FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006135121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9817219
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG3403153.46651123907901RNA PCR Primer, Index 15 (97% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1846941.8813270845847485RNA PCR Primer, Index 6 (100% over 34bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1757291.790007944204973RNA PCR Primer, Index 1 (100% over 32bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1460161.4873458562959634RNA PCR Primer, Index 1 (97% over 35bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1107221.127834674972617RNA PCR Primer, Index 1 (100% over 30bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1087311.1075539824465563RNA PCR Primer, Index 1 (100% over 23bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1008741.0275211340400983RNA PCR Primer, Index 15 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA985441.003787325107039RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC976600.9947827383702045RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGC820420.8356949152300668RNA PCR Primer, Index 19 (100% over 50bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC785060.7996765682827286RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA768470.7827776888750266RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA747260.7611727924170786RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA725480.7389872834659185RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT720480.7338941914201975RNA PCR Primer, Index 1 (97% over 35bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC701850.7149173304578414RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC696500.7094677219689202RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA682570.6952783675295418RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC672300.684817156467631RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC653180.6653411724847944RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT627790.6394784510766236RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA617500.6289968676465301RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC601350.6125461803388516RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC598960.610111682340997RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA572030.5826802885827442RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC534670.5446247048171178RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA504180.5135670295223118RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA500480.5097981414084782RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA484530.4935511777826287RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC457390.46590587415845564RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA454770.4632370939264979RNA PCR Primer, Index 19 (100% over 39bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG452950.4613832084218555RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA429160.43715027646831556RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC396350.403729406464295RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC375780.38277642578819926RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG371140.3780500363697703RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA352900.35947043658698047RNA PCR Primer, Index 1 (100% over 32bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA348220.35470330243218573RNA PCR Primer, Index 19 (100% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG314010.31985636665536343RNA PCR Primer, Index 6 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC308100.3138363318573213RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA305080.3107601042617059RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC300100.3056873845841679RNA PCR Primer, Index 1 (100% over 33bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG278790.2839806262853054No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG274700.27981447699190576RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT273020.27810319806454353RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA267720.2727045204960794RNA PCR Primer, Index 1 (100% over 22bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG264880.2698116442141099RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG260390.26523804755705255RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA258000.262803549559198RNA PCR Primer, Index 19 (100% over 37bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA257100.26188679299096823RNA PCR Primer, Index 1 (100% over 32bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG228570.2328256097780848RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA213410.21738335469545905RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA205290.20911217321320832RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA201970.20573036009484966RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT200440.2041718739288591RNA PCR Primer, Index 1 (100% over 30bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA198810.2025115259219541RNA PCR Primer, Index 19 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG194720.19834537662855437RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA191420.1949839358783786No Hit
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA189940.1934763806328452RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG186160.18962600304628022RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC182130.18552097085742916RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG174280.17752481634564737RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC170060.17322624665905895RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC167990.1711177065521305RNA PCR Primer, Index 1 (100% over 33bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC167230.17034355656118091RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC166820.16992592301343182RNA PCR Primer, Index 19 (100% over 42bp)
TACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT166580.1696814545952372RNA PCR Primer, Index 1 (100% over 30bp)
ATCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA165150.16822483027016102RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC163100.16613666253141546RNA PCR Primer, Index 1 (100% over 33bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA162050.16506711320181408RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG159170.16213349218347886RNA PCR Primer, Index 15 (97% over 36bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC158270.16121673561524907RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC156500.1594137810310639RNA PCR Primer, Index 1 (100% over 33bp)
CTCCTGGCTGGCTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA153750.1566125804059174RNA PCR Primer, Index 1 (100% over 32bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG152440.15527819028993853No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA150760.1535669113625763RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG149740.15252792058524925RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA146500.1492275969396221RNA PCR Primer, Index 19 (100% over 38bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA142400.145051261462131RNA PCR Primer, Index 1 (100% over 22bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA139810.14241303978244757RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC138680.14126200098011463RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA138090.14066101611871956RNA PCR Primer, Index 19 (100% over 38bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC132500.13496693921160363RNA PCR Primer, Index 1 (100% over 27bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA131780.13423353395701978RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT127370.12974142677269399RNA PCR Primer, Index 1 (97% over 35bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAT126920.1292830484885791RNA PCR Primer, Index 19 (100% over 41bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG126090.12843759520898942Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA125460.1277958656112286No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG125170.12750046627257677Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC124650.12697078469982181RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC121810.12407790841785235RNA PCR Primer, Index 1 (100% over 27bp)
CCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT120310.12254998080413607RNA PCR Primer, Index 1 (97% over 35bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG120270.12250923606777031Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA116950.11912742294941164RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAT116790.11896444400394857RNA PCR Primer, Index 19 (100% over 41bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT112090.11417693748097092RNA PCR Primer, Index 1 (97% over 35bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC111330.11340278749002136RNA PCR Primer, Index 1 (100% over 27bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG111290.11336204275365559RNA PCR Primer, Index 15 (97% over 36bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA109370.11140629540809877RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC108680.1107034487057893RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC103970.1059057559987202RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACT103420.1053455158736909RNA PCR Primer, Index 1 (100% over 25bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC102190.10409261523044358RNA PCR Primer, Index 1 (100% over 33bp)
ATCTCGGTGGGACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA101440.10332865142358544RNA PCR Primer, Index 1 (100% over 32bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAA99620.10147476591894303RNA PCR Primer, Index 19 (100% over 40bp)
CCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG99180.1010265738189196RNA PCR Primer, Index 6 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA98870.1007108021120849RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph