FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17214580
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA11404386.624837782856161RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC7526104.371933558646218RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG6566653.8145862402684236RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA5467433.1760461190455995RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4637312.693826976899814RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3945692.2920628908750604RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3429641.9922879326710263RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3097331.7992480792444545RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2866481.665146637327196RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2617181.5203275363093378RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2245101.3041851732659175RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1761821.023446404152759RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1390860.8079546523934944RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1274150.740157471166883RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA1209850.7028054126211618RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1120240.6507507008593878RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1118840.6499374367541932RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1040870.6046444351241795RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGC1029080.597795589552577RNA PCR Primer, Index 34 (100% over 50bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT940460.5463159716937619RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA909490.5283254078809939RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA889450.5166841131180662RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA808710.4697820103656319RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC749770.43554359153694133RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG713350.4143871067432374RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC694730.4035706941441499RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC687220.39920811312271337RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC679170.3945318445178448RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC668760.388484644992791RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA643460.3737878008060609RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA615170.35735405685180816RNA PCR Primer, Index 1 (100% over 32bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA614710.35708684150295855RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA608240.3533283995310952RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG597600.3471475923316166RNA PCR Primer, Index 1 (100% over 29bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA561110.3259504443326529RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC560780.3257587463649999RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA551980.3206468005609199RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC534100.3102602561317209RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG524640.3047649143923349RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG499520.29017263273341554RNA PCR Primer, Index 1 (100% over 29bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA474910.2758766115699599RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG463740.26938792581637194RNA PCR Primer, Index 1 (100% over 29bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT463340.2691555646434592RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC449840.2613133750576546RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA445460.25876902021426024RNA PCR Primer, Index 1 (100% over 28bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT443790.2577989123173496RNA PCR Primer, Index 1 (100% over 30bp)
TCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC440270.25575413399571756RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC429030.2492247850368699RNA PCR Primer, Index 1 (100% over 26bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA423530.2460298189093199RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA402990.23409807268025126RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC401800.23340679819083587RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG400570.23269228758412927RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC399680.23217528397439843RNA PCR Primer, Index 1 (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG393230.2284284600611807No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC384170.22316547949470739RNA PCR Primer, Index 1 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA374930.21779793640042336No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC365470.21230259466103732RNA PCR Primer, Index 1 (100% over 27bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA361510.21000221904920133RNA PCR Primer, Index 1 (100% over 28bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG354490.20592428046458294RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG343810.1997202371478131RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA340100.19756508726904753RNA PCR Primer, Index 1 (100% over 32bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA335790.19506139563091285RNA PCR Primer, Index 1 (100% over 23bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG326520.1896764254486604RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA324000.18821255005931017RNA PCR Primer, Index 7 (100% over 35bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG317800.18461095187916288RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC311500.18095126340578743RNA PCR Primer, Index 1 (100% over 31bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC307590.1786799329405655RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC304710.17700693249559385RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA297460.17279538623655064RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA288300.16747431537684918RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC282380.16403537001774077RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG281760.16367521019972606RNA PCR Primer, Index 1 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC279730.16249597724719395RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTATGGAATTCTCGGGTGCCAAGGAACTCCA270900.15736660435514546RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT269270.15641973257552608RNA PCR Primer, Index 1 (100% over 30bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC268640.15605376372818855RNA PCR Primer, Index 2 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC264700.15376500617499816RNA PCR Primer, Index 1 (100% over 27bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC261320.1518015542638856RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC260970.15159823823758697RNA PCR Primer, Index 1 (100% over 27bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA258570.15020407120011062RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC250510.1455219935659191RNA PCR Primer, Index 2 (100% over 34bp)
TTCAAGTAATTCAGGATAGGTTATGGAATTCTCGGGTGCCAAGGAACTCC250230.14535934074488022RNA PCR Primer, Index 1 (100% over 27bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA246990.1434772152442871RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC238300.1384291687627581RNA PCR Primer, Index 1 (100% over 27bp)
TCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC235570.1368433037576287RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC231360.13439770241272225RNA PCR Primer, Index 2 (100% over 34bp)
CACCTTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC229140.13310809790305658RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG227670.1322541705926023RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA226190.1313944342528252RNA PCR Primer, Index 1 (100% over 32bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA224850.1306160243235676RNA PCR Primer, Index 1 (100% over 22bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCA223670.12993055886347502RNA PCR Primer, Index 1 (100% over 28bp)
CTCCTGGCTGGCTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA220580.1281355688027242RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA219810.1276882735448672RNA PCR Primer, Index 1 (100% over 28bp)
ATCTCGGTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA218510.12693309973290084RNA PCR Primer, Index 1 (100% over 32bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA217350.1262592523314539RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA213940.12427837333237292RNA PCR Primer, Index 1 (100% over 28bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA205170.11918385461626134RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC199790.11605859684058514RNA PCR Primer, Index 2 (100% over 34bp)
TGAATCCAGCGATCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT195560.11360137743703302RNA PCR Primer, Index 1 (100% over 30bp)
TGGCTCAGTTCAGCAGGAACAGTTGGAATTCTCGGGTGCCAAGGAACTCC186140.10812927181493825RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC183610.10665958739626526RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA180620.10492268762874261RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT175870.10216339870040396RNA PCR Primer, Index 1 (100% over 30bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA175830.10214016258311269RNA PCR Primer, Index 1 (100% over 32bp)
TACCACAGGGTAGAACCACGGATGGAATTCTCGGGTGCCAAGGAACTCCA175280.10182066597035769RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC172580.10025222805319677RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph