FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15776354
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA7921835.021331291120877RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA6349014.02438358064227RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT6283453.9828277179885796RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG3812972.416889225482643RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2961251.8770179725936678RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGC2509291.5905385997296968RNA PCR Primer, Index 21 (100% over 50bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1818691.1527948726302668RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1654011.0484108051835044RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1544040.9787052192160495RNA PCR Primer, Index 1 (100% over 32bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1465530.9289408693542247RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1379950.8746951291787698RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1211170.7677122356661115RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1175850.745324299898443RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1144130.7252182601886342RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1119520.7096189651930985RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1098290.6961621170518867RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1068770.6774505693774366RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1018730.645732214173186RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA874860.5545387736608852RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG826610.5239550278854037RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA810160.5135280306210167RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT730060.4627558433336372RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG726030.4602013874688664RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA708970.44938773559467543RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC703700.4460472933099752RNA PCR Primer, Index 1 (100% over 33bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT691150.43809235010826963RNA PCR Primer, Index 1 (97% over 35bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG685730.4346568288211585RNA PCR Primer, Index 6 (100% over 34bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG646500.4097905003906479RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC635080.4025518190071039RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT620510.39331647857293267RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT565050.35816260208157097RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG540310.34248090528394587RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA533410.338107271173048RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC502560.318552689677222RNA PCR Primer, Index 1 (100% over 33bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC481630.3052859995408318RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC469790.2977810969505375RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT466450.2956640044968565RNA PCR Primer, Index 9 (97% over 36bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT463770.2939652596537831No Hit
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA454430.2880450071036692RNA PCR Primer, Index 1 (100% over 32bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT449460.28489472282379064RNA PCR Primer, Index 9 (97% over 36bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA440020.27891108427206945RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC427150.27075330586522084RNA PCR Primer, Index 1 (100% over 27bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG425650.2698025158411126Illumina Small RNA Adapter 2 (100% over 21bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT413740.2622532430496932RNA PCR Primer, Index 1 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA413320.2619870218429429RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC412040.26117568102237054RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC411450.260801703612888RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT404650.25649145550359737RNA PCR Primer, Index 9 (97% over 36bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT388160.2460391038385675RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA375000.23769750602705797RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA368970.23387533013014286RNA PCR Primer, Index 1 (100% over 28bp)
AACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC364650.23113705486071118RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA352340.22333423806286296RNA PCR Primer, Index 1 (100% over 32bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG346200.21944233756417988RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA339630.21527787725858588RNA PCR Primer, Index 1 (100% over 32bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT339370.21511307365440707RNA PCR Primer, Index 9 (97% over 36bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA336400.21323050940667282RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA336270.21314810760458344RNA PCR Primer, Index 1 (100% over 28bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT332450.21072676234318777RNA PCR Primer, Index 1 (100% over 30bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG318260.2017322887151239Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA309710.19631278557770698RNA PCR Primer, Index 1 (100% over 28bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC300990.19078552623755782RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC300810.19067143143466483RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA297110.18832614937519784RNA PCR Primer, Index 1 (100% over 28bp)
TCTCACACAGAAATCGCACCCGTGGAATTCTCGGGTGCCAAGGAACTCCA297080.18830713357471568RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG297020.18826910197375135RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA293170.18582874091187357RNA PCR Primer, Index 1 (100% over 28bp)
GCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT291260.18461806828117575RNA PCR Primer, Index 21 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG287470.18221573882026226RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC279470.17714485869168503RNA PCR Primer, Index 1 (100% over 27bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA273050.17307547738850182RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA266610.16899341888499714RNA PCR Primer, Index 1 (100% over 32bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA261110.16550718879660029No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC257220.1630414733340796RNA PCR Primer, Index 1 (100% over 26bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG248530.1575332297944126RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT246960.1565380695691793RNA PCR Primer, Index 1 (100% over 30bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT240490.15243699526519247RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG238710.15130872443658402RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT233360.147917573350598RNA PCR Primer, Index 21 (100% over 37bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC230530.14612374950511378RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG229470.1454518578880773RNA PCR Primer, Index 21 (100% over 39bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC227300.14407638165320072RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTATGGAATTCTCGGGTGCCAAGGAACTCCA226430.14352492343921794RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC224070.14202901380128768RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC221660.14050141116255377RNA PCR Primer, Index 1 (100% over 31bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC221170.14019081975467843RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG216700.1373574654828359No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT215800.13678699146837095RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC215410.13653978606210282RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG213890.13557631883767313RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG213160.13511360102594047RNA PCR Primer, Index 6 (100% over 34bp)
CAAAGAATTCTCCTTTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG206830.13110126712420372RNA PCR Primer, Index 1 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG205600.13032161930443498Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCCCAACCCTTGTACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC203550.12902220627148706RNA PCR Primer, Index 1 (100% over 31bp)
CTGCTGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT200840.12730444562793153RNA PCR Primer, Index 21 (100% over 37bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA200710.12722204382584215RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT199750.12661353821041288RNA PCR Primer, Index 1 (97% over 35bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC197260.12503522677039322RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTCCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA195100.12366608913567737RNA PCR Primer, Index 1 (100% over 32bp)
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT191540.1214095474784605RNA PCR Primer, Index 1 (97% over 35bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA188150.1192607620239759RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAGTGATGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT188070.11921005322269013RNA PCR Primer, Index 21 (100% over 37bp)
GCATTGGTGGTTCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT186060.11793599459038508RNA PCR Primer, Index 1 (97% over 35bp)
AGGTCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG184930.11721973277222354RNA PCR Primer, Index 21 (100% over 39bp)
ATCTCGGTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA184300.11682040096209809RNA PCR Primer, Index 1 (100% over 32bp)
CGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT182640.11576819333541831RNA PCR Primer, Index 9 (97% over 36bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA182380.11560338973123956RNA PCR Primer, Index 1 (100% over 28bp)
GTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCCA177930.11278271265971847RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC175890.11148963822693128RNA PCR Primer, Index 21 (100% over 44bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA171580.10875770155766029RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC170630.10815553454239174RNA PCR Primer, Index 1 (100% over 33bp)
CCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG169300.10731250072101577RNA PCR Primer, Index 6 (100% over 34bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC169300.10731250072101577RNA PCR Primer, Index 1 (100% over 33bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC167320.10605745788919291RNA PCR Primer, Index 21 (100% over 38bp)
ATCCTGCCGACTACGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA165780.10508131346444179RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA163820.10383894783294037RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG162940.10328115101879687No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC161110.10212118718938483RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAG252.8431223E-669.998135
GTGGTCC150.002225834269.9967963
ACGCAGT150.002226813769.989049
AATGTTA207.937895E-569.989047
CATGGTT1900.069.9890312
CAGATAG207.938269E-569.9883717
TCTCCCA1634100.069.890741
AAGCTGC202150.069.871991
TAGCTTA305900.069.856181
TCCCAAC1645200.069.822363
CCCAACC1642650.069.819744
CTCCCAA1644950.069.807252
AGCTTAT307200.069.797922
GTACCAG1627250.069.7974214
TAGCAGC225150.069.794611
TACCAGT1604800.069.7945215
CCAGTGT916000.069.7935317
CCTTGTA1651950.069.79034410
CTTGTAC1654550.069.7864311
CCCTTGT1652100.069.783579