FastQCFastQC Report
Wed 27 Apr 2022
EGAF00006135198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22087232
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC12864795.824536999475534RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA10131834.587188652702158RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA8877534.019304003326447RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG6537532.959868398176829RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA5321192.4091701486179886RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA5026502.2757491749079284RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5015102.2705878219597637RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3978411.8012261563603804RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC3754441.6998236809392866RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3740651.6935802548730414RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC3407381.542692176185771RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC3381711.5310700770472279RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC3321901.5039910840797073RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA3082651.395670584707038RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3039421.3760981910272867RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2185910.9896713178002567RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG2140230.9689896859869086RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC2038860.9230943922715168RNA PCR Primer, Index 24 (100% over 50bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1718100.7778702193194693RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1550270.7018851434167939RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG1421150.6434260300249484RNA PCR Primer, Index 2 (97% over 35bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1189400.5385011575918612RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1171650.5304648405015169RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1154010.5224783259396197RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC1102930.49935184272977257RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGT1094880.49570720314795447RNA PCR Primer, Index 9 (97% over 36bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC958260.4338524628165268RNA PCR Primer, Index 1 (100% over 26bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA897800.40647918218090884RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC877980.39750567205523984RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC877760.39740606699834546RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA863220.39082307823814233RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA832300.3768240402418918RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA826770.3743203313117732RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA820030.37126879456873546RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC818520.3705851416782329RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT780260.3532629167837781RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC735390.3329480126799048RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC735350.3329299026695604RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT711800.3222676340792726No Hit
AACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA707690.32040683051638164RNA PCR Primer, Index 1 (100% over 32bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA662050.2997433087133779RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC629170.28485688021025RNA PCR Primer, Index 1 (100% over 33bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC609040.2757430175044116RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG594830.26930943632955007RNA PCR Primer, Index 1 (100% over 29bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG585970.26529806903825703RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC557540.2524263791859478RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC539150.24410030193009247RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG506450.22929536847351448RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA465640.2108186304196017RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA455670.20630471034125053RNA PCR Primer, Index 1 (100% over 32bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC428830.1941528934001327RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA410100.18567288105634966RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC405120.18341818476846714RNA PCR Primer, Index 1 (100% over 27bp)
TCGTTTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA397010.17974638017113237RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGC391660.17732416628756378RNA PCR Primer, Index 24 (100% over 39bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTA385430.1745035321764176RNA PCR Primer, Index 22 (97% over 37bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT381000.17249784853077108RNA PCR Primer, Index 1 (100% over 30bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA374860.16971796194289987RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCACTCCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA370690.1678299933644922RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA357410.16181746993013882RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC349350.15816830284573458RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG341190.15447386073546926RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA337770.1529254548510198RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC335890.15207428436483122RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC334270.1513408289458815RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG332110.15036288838728185RNA PCR Primer, Index 1 (100% over 29bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC331630.1501455682631486RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC326730.14792709199595494RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC324840.14707139400718025RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG324320.14683596387270256RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT314680.14247145137969303RNA PCR Primer, Index 1 (100% over 25bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG313980.14215452619866537RNA PCR Primer, Index 6 (100% over 34bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA312710.14157953337022947RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC310010.1403571076719799RNA PCR Primer, Index 1 (100% over 27bp)
TCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC307780.1393474745952775RNA PCR Primer, Index 1 (100% over 33bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG307610.13927050705131364RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG306680.13884944931080545RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG305250.13820201644099178RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA303530.13742328599618098RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG303520.13741875849359483RNA PCR Primer, Index 1 (100% over 29bp)
AATGACACGATCACTCCCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA292370.13237059311008278RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA283200.12821887323862038RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA273390.123777393201647RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA266740.1207666039818842RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC264490.11974791590000956RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA258280.11693633679403558RNA PCR Primer, Index 1 (100% over 28bp)
CTTTCAGTCGGATGTTTACAGCTGGAATTCTCGGGTGCCAAGGAACTCCA251590.11390743756392835RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA251360.11380330500444782RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC250800.11354976485962569RNA PCR Primer, Index 1 (100% over 27bp)
TGTAGTGTTTCCTACTTTATGGATGGAATTCTCGGGTGCCAAGGAACTCC244700.1107879882820989RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG244370.11063858069675729RNA PCR Primer, Index 6 (100% over 34bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGC244360.11063405319417118RNA PCR Primer, Index 24 (100% over 39bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC242370.1097330801795354RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC241830.10948859503988549RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC236690.10716145871062521RNA PCR Primer, Index 1 (100% over 26bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG235080.1064325307942616Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCATCACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACTC232640.10532782016325087RNA PCR Primer, Index 1 (100% over 26bp)
CTCCTGGCTGGCTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA231070.1046170022572317RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG230960.10456719972878449RNA PCR Primer, Index 2 (97% over 35bp)
AGGTCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGC227020.10278336370985734RNA PCR Primer, Index 24 (100% over 39bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC226910.10273356118141014RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT226610.10259773610382687RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC222570.10076862505903864RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph