FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14951386
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA13647699.128043380058545RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA4739143.169699451274952RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3660162.4480406030584723RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC3654642.4443486376446972RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1978871.323536159122639RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1512001.0112774829035915RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC1440440.9634156993873344RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1421110.9504871320959809RNA PCR Primer, Index 1 (100% over 32bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1191930.7972036839929089RNA PCR Primer, Index 1 (100% over 22bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAAT1123980.7517563923505153RNA PCR Primer, Index 37 (100% over 39bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1102550.7374232729995734RNA PCR Primer, Index 1 (100% over 23bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1047690.7007310225286137RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1035430.6925311138378744RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1023340.6844449069805301RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1019610.6819501549889756RNA PCR Primer, Index 2 (100% over 34bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC848680.5676263056816271RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA811830.5429797612074225RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA783290.5238912298833032RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA725250.4850720862935383RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT713970.4775276352306067RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA703730.47067877185432844RNA PCR Primer, Index 1 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG666430.44573125193878343Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC657390.43968498973941283RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA647420.43301671162793876RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC638180.4268366825657501RNA PCR Primer, Index 2 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC625790.418549825414179RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAA605710.40511963238725823RNA PCR Primer, Index 37 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC604970.40462469499483195RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG547930.36647438571915675RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA494760.3309124652390086RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG491880.3289862224144304RNA PCR Primer, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG488900.3269930961584431No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA479220.320518779998055RNA PCR Primer, Index 7 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC465910.31161659527752145RNA PCR Primer, Index 37 (100% over 50bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG453790.30351032339075457No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC447510.29931004389827137RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC434670.2907222113053599RNA PCR Primer, Index 1 (100% over 33bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC434490.29060182112882377RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG418570.2799539788485161RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC416160.27834208815156003RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC404050.27024250460793403RNA PCR Primer, Index 2 (100% over 34bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG392570.2625642866821845RNA PCR Primer, Index 2 (100% over 35bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG389390.2604373935633793RNA PCR Primer, Index 7 (97% over 36bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA380890.2547523018936171No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA373220.24962234270454928RNA PCR Primer, Index 1 (100% over 32bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG369410.2470740839678676RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC365280.24431179825067725RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA353410.23637273494243274RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC350900.23469396081406768RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG345310.2309551769983064RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA339630.22715619809427703RNA PCR Primer, Index 1 (100% over 28bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATA325950.21800654467753022RNA PCR Primer, Index 37 (100% over 40bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC323320.2162475104314744RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA316890.21194690579187775RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA316650.21178638555649623RNA PCR Primer, Index 1 (100% over 32bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA308820.20654941287717404RNA PCR Primer, Index 1 (100% over 28bp)
ATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA307430.2056197331805894RNA PCR Primer, Index 7 (97% over 37bp)
TCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAA284080.19000245194659546RNA PCR Primer, Index 37 (100% over 38bp)
CATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG274590.18365521430588444RNA PCR Primer, Index 7 (97% over 36bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATAT270790.18111364391234364RNA PCR Primer, Index 37 (100% over 41bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG269710.1803913028531268RNA PCR Primer, Index 2 (100% over 35bp)
CCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAAT260260.17407081858497936RNA PCR Primer, Index 37 (100% over 39bp)
TCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC256190.17134866292663437RNA PCR Primer, Index 1 (100% over 33bp)
CCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC255730.1710409991421531RNA PCR Primer, Index 2 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG254570.1702651513378091RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG248500.16620532705128474RNA PCR Primer, Index 2 (100% over 35bp)
CCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG244620.1636102499126168RNA PCR Primer, Index 2 (100% over 35bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA242440.1621521911079013RNA PCR Primer, Index 1 (100% over 32bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC240870.16110212123478052RNA PCR Primer, Index 2 (100% over 34bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG238910.15979120597916474Illumina Small RNA Adapter 2 (100% over 21bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT236570.15822613368419491No Hit
TTTCACCGGCTCTGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC235320.1573900907915828RNA PCR Primer, Index 1 (100% over 33bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAAT230310.1540392308779935RNA PCR Primer, Index 37 (100% over 39bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG229400.1534305916521719RNA PCR Primer, Index 2 (100% over 35bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG228880.1530827978088453RNA PCR Primer, Index 7 (97% over 36bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA226340.1513839586510575RNA PCR Primer, Index 1 (100% over 22bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC221980.14846784104162652RNA PCR Primer, Index 1 (100% over 27bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC220320.14735757608023764No Hit
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG218430.14609347922660817RNA PCR Primer, Index 2 (100% over 35bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC218150.14590620561866305RNA PCR Primer, Index 2 (100% over 34bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC218010.14581256881469049RNA PCR Primer, Index 1 (100% over 33bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATA217530.14549152834392745RNA PCR Primer, Index 37 (100% over 40bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC212810.1423346303814242RNA PCR Primer, Index 1 (100% over 26bp)
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG211890.14171930281246167RNA PCR Primer, Index 7 (97% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG211650.14155878257708013RNA PCR Primer, Index 7 (97% over 36bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG206900.13838181958515416Illumina Small RNA Adapter 2 (100% over 21bp)
GTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCT205280.1372983079963289RNA PCR Primer, Index 37 (100% over 43bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC200190.13389394133761245RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA199010.13310471684698663RNA PCR Primer, Index 1 (100% over 28bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATA198280.13261646779770117RNA PCR Primer, Index 37 (100% over 40bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC197750.13226198561123365RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA197480.13208140034642943No Hit
CCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG193490.12941275143321163RNA PCR Primer, Index 2 (100% over 35bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA193130.1291719710801393RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA191970.1283961232757953No Hit
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC191730.1282356030404138RNA PCR Primer, Index 1 (100% over 27bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG191180.12786774416766444Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA186820.12495162655823346RNA PCR Primer, Index 1 (100% over 28bp)
GTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA186320.12461720940118862RNA PCR Primer, Index 7 (97% over 37bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA185930.12435636401869365No Hit
CCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC178030.1190725729373852RNA PCR Primer, Index 2 (100% over 34bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA177450.11868464903521318RNA PCR Primer, Index 1 (100% over 28bp)
CCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC177240.11854419382925437RNA PCR Primer, Index 2 (100% over 34bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG177020.11839705028015464RNA PCR Primer, Index 1 (100% over 29bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAA174770.11689217307345286RNA PCR Primer, Index 37 (100% over 38bp)
GTAGTCGTGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA174170.11649087248499905RNA PCR Primer, Index 7 (97% over 37bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC173970.11635710562218113RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT171360.11461144806240707RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC169980.1136884567089633RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG168580.11275208866923776RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG166470.11134084826650854No Hit
CCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG165780.11087935258978665RNA PCR Primer, Index 2 (100% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT165690.11081915750151859RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA161650.10811706687259628RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC158430.10596342038122752RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC157130.10509393577291094RNA PCR Primer, Index 1 (100% over 27bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG154840.10356230519364558No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG154400.10326801809544613RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA151330.10121469675119082RNA PCR Primer, Index 1 (100% over 28bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT150860.10090034462356867No Hit
GTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAA150210.10046560231941037RNA PCR Primer, Index 37 (100% over 38bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG149850.10022482196633811RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph