FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12663414
Sequences flagged as poor quality0
Sequence length2-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG273510.21598440989136106No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG184710.1458611398158506No Hit
GGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCG173650.13712731811500437No Hit
GGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGG128360.10136287102356441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG81750.064.24664145
AGTCCGC108450.045.3814937
CAGTCCG114200.042.6256336
ACTCACG71400.038.51475145
GTCCGCC138050.037.304498
TCCGCCC148050.035.91959
GACAGTC136000.032.34524
ATAACAC91350.032.0029687
ACAGTCC139100.031.4764945
TTTCCGA235000.029.1319947
TGTCGAG40200.028.3588319
TTCCGAC246300.027.9875588
TCGCGGG172000.027.583075145
TCCGACC266500.027.0002639
GCACCGT55050.026.952441
ATTATGC100600.026.9289688
GTTTCCG239100.026.6967076
CCATAAC95850.026.6522065
GCTATGT89050.026.363052
GGACAGT172950.026.1326963
Produced by FastQC (version 0.11.5)