FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20088002
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC500239524.902401941218443RNA PCR Primer, Index 26 (100% over 50bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5545242.7604736399369134RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT4966972.4726052894658213RNA PCR Primer, Index 1 (100% over 35bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG4232722.1070885994535447RNA PCR Primer, Index 1 (100% over 29bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA3936631.9596921585332379RNA PCR Primer, Index 1 (100% over 34bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3921831.952324576630369RNA PCR Primer, Index 1 (100% over 33bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3780901.8821682713890608RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2953751.470405070648639RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC2522961.255953678220462RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2399861.1946733179337596RNA PCR Primer, Index 1 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2316721.153285428784804RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1860390.9261199794782975RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1766380.8793209001074374RNA PCR Primer, Index 1 (100% over 26bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1756500.874402541377684RNA PCR Primer, Index 15 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGG1716980.8547291064586712RNA PCR Primer, Index 26 (97% over 49bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1703110.8478244874726716RNA PCR Primer, Index 1 (100% over 35bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA1542660.767950939072985RNA PCR Primer, Index 1 (100% over 28bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC1499730.746579973458784RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1311320.6527876689777311RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTGGTATGC1296680.645499736608947RNA PCR Primer, Index 26 (98% over 50bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1235620.6151034831637312RNA PCR Primer, Index 1 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1195250.5950069100948915No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1180440.587634350096142RNA PCR Primer, Index 15 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGCGTATGC1100180.5476801525607176RNA PCR Primer, Index 26 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGGATGC1061620.5284846148462151RNA PCR Primer, Index 26 (98% over 50bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA982730.4892124164463942RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTGGTATGG860510.4283701285971596RNA PCR Primer, Index 26 (97% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTAGTATGC857260.42675224743605655RNA PCR Primer, Index 26 (98% over 50bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA768430.38253182173120054RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG760050.3783601773834949No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA757630.37715547818045814RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC690100.3435383967006773RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA584800.29111904708093916RNA PCR Primer, Index 1 (97% over 37bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC574380.2859318711736488RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA560190.278867953119479RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGGATCTCGTATGC534660.2661588743370296RNA PCR Primer, Index 26 (98% over 50bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA494820.24632614034984665RNA PCR Primer, Index 1 (100% over 32bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC492020.24493227350335786RNA PCR Primer, Index 26 (100% over 39bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG491380.24461367536701759RNA PCR Primer, Index 26 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTGGGATGG470990.2344633378670512RNA PCR Primer, Index 26 (97% over 45bp)
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC469890.23391574732021633RNA PCR Primer, Index 26 (98% over 50bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC452750.22538329098135296RNA PCR Primer, Index 1 (100% over 33bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA442250.22015629030702008RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG431580.21484466200272184RNA PCR Primer, Index 1 (100% over 29bp)
TCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTC424990.2115640968175929RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA415190.2066855628548822RNA PCR Primer, Index 1 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA404970.20159794886519825RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGGATGG389010.1936529078402123RNA PCR Primer, Index 26 (97% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATATCGTATGC387990.19314514206041994RNA PCR Primer, Index 26 (98% over 50bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA383120.19072080936670555RNA PCR Primer, Index 1 (100% over 28bp)
CAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA382440.190382298846844RNA PCR Primer, Index 1 (97% over 37bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC375200.18677815742949447RNA PCR Primer, Index 1 (100% over 26bp)
TTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAAC357890.17816107346066573RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC352760.1756073102740631RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA327170.16286836291633186RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG313770.15619771443670705RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA303970.15131918047399637No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC296390.14754578379671607RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGGGCATCTCGTATGC284490.14162184969913882RNA PCR Primer, Index 26 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCAAGTCACATGAGCATCTCGTATGC283600.1411787991657906RNA PCR Primer, Index 26 (98% over 50bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT281420.1400935742638815RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAGCTCGTATGC276330.13755972346080014RNA PCR Primer, Index 26 (98% over 50bp)
GTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC275290.13704200148924717RNA PCR Primer, Index 26 (100% over 39bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC273580.13619074709371295RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGCGCATCTCGTATGC272650.13572778417684347RNA PCR Primer, Index 26 (98% over 50bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT270560.13468736213785723RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTGGGATGC270240.13452806306968706RNA PCR Primer, Index 26 (97% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGGGGATGG270100.13445836972736264RNA PCR Primer, Index 26 (97% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACATGAGCATCTCGTATGC265620.13222818277298062RNA PCR Primer, Index 26 (98% over 50bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC264650.13174530747258986RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATCTCGTATGCCGT260320.1295897919564126RNA PCR Primer, Index 26 (97% over 39bp)
TGGAATTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCCG257040.12795697650766863RNA PCR Primer, Index 26 (100% over 44bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA255440.12716048116681788RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGGGTATGC250050.124477287487327RNA PCR Primer, Index 26 (97% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATTC244830.12187872143780154RNA PCR Primer, Index 26 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC230850.11491934339711835RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC230150.11457087668549615RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGCGGATGC229920.11445638048024886RNA PCR Primer, Index 26 (97% over 45bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA229060.11402826423454158RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA227010.11300775457907661RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTTTGC226460.11273395930565917RNA PCR Primer, Index 26 (98% over 50bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC219270.10915470836771124RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGACACATGAGCATCTCGTATGC218820.10893069405309697RNA PCR Primer, Index 26 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA218570.10880624165608904RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACACCAGTCACATGAGCATCTCGTATGC216120.10758660816541138RNA PCR Primer, Index 26 (98% over 50bp)
TGGAATTCTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTAT207400.10324570855777494RNA PCR Primer, Index 26 (100% over 42bp)
TTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGTGCCAAGGAACTCC206430.10276283325738418RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCAGTCACATGAGCATCTCGTATGCC206420.10275785516150387RNA PCR Primer, Index 1 (96% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG203040.1010752587539567RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA201060.10008959576965394RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA207.932983E-569.9978851
CGCTATA301.0254007E-769.99753658
TATTGCA936000.069.871011
TCAGTGC103800.069.848241
TTCAACG439900.069.842765
ACGCTGT441150.069.84069
CAACGCT439350.069.839437
GATAGGC383100.069.818315
TCAACGC439450.069.8117756
TGAGGTA246500.069.803841
CGCTGTC444500.069.7952510
TTCAAGT431950.069.789941
AACATTC494450.069.7832261
AACGCTG441550.069.777338
GGATAGG441250.069.7711614
CGTAAAT99000.069.75189
ATTCAAC486750.069.7358254
ATAGTTT147900.069.7166917
AAGTAAT442100.069.677294
GTAGTAG267900.069.675265