FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22000790
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC483119521.959188738222583RNA PCR Primer, Index 25 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC9728044.421677585213986RNA PCR Primer, Index 5 (100% over 35bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT9307044.230320820297816RNA PCR Primer, Index 1 (100% over 30bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG7734063.51535558495854RNA PCR Primer, Index 1 (100% over 29bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA6676833.034813749869891RNA PCR Primer, Index 1 (100% over 34bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC6657963.0262367851336247RNA PCR Primer, Index 1 (100% over 33bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5953782.7061664603861955RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA4469112.0313406927660327RNA PCR Primer, Index 1 (100% over 34bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT3355841.5253270450742906RNA PCR Primer, Index 8 (100% over 36bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3054281.3882592397818443RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC2971611.3506833163718213RNA PCR Primer, Index 5 (100% over 35bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA2424241.1018877049415043RNA PCR Primer, Index 1 (100% over 28bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC2340501.0638254353593666RNA PCR Primer, Index 1 (100% over 27bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2137430.9715242043581162RNA PCR Primer, Index 1 (100% over 31bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2012330.9146626098426467RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1881940.8553965562145722RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT1784500.8111072375128348RNA PCR Primer, Index 8 (100% over 36bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1701460.7733631383236693RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1247390.5669750949852256RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1126120.5118543470484469RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1012050.4600062088679543RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT987370.44878842987001827RNA PCR Primer, Index 25 (100% over 39bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT984080.44729302902304874RNA PCR Primer, Index 1 (100% over 30bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG955990.4345253056822051RNA PCR Primer, Index 5 (97% over 37bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA951630.4325435586631207RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA924580.42024854562040725RNA PCR Primer, Index 25 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA911610.4143533027677643RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC873390.3969811993114793RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG821640.37345931668817345No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA800620.36390511431634953RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA782050.3554645083199285RNA PCR Primer, Index 1 (100% over 28bp)
CAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG760030.34545577681528705RNA PCR Primer, Index 5 (97% over 37bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT738640.33573339866432067RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC677460.3079253063185458RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC655700.29803475238843696RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC632340.28741695184582006RNA PCR Primer, Index 1 (100% over 33bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA623770.28352163717757406RNA PCR Primer, Index 1 (100% over 28bp)
TCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTC569600.2588997940528499RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGACACACTGATATCTCGTATGC546500.2484001710847656RNA PCR Primer, Index 25 (98% over 50bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA543660.2471093083475639RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC535290.24330489950588138RNA PCR Primer, Index 1 (100% over 27bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC524100.23821871850965354RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC491310.22331470824456756RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC456580.2075289114618157RNA PCR Primer, Index 25 (98% over 50bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA446420.20291089547238983RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT428480.19475664282964383RNA PCR Primer, Index 1 (100% over 30bp)
GTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT413050.18774325831026978RNA PCR Primer, Index 25 (100% over 39bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC390820.17763907568773665RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA369660.1680212392373183RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAATGATATCTCGTATGC365810.1662713020759709RNA PCR Primer, Index 25 (98% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA364980.1658940428957324No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC317660.14438572433080812RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC312870.1422085297846123RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA309970.1408903952994415RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGATATCTCGTATGC308020.1400040634904474RNA PCR Primer, Index 25 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTGATATCTCGTATGC307530.1397813442153668RNA PCR Primer, Index 25 (98% over 50bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA295760.13443153632210478RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCCG288580.13116801714847512RNA PCR Primer, Index 25 (100% over 44bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG279660.12711361728374299RNA PCR Primer, Index 1 (100% over 29bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC277940.1263318271752969RNA PCR Primer, Index 25 (98% over 50bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC275930.12541822361833369RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC275330.1251455061386432RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTGATATCTCGTATGC274160.12461370705324673RNA PCR Primer, Index 25 (98% over 50bp)
GGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTAT272490.1238546434014415RNA PCR Primer, Index 25 (100% over 48bp)
TGGAATTCTCGGGTGCCAAGGAACACCAGTCACACTGATATCTCGTATGC269710.12259105241220884RNA PCR Primer, Index 25 (98% over 50bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTAT266120.12095929282539401RNA PCR Primer, Index 25 (100% over 48bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC263250.11965479421420776RNA PCR Primer, Index 25 (98% over 50bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT262490.11930935207326646RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG256760.11670490014222215RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG244870.11130054875302205RNA PCR Primer, Index 1 (100% over 29bp)
TTCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACT230050.10456442700466666Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCTCAGTTCAGCAGGAACAGTTGGAATTCTCGGGTGCCAAGGAACTCC221760.10079638049360953RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGC1150.070.0142751
ACATACG455.456968E-1270.014271
GCTTACC150.002225107770.0026514
TTGTGTA252.8421455E-670.00233513
TATTCGC207.931031E-570.0013915
GTACGCG401.3460522E-1070.0013915
TATTGCA530850.069.9351351
TGTAAAC188600.069.921461
TAGCAGC244500.069.856771
TCAACGC224300.069.8448646
CCTACAC67400.069.84410
TTCAAGT526350.069.83471
GCAGCAC246550.069.816193
CGCTGTC227600.069.8152610
ACGCTGT226400.069.813649
TGAGGTA365200.069.8129651
CAACGCT224700.069.795237
ATAGTTT236650.069.7935317
TTGTATA311050.069.7772813
CAGCACG231550.069.774014
Produced by FastQC (version 0.11.5)