FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16065740
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA9526665.929798440656951RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC8835835.499796461289676RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA4162972.591209617484162No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC4095502.5492134193631917RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3517982.1897404041145943RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2886451.7966492673228873RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2862901.781990745524327RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2737861.7041605304206342RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2587311.6104518061415158RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2441871.5199237632377967RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2134701.3287280884665131RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1805561.1238573511086325RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1598930.9952420492302253RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1504530.9364834735281413RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1472330.9164408237653542RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1417390.8822438306607725RNA PCR Primer, Index 1 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1401170.8721478126746729No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC1265950.7879811325217513RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA1257360.7826343511098772RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1253070.7799640726166364RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA1214660.7560560546853118RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1214060.7556825891617815RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1181920.7356772859513474RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC1146830.7138357772502231RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1081790.67335211449955RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1051740.6546477161960794RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1028380.6401074584799704RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC909700.5662359779256978RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC824040.5129175500163703RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC817930.5091144261017544RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC800700.4983897411510456RNA PCR Primer, Index 2 (100% over 34bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA789490.4914121602864231RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC773750.48161491471914775RNA PCR Primer, Index 1 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT747620.46535049116940774RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA704390.4384423001990571RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA642740.40006871765632956RNA PCR Primer, Index 1 (100% over 22bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC631210.39289195517915765RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA590260.36740293319822176RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT576080.3585766979921248RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG562810.35031688549671536RNA PCR Primer, Index 7 (100% over 36bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA561660.3496010765766158RNA PCR Primer, Index 1 (100% over 23bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC559990.3485615975361235RNA PCR Primer, Index 1 (100% over 33bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC501230.311986873931733RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG498840.31049923626300435RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC488410.30400716057897115No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA479560.29849854410690074RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA473390.29465807363993196RNA PCR Primer, Index 1 (100% over 22bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG451820.2812319880690214RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA446970.278213141753819No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC432230.26903833872576055RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA417840.26008139058642804RNA PCR Primer, Index 1 (100% over 22bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT413890.2576227425565209RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT410040.25522633878053547RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG399080.24840436855071724RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA399060.2483919196999329RNA PCR Primer, Index 7 (100% over 35bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC394690.24567184580355467RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA387330.24109066871491758RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG375250.2335715628411763RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC354340.22055628934614901RNA PCR Primer, Index 1 (100% over 27bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA339070.21105159177230554RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC331510.20634592617582506RNA PCR Primer, Index 1 (100% over 26bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA327100.20360095457787816RNA PCR Primer, Index 1 (100% over 23bp)
TGTCAGTTTGTCAAATACCCCATTGGAATTCTCGGGTGCCAAGGAACTCC322720.20087465625610773RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG312420.1944634981021727RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306430.19073506729226292No Hit
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC300010.18673898619048981RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG299430.186377969517744RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG299120.1861850123305867RNA PCR Primer, Index 1 (100% over 29bp)
AGTTGGTCCGAGGGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA294760.1834711628596006No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC294700.1834338163072476RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA288840.17978630302743603RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTTGGAATTCTCGGGTGCCAAGGAACT264120.16439952345799197RNA PCR Primer, Index 1 (100% over 25bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC264050.1643559524802468RNA PCR Primer, Index 1 (100% over 27bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC263360.16392646712818706RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG260120.161909753301124RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC259380.16144914582210343RNA PCR Primer, Index 1 (100% over 27bp)
TTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAAC257160.1600673233850417RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC252620.15724143425699658RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA252610.1572352098316044RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG241480.15030742437011926Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC237190.14763714587687837RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG233390.14527186422785382No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA228120.14199159204618025No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC226350.14088986875176618RNA PCR Primer, Index 1 (100% over 24bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA219460.1366012396565611RNA PCR Primer, Index 1 (100% over 32bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA215800.1343230999630269No Hit
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA208280.12964233206811512RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA203890.12690980932095253RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA203190.12647409954350064RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA202890.12628736678173555No Hit
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA202870.1262749179309512RNA PCR Primer, Index 1 (100% over 32bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA202610.1261130828707548RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC196100.12206098194045217RNA PCR Primer, Index 1 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG194390.12099660519839112RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT193650.12053599771937053RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG191870.11942804999956427RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAAC191450.11916662413309315RNA PCR Primer, Index 1 (100% over 24bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG190460.11855040601926833RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG189250.11779725054681578Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA188640.1174175605978934RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA188300.11720593013455963RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC183810.11441116313347532RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC177230.11031549122542753RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT174760.10877805815356155RNA PCR Primer, Index 1 (100% over 25bp)
AGCTCGGTCTGAGGCCCCTCAGTTTGGAATTCTCGGGTGCCAAGGAACTC174440.10857887654101211RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG174390.10854775441405126RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA172450.1073402158879703RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA171220.1065746115647334RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACATGGAATTCTCGGGTGCCAAGGAACTCC170680.10623849259355624RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA163640.10185649711746861RNA PCR Primer, Index 1 (100% over 32bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA162250.10099130198795699RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC161690.10064273416599548RNA PCR Primer, Index 1 (100% over 26bp)
TTCACCACCTTCTCCACCCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA161650.10061783646442679RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCA1302050.069.901681
CAACGGA1089400.069.875961
CAACGCT666200.069.87587
TCAGTGC443500.069.859761
TTCAAGT1362350.069.8535461
TTCAACG666750.069.853325
AACATTC693900.069.816461
GCACGTA419400.069.813616
TAGCAGC445450.069.80551
ACGTAAA419750.069.8048558
CGCTGTC667450.069.80282610
ATTCAAC687050.069.801774
CATTCAA686900.069.798153
CAGCACG419300.069.788514
TGGTCCG783450.069.77774
ACGCTGT668150.069.77169
TCAACGC667500.069.748616
GGATAGG1383200.069.73608414
TAATCCA1148350.069.7223367
CTGGTCC694600.069.719843