FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8664107
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA3189863.6816950667853017RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2949863.404690177533588RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2239282.5845479516815755RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2162442.4958602196394852RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1917712.213396025695435RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1601231.8481189117355084RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1504961.7370053255344144RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1368571.5795857553467425RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1240081.4312842627636062RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1006721.1619431754478562RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG925031.0676576362688042RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA882711.0188124407974186RNA PCR Primer, Index 1 (100% over 22bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC850840.9820284998788681RNA PCR Primer, Index 2 (100% over 34bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG821020.9476106423893426RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC790820.9127541938251685RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC788700.910307317303445RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC733380.846457690330925RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG670480.7738593256062051RNA PCR Primer, Index 1 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG661620.7636332284446626Illumina Small RNA Adapter 2 (100% over 21bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG629500.7265607407664748Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA591040.6821707072638876RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC587570.6781656782401233RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA578100.667235526985066RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA490160.5657363188150839RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA455580.525824531022066RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG446540.5153906801935849RNA PCR Primer, Index 7 (97% over 36bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG445540.5142364931550361No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA398680.4601512885286389RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC396370.45748511646959117RNA PCR Primer, Index 1 (100% over 27bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG341040.3936239476266856Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG340930.3934969870524452Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA340060.39249284432890774RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG326500.3768420680861859RNA PCR Primer, Index 1 (100% over 29bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG320690.37013624139221735Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG297930.34386694439484644RNA PCR Primer, Index 1 (100% over 29bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA297760.34367073259829317RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC291820.33681486158931323RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG289540.33418331514142197RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA286290.3304322072661383RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC273740.3159471599323508RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA266290.3073484664951622RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC261070.3013236101539374RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG251280.29002411904654457RNA PCR Primer, Index 2 (100% over 35bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT250710.2893662324345717No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC243360.280882957701238RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG242490.27987881497770056RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA238840.2756660322869974No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAAT235990.27237659922713325RNA PCR Primer, Index 37 (100% over 39bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC222840.25719903967021646RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG218570.2522706610156131RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC206950.23885900762767587No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC203380.23473855990005663RNA PCR Primer, Index 1 (100% over 27bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG201550.23262639761951232Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA200900.23187617604445557RNA PCR Primer, Index 1 (100% over 28bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA198020.22855211737343503RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT193460.22328902447765245RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT174540.20145180570830898RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC166910.1926453586041816RNA PCR Primer, Index 37 (100% over 50bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC165910.19149117156563278RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG165120.19057936380517923Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC162760.18785548239420405RNA PCR Primer, Index 1 (100% over 33bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC162390.18742843318994099No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA160220.18492384731629005RNA PCR Primer, Index 7 (97% over 37bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA159020.1835388228700315RNA PCR Primer, Index 1 (100% over 23bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG151430.17477854324744604No Hit
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC150210.1733704350604165RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA148190.1710389772425479No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA147290.17000020890785397No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA145190.16757641612690147RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG141160.16292504236154978RNA PCR Primer, Index 7 (97% over 36bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC141090.16284424926885135RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA140580.16225561387919146RNA PCR Primer, Index 1 (100% over 23bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG138660.16003957476517777No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC138150.15945093937551785RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA137640.15886230398585796RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC136080.15706177220572184RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC135780.15671551609415718RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA134080.1547533981286242RNA PCR Primer, Index 1 (100% over 32bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA132060.15242194031075562RNA PCR Primer, Index 1 (100% over 23bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC131490.15176405369878282RNA PCR Primer, Index 2 (100% over 34bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC127200.1468125913034084RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG125920.14533523189406594RNA PCR Primer, Index 2 (100% over 35bp)
GTTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGA119330.13772913931002928RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG117980.1361709868079884RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA117890.136067109974519RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC117120.13517838595483644RNA PCR Primer, Index 1 (100% over 33bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC116960.1349937160286686RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG116700.13469362739864593RNA PCR Primer, Index 2 (100% over 35bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC115940.13381644524934885RNA PCR Primer, Index 1 (100% over 33bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA115210.13297388871120822RNA PCR Primer, Index 1 (100% over 23bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG113420.13090789391220584Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC109560.12645273194340745RNA PCR Primer, Index 1 (100% over 26bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC107460.12402893916245496No Hit
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTCGGGTGCCAAGGAA105800.12211298867846393RNA PCR Primer, Index 1 (100% over 23bp)
AGTAATGATGAATGCCAACCGCTCTGATTGGAATTCTCGGGTGCCAAGGA103800.11980461460136631RNA PCR Primer, Index 1 (100% over 22bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAA102640.11846575763664968RNA PCR Primer, Index 37 (100% over 38bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA100870.1164228465784183RNA PCR Primer, Index 1 (100% over 32bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG99820.11521095018794206RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG99410.11473773350213705RNA PCR Primer, Index 7 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT97200.11218698014694417RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA96050.11085966505261305RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG95130.10979781297714815RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT94900.10953234995828191RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC93990.10848203975320249RNA PCR Primer, Index 1 (100% over 31bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG93250.10762794134467636No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG90470.10441930137751068RNA PCR Primer, Index 1 (100% over 29bp)
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGGGTGCCAA89930.10379604037669433No Hit
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG88870.10257260211583259RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC87520.10101444961379172RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph