG188300.20589083980391493No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC186390.2038024090868386RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT185090.20238096409615833RNA PCR Primer, Index 8 (97% over 36bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC183420.20055495399274606No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC176100.1925511252759927RNA PCR Primer, Index 1 (100% over 33bp)
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC168980.18476598040395936No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA162410.17758221610490615RNA PCR Primer, Index 1 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC162370.17753847933596215RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG160140.1751001544673337RNA PCR Primer, Index 1 (100% over 29bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT154320.16873645458598063No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA151900.1660903800648682RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG142990.15634801478259053No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT138940.15191966692700978RNA PCR Primer, Index 8 (97% over 36bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG138840.15181032500464978Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA138810.15177752242794176RNA PCR Primer, Index 1 (100% over 32bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA137690.15055289289750956RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC134780.147371042956833No Hit
TGGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAG134480.14704301718975296No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA134260.14680246496056093RNA PCR Primer, Index 1 (100% over 22bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC129700.14181647330094407No Hit
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC129320.141400973995976RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT126030.1378036247503314No Hit
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA125350.13706009967828325RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC124640.13628377202952713RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC123790.135354365689467RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC123760.13532156311275897RNA PCR Primer, Index 1 (100% over 33bp)
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCTCGGGTGCCAAGGA122310.1337361052385387RNA PCR Primer, Index 1 (100% over 22bp)
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG121960.13335340851027863No Hit
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA121300.1326317518227025RNA PCR Primer, Index 1 (100% over 23bp)
GTTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGA120040.13125404360096626RNA PCR Primer, Index 1 (100% over 22bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC119890.13109003071742625No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC119850.13104629394848225RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC119770.13095882041059423RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC117330.12829087750500978RNA PCR Primer, Index 1 (100% over 33bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGA116430.1273068002037696No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC116070.12691316928327354RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCC114750.1254698559081213RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT114610.12531677721681728No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCTGGAATTCTCGGGT113640.12425616056992508No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC113430.12402654253296905RNA PCR Primer, Index 1 (100% over 27bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGAA112920.12346889872893296RNA PCR Primer, Index 1 (100% over 23bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT111860.12230987435191676No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC110090.12037452232614443No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA101630.11112419569448687RNA PCR Primer, Index 13 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC99080.10833597667430639RNA PCR Primer, Index 13 (100% over 50bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT98630.1078439380236863RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA96400.10540561315505789RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT95660.10459648292959377RNA PCR Primer, Index 1 (100% over 25bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG94490.10331718243798153Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG94440.10326251147680153RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA94360.1031750379389135RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC92820.10149117233456922RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA92320.10094446272276913RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT92190.10080231822370112RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTA207.928675E-570.0051421
GCTCGAT207.92932E-570.00418
CGGTCAT207.930822E-570.0013215
GCGAACA150.002225716869.9974910
AGTTACC455.456968E-1269.9974911
TTACGTT150.00222576569.997116
GCCACTA600.069.997117
CGCTGTC395950.069.7234810
TTAATGC11350.069.718481
CCCATAA158150.069.717021
TCAACGC395550.069.713976
CAACGCT395500.069.7139367
TTCAACG396300.069.670355
TGAGGTA586650.069.656931
ACGCTGT396600.069.644129
ATTCAAC400400.069.638734
TTCAGGA37950.069.6285910
AACATTC421350.069.619791
TAATCCA342100.069.608357
GGATAGG371150.069.6037214
Produced by FastQC (version 0.11.5)