FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8560686
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA4453985.202830707725993RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA4054144.735765334693972RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3638994.250815880876837RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3017063.524320364045592RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2277342.660230733845395RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2141932.5020541578093156RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC1696591.981838838616438RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC1542301.8016079552503153RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1149451.3427078157054235RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1141441.333351088919743RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1103361.288868672440503RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1084801.2671881669296128RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGC977511.141859425751628RNA PCR Primer, Index 27 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA912111.0654636789621765RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA862631.007664572675601RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA837170.9779239654392183RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA773200.9031986455291083RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA700530.8183105886607687RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG657450.7679875187572585RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC614520.7178396684564765RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA576520.6734507024320247RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA563700.6584752670521965RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC554650.6479036843542679RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA524630.612836401194951RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT496020.5794161823012782RNA PCR Primer, Index 8 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT420740.49147930434546955RNA PCR Primer, Index 1 (100% over 35bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC406630.47499698038217963RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA404540.4725555872508348RNA PCR Primer, Index 1 (100% over 32bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC380930.44497602178143203RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG367370.4291361696948119RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC352930.4122683626055202RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC335970.39245686619039644RNA PCR Primer, Index 1 (100% over 27bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA332850.38881229845365195RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC323400.3777734634817817RNA PCR Primer, Index 1 (100% over 31bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA323090.377411342969477RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312110.3645852680497801No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA309750.3618284796335247RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC294520.34403784930319836RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC292350.34150300571706516RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA290320.33913170042681157RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT263080.3073118205713888RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC258950.30248744084294177RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA257000.3002095860074765RNA PCR Primer, Index 1 (100% over 34bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC252000.29436893258320657RNA PCR Primer, Index 1 (100% over 33bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA248720.29053746393688545RNA PCR Primer, Index 1 (100% over 22bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA243580.2845332722167359RNA PCR Primer, Index 1 (100% over 32bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC241710.28234886783605895RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA239800.2801177382279878RNA PCR Primer, Index 1 (100% over 28bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT229780.2684130687657508RNA PCR Primer, Index 27 (100% over 39bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT215720.25198915133670363RNA PCR Primer, Index 27 (100% over 39bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT206690.24144093125247207RNA PCR Primer, Index 8 (97% over 36bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC195640.22853308718483542RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA194700.22743504434107267RNA PCR Primer, Index 1 (100% over 34bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC193100.22556603524530627RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA192160.22446799240154353RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG191460.22365030092214572RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC185510.21669992334726446RNA PCR Primer, Index 1 (100% over 26bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG183810.21471410118301268RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT177930.20784549275607117RNA PCR Primer, Index 1 (100% over 35bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC176410.20606993411509314RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG166020.19393305629946012RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA165120.19288173868309152RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA156590.18291758394128693RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG155230.18132892620988553RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT151520.1769951613690772RNA PCR Primer, Index 1 (100% over 35bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG149800.17498597659112833RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC149580.17472898784046045RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA145810.1703251351585609RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC143040.16708941316151532RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT139800.1633046697425884RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC139260.16267387917276724RNA PCR Primer, Index 1 (100% over 33bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA137540.16066469439481834RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG128220.14977771641197915RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC126000.14718446629160328RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG125870.14703260930257225RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC124560.1455023581054135RNA PCR Primer, Index 1 (100% over 27bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA124490.14542058895747373RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC122660.14328290980419092RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC119350.13941639723732419RNA PCR Primer, Index 1 (100% over 26bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC117850.13766420121004322RNA PCR Primer, Index 13 (97% over 37bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT117200.1369049162648881RNA PCR Primer, Index 1 (100% over 30bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA110390.12894994630103243RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC109970.12845933141339375RNA PCR Primer, Index 1 (100% over 27bp)
AGGTCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT109180.1275365081723591RNA PCR Primer, Index 27 (100% over 39bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA108910.1272211128874485RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC108820.12711598112581166RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTCCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA108180.1263683774875051RNA PCR Primer, Index 1 (100% over 32bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA106230.12409052265203979RNA PCR Primer, Index 1 (100% over 28bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT104650.1222448761699705No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCC100930.11789943002231364RNA PCR Primer, Index 27 (100% over 38bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC100540.11744385905522058RNA PCR Primer, Index 1 (100% over 27bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG99340.11604210223339578Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA97510.11390442308011298RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT91320.10667369414086676RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC90420.10562237652449816RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG89970.10509671771631386Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA89500.10454769629443247RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG89390.10441920191909854RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC89350.10437247669170438RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC89130.10411548794103649RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA87320.10200117140145078RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG500.070.052221
GAACGTA850.070.0190666
TAATGGG150.002222993470.0190666
TTTTATC150.002223405570.01582
GGTAATA207.922677E-570.015794
TTGCTAC150.002224693570.005577
AACCTAC252.8412178E-670.005577
CGATCCA150.002225260570.00107611
TATTATC150.0022255769.9986213
CGATAAT800.069.9969819
GTATTAG455.456968E-1269.99657421
CAACGGA443400.069.957421
AAGCTGC248000.069.953351
TATTGCA474150.069.934021
TTCAACG372850.069.915785
TTCAAGT632600.069.902721
CAACGCT372800.069.902297
ATTCAAC376050.069.885464
TCAACGC372850.069.878226
TAGCTTA500950.069.870421
Produced by FastQC (version 0.11.5)