FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16194197
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG1047470.6468181163907046No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT623930.38527998640500666No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG292390.1805523299488082No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG220190.1359684583310923No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT208140.128527521309022No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA192820.11906734245606619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG243250.0127.424144
TACGGCC247950.0125.213457
GGTCTAC217200.0117.786243
TCTACGG263700.0117.514875
CTACGGC274750.0113.158066
ACGGCCA292600.0106.428348
CGGCCAT320350.097.3675169
GGGTCTA242100.093.880472
CGGTCTA42500.086.6529852
GCGTCTA51600.082.914422
CGTCTAC71150.079.881073
GCGCGGT263500.078.090869
CTTAGCG151500.062.7420239
CGGGTAT34150.057.0759431
GGGGTCT404300.054.5244371
CGGTTCA57850.054.3944745
GGGGTAC80350.054.309321
CGGGTAC38450.053.908541
GGGGGTT277050.053.1063841
GGGGGAA435100.053.105211
Produced by FastQC (version 0.11.5)