FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006135168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10252175
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC661643764.53691046046328RNA PCR Primer, Index 24 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGT1589521.5504222274785595RNA PCR Primer, Index 9 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATATCGTATGC626250.610845991216498RNA PCR Primer, Index 24 (98% over 50bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT592460.5778871312672677RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCAGCTCGTATGC569150.5551504924564787RNA PCR Primer, Index 24 (98% over 50bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT536650.5234499020939459RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCGCGTATGC495890.48369248476542787RNA PCR Primer, Index 24 (98% over 50bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG492850.48072726031305557RNA PCR Primer, Index 6 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGGATCTCGTATGC436450.42571454349930626RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTAGTATGC401380.3915071679911824RNA PCR Primer, Index 24 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC393290.38361615949786265RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGG379710.37037018974022584RNA PCR Primer, Index 24 (97% over 49bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGT378000.3687022509857664RNA PCR Primer, Index 9 (97% over 36bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG371920.36277180208102183RNA PCR Primer, Index 2 (97% over 35bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA354860.34613143064764307RNA PCR Primer, Index 1 (100% over 32bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC341380.33298300116804486RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTTTGC330090.32197070377749115RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATTC329270.3211708734975749RNA PCR Primer, Index 24 (98% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG297050.2897433959135501RNA PCR Primer, Index 6 (100% over 34bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG295840.28856315854928344RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTAAGC286980.2799210899150668RNA PCR Primer, Index 24 (98% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG283080.27611701907156283RNA PCR Primer, Index 2 (97% over 35bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA263360.2568820762423583RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGA261090.254667911930883RNA PCR Primer, Index 24 (97% over 49bp)
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC255640.24935196677778132RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATGTCGTATGC216770.21143806070419205RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGTAGCATCTCGTATGCC212310.20708776430367215RNA PCR Primer, Index 24 (97% over 35bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG209800.20463950332490424RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206280.20120608553794683No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGAATCTCGTATGC182430.17794272922574966RNA PCR Primer, Index 24 (98% over 50bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA173330.16906656392424046RNA PCR Primer, Index 1 (100% over 28bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC166240.16215095821130637RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTGGTATGC152880.14911957706535442RNA PCR Primer, Index 24 (98% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA147290.1436670755229988RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC141600.13811703370260456RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACGGTAGCATCTCGTATGCC140660.13720015508904207RNA PCR Primer, Index 24 (96% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA138070.13467386188784333RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGTAGCATCTCGTATGCC137780.1343909950815315RNA PCR Primer, Index 24 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA136780.13341559230114586RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA126570.12345672991340861RNA PCR Primer, Index 1 (100% over 28bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGCC122850.11982823157037409RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGGATGC122680.11966241309770853RNA PCR Primer, Index 24 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC120900.11792619614862211RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG118710.11579006405957759RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGC117880.11498047975185754RNA PCR Primer, Index 24 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACACCAGTCACGGTAGCATCTCGTATGC117830.11493170961283826RNA PCR Primer, Index 24 (98% over 50bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA116400.11353688363688681RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATAGCATCTCGTATGC113530.11073747765718005RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTTGCATCTCGTATGC113220.11043510279526052RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCACGTATGC111040.10830872473401987RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGAATGC108240.1055775969489401RNA PCR Primer, Index 24 (98% over 50bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGCC104860.10228073555123669RNA PCR Primer, Index 24 (97% over 41bp)
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACGGTAGCATCTCGTATGC103070.1005347645743464RNA PCR Primer, Index 24 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)