FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091706
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC5784394.783766657905841RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA5642494.666413490371004RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC5528804.572390364105776RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA5143854.254031647808837No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC4698913.886060411988184RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2447082.023767365829106RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1922151.5896433472662996RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1634271.351562798500063RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1301681.0765064913090014RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1290001.0668469775894318No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA1221001.0097830694858112RNA PCR Primer, Index 1 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1199180.9917376423144922RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1161100.960244981146581RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1150770.9517019351942564RNA PCR Primer, Index 2 (100% over 34bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1122350.9281982211608518RNA PCR Primer, Index 1 (100% over 22bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1088970.9005925218492743RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1080050.8932155644538496RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1030070.851881446670966RNA PCR Primer, Index 1 (100% over 30bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1028930.8509386516675149RNA PCR Primer, Index 1 (100% over 23bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA780790.6457236059163198RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA738180.6104845751294317RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA683160.564982311015501RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT672690.5563234832206473RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG653080.5401057551349661RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT630990.5218370344101982RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA629250.5203980315101939RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT627410.5188763272940973RNA PCR Primer, Index 9 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA609300.5038991189497991RNA PCR Primer, Index 7 (100% over 35bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC592440.48995567705665355RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC580500.4800811399152444RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC563830.46629483052267395RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC556190.45997644997322956No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA553130.4574457897008081RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC531180.4392928508185694RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC474030.39202904867187477RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA467240.3864136293092141RNA PCR Primer, Index 1 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA454460.37584440111263046RNA PCR Primer, Index 1 (100% over 23bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC443500.36678033686892486RNA PCR Primer, Index 1 (100% over 33bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA425560.3519437207619835RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC420640.347874816010247RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC417680.3454268570539178RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA409560.33871151018723084RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC394870.32656268685328604RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC388430.32123672209694815RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC382970.3167212302383138RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA371760.3074504127043777No Hit
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC365350.30214925834286743RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC349930.28939671540144957RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC347310.2872299409198338RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTTGGAATTCTCGGGTGCCAAGGAACT344940.28526991972844856RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG329580.27256699757668607RNA PCR Primer, Index 1 (100% over 29bp)
TTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAAC312700.258607015420322RNA PCR Primer, Index 1 (100% over 24bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC310900.2571183917306623RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT299210.24745060787948364RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA298860.24716115327316096RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG291950.24144649233118964RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA284580.23535140533519425No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG269400.22279734555239764Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA247300.20452035469602056RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA246650.20398279614142123RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA244440.2021550970557835No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC225050.18611931186550518RNA PCR Primer, Index 1 (100% over 24bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC223350.1847133894919377RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA219490.18152111869077864RNA PCR Primer, Index 1 (100% over 23bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA218140.18040465092353386RNA PCR Primer, Index 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217670.18001595473790052No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC215900.1785521414430685RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA199950.16536128152636195RNA PCR Primer, Index 1 (100% over 32bp)
TTCACCACCTTCTCCACCCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA197100.16300429401773414RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAATGGAATTCTCGGGTGCCAAGGAACTCC193280.15984510374301195RNA PCR Primer, Index 1 (100% over 27bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA192110.15887749834473316RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA189950.15709114991714154RNA PCR Primer, Index 1 (100% over 28bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA185170.15313802700793422RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC184590.15265835937459943RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG184560.15263354897977174RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC179210.14820902856883886RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC176680.14611668527170607RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA175810.1453971838217039No Hit
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAAC171620.14193199867744055RNA PCR Primer, Index 1 (100% over 24bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA168060.1389878318245581RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC168060.1389878318245581RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT166030.13730899510788636RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGTTGGAATTCTCGGGTGCCAAGGAACT161830.13383553983201377RNA PCR Primer, Index 1 (100% over 25bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA161670.13370321772626625RNA PCR Primer, Index 1 (100% over 28bp)
TCTCGCTGGGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC159510.13191686929867463RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG159110.13158606403430584No Hit
TATTGCACTTGTCCCGGCCTGTTATGGAATTCTCGGGTGCCAAGGAACTC158090.13074251061016537RNA PCR Primer, Index 1 (100% over 26bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC157190.1299981987653355No Hit
AGCTCGGTCTGAGGCCCCTCAGTTTGGAATTCTCGGGTGCCAAGGAACTC155870.1289065413929184RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT154030.12738483717682186RNA PCR Primer, Index 1 (100% over 30bp)
TTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGTGCCAAGGAACTCC152270.12592929401359906RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT151200.12504438993141248RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC150400.12438277940267485RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC149290.1234647947940514RNA PCR Primer, Index 1 (100% over 33bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA146040.12077700202105476RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA145950.1207025708365718RNA PCR Primer, Index 1 (100% over 22bp)
AGCTCGGTCTGAGGCCCCTCAGTATGGAATTCTCGGGTGCCAAGGAACTC145800.12057851886243347RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC144900.11983420701760364RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA143390.11858541714461135No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG139210.11512850213195723Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC137870.1140203044963217RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTATTGGAATTCTCGGGTGCCAAGGAACT137020.11331734330953795RNA PCR Primer, Index 1 (100% over 25bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA136910.11322637186183654RNA PCR Primer, Index 1 (100% over 32bp)
ACCACAGGGTAGAACCACGGACTTGGAATTCTCGGGTGCCAAGGAACTCC136840.113168480940572RNA PCR Primer, Index 1 (100% over 27bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA136630.11299480817677837No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG136230.11266400291240955RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACATCATGGTTTACATGGAATTCTCGGGTGCCAAGGAACTCCA136180.11262265225436344RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATG135130.1117542884353953RNA PCR Primer, Index 12 (97% over 37bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA126410.10454273367215511RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA123450.10209477471582588RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG121970.10087079523766124RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph