FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7255871
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT337720.4654437764949239No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG203600.2806003579721856No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA158790.21884347172103805No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT119000.16400512081871357No Hit
GGGGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG79850.11004881426364939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG56500.0115.624214
TACGGCC60000.0108.758617
TCTACGG64500.0101.1708155
GGTCTAC59050.088.7597353
GCGCGGT134650.081.794459
CTACGGC85500.076.576256
ACGGCCA88000.075.059938
CGGTTCA33300.073.594425
CGGCCAT89850.073.514469
GGGTCTA66250.071.8141252
GCCGGTT36150.067.981233
CGGTCTA13550.061.6471442
GGGGGAT158450.060.8619421
CCGGTTC42100.058.5557144
CGGGGAT52850.057.3833121
GGGGGAG399300.057.3363381
GGGGGGA324600.056.6175771
CGGCACT32450.056.2540251
CCGGGCG198450.055.4616935
GCGTCTA20400.055.1894342
Produced by FastQC (version 0.11.5)